Alignments for a candidate for prpE in Crocosphaera subtropica ATCC 51142

GapMind for Amino acid biosynthesis

Alignments for a candidate for prpE in Crocosphaera subtropica ATCC 51142

Align Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 (characterized)
to candidate WP_009545819.1 CCE_RS09565 acetate--CoA ligase

Query= SwissProt::P31638
         (660 letters)



>NCBI__GCF_000017845.1:WP_009545819.1
          Length = 659

 Score =  725 bits (1871), Expect = 0.0
 Identities = 365/658 (55%), Positives = 471/658 (71%), Gaps = 13/658 (1%)

Query: 4   IESVMQEHRVFNPPEGFASQAAIPSMEAYQALCDEAERDYEGFWARHARELLHWTKPFTK 63
           IESV+QE RVFNPP  FA+ A I S++ Y+ L ++A+ D E FWA  A + L+W + + +
Sbjct: 10  IESVLQEKRVFNPPADFATNAHIKSLQQYEELYEKAKNDPETFWAELAEQELYWFEKWDQ 69

Query: 64  VLDQSNAPFYKWFEDGELNASYNCLDRNLQNGNADKVAIVFEADDGSVTRVTYRELHGKV 123
           VLD  N P  KWF +G+LN SYNCLDR+L     +K AI++E + G    +TY ELH +V
Sbjct: 70  VLDW-NPPTAKWFVNGKLNMSYNCLDRHLTTWRRNKAAIIWEGEPGDSRTLTYGELHREV 128

Query: 124 CRFANGLKALGIRKGDRVVIYMPMSVEGVVAMQACARLGATHSVVFGGFSAKSLQERLVD 183
           C+FAN LK +G++KGDRV IYMPM  E  +AM AC R+GA HSVVFGGFSA++L++RL D
Sbjct: 129 CQFANVLKDMGVQKGDRVGIYMPMVPEAAIAMLACTRIGAPHSVVFGGFSAEALRDRLED 188

Query: 184 VGAVALITADEQMRGGKALPLKAIADDALALGGCEAVRNVIVYRRTGGKVAWTEGRDRWM 243
             A  +ITAD   R  K +PLK   D AL   G  +V  VIV +RT   V   EGRD W 
Sbjct: 189 AEAKVVITADGGFRKDKIVPLKIEVDQALE-NGASSVEKVIVVQRTKQDVTMREGRDYWW 247

Query: 244 EDVSAGQPDTCEAEPVSAEHPLFVLYTSGSTGKPKGVQHSTGGYLLWALMTMKWTFDIKP 303
            D+       C+AE + +E PLF+LYTSGSTGKPKGV H+TGGY L+  MT KW FD+K 
Sbjct: 248 HDLQKDASPKCDAEVMDSEDPLFILYTSGSTGKPKGVVHTTGGYNLYTHMTTKWIFDLKD 307

Query: 304 DDLFWCTADIGWVTGHTYIAYGPLAAGATQVVFEGVPTYPNAGRFWDMIARHKVSIFYTA 363
            D++WCTAD+GW+TGH+YI YGPL+ GAT +++EGVP   N G FWD++ ++ V++FYTA
Sbjct: 308 TDVYWCTADVGWITGHSYIVYGPLSNGATSLMYEGVPRPSNPGCFWDVVEKYGVNVFYTA 367

Query: 364 PTAIRSLIKAAEADEKIHPKQYDLSSLRLLGTVGEPINPEAWMWYYKNIGNERCPIVDTF 423
           PTAIR+ +K  E      P   DLSSLRLLGTVGEPINPEAWMWY++ IG E+CPIVDT+
Sbjct: 368 PTAIRAFMKMGEE----LPNARDLSSLRLLGTVGEPINPEAWMWYHRVIGGEKCPIVDTW 423

Query: 424 WQTETGGHMITPLPGATPLVPGSCTLPLPGIMAAIVDETGHDVPNGNGGILVVKRPWPAM 483
           WQTETGG MITPLPGATP  PGS T P PGI+A +VD+ G+ VPN  GG LV+K PWP+M
Sbjct: 424 WQTETGGIMITPLPGATPTKPGSATRPFPGIIADVVDKDGNPVPNNAGGYLVIKHPWPSM 483

Query: 484 IRTIWGDPERFRKSYF---PEELGGKLYLAGDGSIRDKDTGYFTIMGRIDDVLNVSGHRM 540
           +RT++ +PERF+K+Y+   PE  G  +Y AGD S R  + GYF IMGR DDV+NVSGHR+
Sbjct: 484 LRTVYNNPERFQKTYWDPIPEVEGKPVYFAGD-SARKDENGYFWIMGRTDDVMNVSGHRL 542

Query: 541 GTMEIESALVSNPLVAEAAVVGRPDDMTGEAICAFVVLKRSRPTGEEAVKIATELRNWVG 600
           G+ME+ESALVS+P VAEA+VVG P ++ GE I AFV LK +    +E  K   EL+  V 
Sbjct: 543 GSMELESALVSHPAVAEASVVGVPHEIKGEDIYAFVTLKDNYSPSDELNK---ELKQHVV 599

Query: 601 KEIGPIAKPKDIRFGDNLPKTRSGKIMRRLLRSLAKGEEITQDTSTLENPAILEQLKQ 658
            +I  IA+P  I+F + LPKTRSGKI+RR LR +A G++I  D ST+ +P++L+QL++
Sbjct: 600 TKISAIARPGTIQFAEALPKTRSGKIVRRFLRQIAAGQDIVGDKSTVVDPSVLDQLRE 657


Lambda     K      H
   0.319    0.136    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1388
Number of extensions: 74
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 660
Length of database: 659
Length adjustment: 38
Effective length of query: 622
Effective length of database: 621
Effective search space:   386262
Effective search space used:   386262
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis