Alignments for a candidate for aroA in Clostridium kluyveri DSM 555

GapMind for Amino acid biosynthesis

Alignments for a candidate for aroA in Clostridium kluyveri DSM 555

Align 3-phosphoshikimate 1-carboxyvinyltransferase; EC 2.5.1.19; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS (uncharacterized)
to candidate WP_011989355.1 CKL_RS03860 3-phosphoshikimate 1-carboxyvinyltransferase

Query= curated2:B9DZT0
         (426 letters)



>NCBI__GCF_000016505.1:WP_011989355.1
          Length = 426

 Score =  845 bits (2182), Expect = 0.0
 Identities = 426/426 (100%), Positives = 426/426 (100%)

Query: 1   MKYVSINPTKLEGQVKIPPSKSVCHRALICASLSSGVSNITNVDFSEDIEATCEALKSLG 60
           MKYVSINPTKLEGQVKIPPSKSVCHRALICASLSSGVSNITNVDFSEDIEATCEALKSLG
Sbjct: 1   MKYVSINPTKLEGQVKIPPSKSVCHRALICASLSSGVSNITNVDFSEDIEATCEALKSLG 60

Query: 61  VIIEKGNNSLSIKGNSNLYVKKPNVHCFQSGSTLRFLIPLAATLGEEITFTGEGKLVERP 120
           VIIEKGNNSLSIKGNSNLYVKKPNVHCFQSGSTLRFLIPLAATLGEEITFTGEGKLVERP
Sbjct: 61  VIIEKGNNSLSIKGNSNLYVKKPNVHCFQSGSTLRFLIPLAATLGEEITFTGEGKLVERP 120

Query: 121 LNVYYDIFKSQKIYYKTESGKLPLTINGKLKSGDYRVRGDVSSQFVTGLLFALPLLSGDS 180
           LNVYYDIFKSQKIYYKTESGKLPLTINGKLKSGDYRVRGDVSSQFVTGLLFALPLLSGDS
Sbjct: 121 LNVYYDIFKSQKIYYKTESGKLPLTINGKLKSGDYRVRGDVSSQFVTGLLFALPLLSGDS 180

Query: 181 KIEITTELESKSYVDITIDMLGKFGVCVDGSSYREFIIKGNQTYKEVDCNIEGDFSQVAF 240
           KIEITTELESKSYVDITIDMLGKFGVCVDGSSYREFIIKGNQTYKEVDCNIEGDFSQVAF
Sbjct: 181 KIEITTELESKSYVDITIDMLGKFGVCVDGSSYREFIIKGNQTYKEVDCNIEGDFSQVAF 240

Query: 241 WLVMGALGKGITCMGLDTDSLQGDRIIVHILKDMGVEIEEKENCIEVNPSKTTGVVIDVS 300
           WLVMGALGKGITCMGLDTDSLQGDRIIVHILKDMGVEIEEKENCIEVNPSKTTGVVIDVS
Sbjct: 241 WLVMGALGKGITCMGLDTDSLQGDRIIVHILKDMGVEIEEKENCIEVNPSKTTGVVIDVS 300

Query: 301 QCPDLVPVLAALASVSHGTTEIINAARLRIKESDRLKAITSELNKIGAEVIEKEDSLIIH 360
           QCPDLVPVLAALASVSHGTTEIINAARLRIKESDRLKAITSELNKIGAEVIEKEDSLIIH
Sbjct: 301 QCPDLVPVLAALASVSHGTTEIINAARLRIKESDRLKAITSELNKIGAEVIEKEDSLIIH 360

Query: 361 GKENLKGGNVTSWKDHRIAMALAAVSSKCTEPLVIEGAECVKKSYPGFWEDFRSLGGEID 420
           GKENLKGGNVTSWKDHRIAMALAAVSSKCTEPLVIEGAECVKKSYPGFWEDFRSLGGEID
Sbjct: 361 GKENLKGGNVTSWKDHRIAMALAAVSSKCTEPLVIEGAECVKKSYPGFWEDFRSLGGEID 420

Query: 421 EWSVGK 426
           EWSVGK
Sbjct: 421 EWSVGK 426


Lambda     K      H
   0.316    0.135    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 896
Number of extensions: 31
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 426
Length of database: 426
Length adjustment: 32
Effective length of query: 394
Effective length of database: 394
Effective search space:   155236
Effective search space used:   155236
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 51 (24.3 bits)

Align candidate WP_011989355.1 CKL_RS03860 (3-phosphoshikimate 1-carboxyvinyltransferase)
to HMM TIGR01356 (aroA: 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01356.hmm
# target sequence database:        /tmp/gapView.3063004.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01356  [M=415]
Accession:   TIGR01356
Description: aroA: 3-phosphoshikimate 1-carboxyvinyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   4.3e-122  393.8   0.0   4.9e-122  393.6   0.0    1.0  1  NCBI__GCF_000016505.1:WP_011989355.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000016505.1:WP_011989355.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  393.6   0.0  4.9e-122  4.9e-122       1     413 [.      13     416 ..      13     418 .. 0.97

  Alignments for each domain:
  == domain 1  score: 393.6 bits;  conditional E-value: 4.9e-122
                             TIGR01356   1 geikipgsKSishRalllaaLaegetvvtnlLkseDtlatlealrklGakveeekeelviegvgglkepeael 73 
                                           g++kip+sKS+ hRal+ a+L++g + +tn+  seD++at eal++lG+ +e+++++l i+g+ +l  ++  +
  NCBI__GCF_000016505.1:WP_011989355.1  13 GQVKIPPSKSVCHRALICASLSSGVSNITNVDFSEDIEATCEALKSLGVIIEKGNNSLSIKGNSNLYVKKPNV 85 
                                           79**************************************************99**********99999999* PP

                             TIGR01356  74 dlgnsGttaRlltgvlalasgevvltgdeslkkRPierlveaLrelgaeieskeeegslPlaisgplkggive 146
                                           ++ +sG t+R+l++++a++++e+++tg+ +l +RP++   +  ++ +  i +k+e+g+lPl+i+g+lk+g  +
  NCBI__GCF_000016505.1:WP_011989355.1  86 HCFQSGSTLRFLIPLAATLGEEITFTGEGKLVERPLNVYYDIFKSQK--IYYKTESGKLPLTINGKLKSGDYR 156
                                           ********************************************975..56666779**************** PP

                             TIGR01356 147 lsgsaSsQyksalllaaplalqavtleivgeklisrpyieitLkllksfgveveeederkivvkggqkykqke 219
                                           ++g++SsQ++++ll+a pl + ++++ei++ +l s++y++it+++l++fgv v+ +  r++++kg+q+yk+ +
  NCBI__GCF_000016505.1:WP_011989355.1 157 VRGDVSSQFVTGLLFALPLLSGDSKIEITT-ELESKSYVDITIDMLGKFGVCVDGSSYREFIIKGNQTYKEVD 228
                                           ******************999999999999.****************************************** PP

                             TIGR01356 220 vevegDaSsAafflaaaaitgeevtvenlgenstqgdkaiiivLeemGadveveeqrdvevegasklkgvkvd 292
                                           +++egD+S  af+l+++a+ +  +t  +l ++s+qgd+ i+ +L+ mG ++e +e+  +ev+ +sk+ gv   
  NCBI__GCF_000016505.1:WP_011989355.1 229 CNIEGDFSQVAFWLVMGALGK-GITCMGLDTDSLQGDRIIVHILKDMGVEIEEKEN-CIEVN-PSKTTGVV-- 296
                                           *******************99.9**************77777**************.8****.78899***.. PP

                             TIGR01356 293 idvdsliDelptlavlaafAegetriknieelRvkEsdRiaaiaeeLeklGveveeledgllieGkkkelkga 365
                                           idv++++D++p+la+la++  g+t+i n+++lR+kEsdR++ai++eL+k+G+ev e+ed+l+i+Gk ++lkg+
  NCBI__GCF_000016505.1:WP_011989355.1 297 IDVSQCPDLVPVLAALASVSHGTTEIINAARLRIKESDRLKAITSELNKIGAEVIEKEDSLIIHGK-ENLKGG 368
                                           ******************************************************************.6***** PP

                             TIGR01356 366 vvdtydDHRiamalavlglaaegeveiedaecvaksfPeFfevleqlg 413
                                           +v +++DHRiamala ++ ++ +++ ie+aecv+ks+P F+e+ ++lg
  NCBI__GCF_000016505.1:WP_011989355.1 369 NVTSWKDHRIAMALAAVSSKCTEPLVIEGAECVKKSYPGFWEDFRSLG 416
                                           ********************************************9997 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (415 nodes)
Target sequences:                          1  (426 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 28.54
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis