Alignments for a candidate for asd in Nostoc punctiforme PCC 73102 ATCC 29133; PCC 73102

GapMind for Amino acid biosynthesis

Alignments for a candidate for asd in Nostoc punctiforme PCC 73102 ATCC 29133; PCC 73102

Align aspartate semialdehyde dehydrogenase (EC 1.2.1.11) (characterized)
to candidate WP_012407608.1 NPUN_RS04305 aspartate-semialdehyde dehydrogenase

Query= metacyc::AT1G14810-MONOMER
         (375 letters)



>NCBI__GCF_000020025.1:WP_012407608.1
          Length = 345

 Score =  348 bits (894), Expect = e-100
 Identities = 173/335 (51%), Positives = 240/335 (71%), Gaps = 8/335 (2%)

Query: 41  LAVVGVTGAVGQEFLSVLSDRDFPYSSIKMLASKRSAGKRVAFDGHEYTVEELTADSFNG 100
           LA++G TGAVG E L +L  R+FP + +K+LAS+RS G+ + F G    VE ++  +F  
Sbjct: 7   LAILGATGAVGTELLELLESRNFPIADLKLLASERSIGRSLRFKGENIPVEPVSDRAFEN 66

Query: 101 VDIALFSAGGSISKEFGPLAAEKGTIVVDNSSAFRMVDGVPLVIPEVNPEAMKGIKVGMG 160
           VDI L SAGGS SK +  +A  KG +V+DNSSAFRM   VPL++PEVNP+A    +    
Sbjct: 67  VDIVLASAGGSTSKTWAAVAVAKGAVVIDNSSAFRMNPEVPLIVPEVNPQAAANHQ---- 122

Query: 161 KGALIANPNCSTIICLMAVTPLHHHAKVKRMVVSTYQAASGAGAAAMEELVQQTREVLEG 220
              +IANPNC+TI+  +AV PLH    V+R+V STYQ+ASGAGA A+ E+  QT  +L+G
Sbjct: 123 --GIIANPNCTTILMTVAVWPLHKVKPVQRIVASTYQSASGAGARAIAEVKTQTSAILQG 180

Query: 221 KPPTCNIFGQQYAFNLFSHNAPILDNGYNEEEMKLVKETRKIWNDTEVKVTATCIRVPVM 280
           +PP   +     AFNLF HN+P+ D GY EEEMK+V ETRKI+   ++++TATCIRVPV+
Sbjct: 181 QPPVAEVLPYPLAFNLFPHNSPLNDLGYCEEEMKMVNETRKIFGTQQIRITATCIRVPVL 240

Query: 281 RAHAESVNLQFENPLDENTAREILKKAPGVYIIDDRASNTFPTPLDVSNKDDVAVGRIRR 340
           RAH+E++NL+FE       AREIL  +PGV +++D  +N FP P++ + +D+V VGRIR+
Sbjct: 241 RAHSEAINLEFETTFSAEEAREILNSSPGVKLVEDWETNHFPMPIEATGRDEVLVGRIRQ 300

Query: 341 DVSQDGNFGLDIFVCGDQIRKGAALNAVQIAEMLL 375
           D+S   + GL++++CGDQIRKGAALNA+QIAE+L+
Sbjct: 301 DISH--SCGLELWLCGDQIRKGAALNAIQIAELLV 333


Lambda     K      H
   0.318    0.133    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 322
Number of extensions: 11
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 375
Length of database: 345
Length adjustment: 29
Effective length of query: 346
Effective length of database: 316
Effective search space:   109336
Effective search space used:   109336
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 49 (23.5 bits)

Align candidate WP_012407608.1 NPUN_RS04305 (aspartate-semialdehyde dehydrogenase)
to HMM TIGR01296 (asd: aspartate-semialdehyde dehydrogenase (EC 1.2.1.11))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01296.hmm
# target sequence database:        /tmp/gapView.2546620.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01296  [M=339]
Accession:   TIGR01296
Description: asd_B: aspartate-semialdehyde dehydrogenase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   1.9e-128  414.3   0.0   2.2e-128  414.0   0.0    1.0  1  NCBI__GCF_000020025.1:WP_012407608.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000020025.1:WP_012407608.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  414.0   0.0  2.2e-128  2.2e-128       2     338 ..       7     334 ..       6     335 .. 0.98

  Alignments for each domain:
  == domain 1  score: 414.0 bits;  conditional E-value: 2.2e-128
                             TIGR01296   2 vaivGatGavGqellkvLeernfpidklvllasersaGkkvkfkgkeleveeaekesfegidialfsaGgsvs 74 
                                           +ai+GatGavG ell++Le rnfpi +l+llasers G+ ++fkg+++ ve ++   fe++di l saGgs+s
  NCBI__GCF_000020025.1:WP_012407608.1   7 LAILGATGAVGTELLELLESRNFPIADLKLLASERSIGRSLRFKGENIPVEPVSDRAFENVDIVLASAGGSTS 79 
                                           79*********************************************************************** PP

                             TIGR01296  75 kefapkaakagviviDntsafrldedvPLvvpevnaeelkeakkkgiianPnCstiqlvvvLkplkdeaklkr 147
                                           k++a+ a+++g++viDn+safr++++vPL+vpevn +  ++++  giianPnC+ti ++v++ pl++   ++r
  NCBI__GCF_000020025.1:WP_012407608.1  80 KTWAAVAVAKGAVVIDNSSAFRMNPEVPLIVPEVNPQAAANHQ--GIIANPNCTTILMTVAVWPLHKVKPVQR 150
                                           **************************************99999..**************************** PP

                             TIGR01296 148 vvvstYqavsGaGkkgveeLknqtkavlegkekepeidalkakkfakqiafnaiplidklkedGytkeelkll 220
                                           +v stYq+ sGaG+++++e+k qt a+l+g+        + a+  ++++afn++p+   l++ Gy +ee+k++
  NCBI__GCF_000020025.1:WP_012407608.1 151 IVASTYQSASGAGARAIAEVKTQTSAILQGQP-------PVAEVLPYPLAFNLFPHNSPLNDLGYCEEEMKMV 216
                                           ***************************99863.......35899***************************** PP

                             TIGR01296 221 fetrkilgiedlkvsatcvrvPvftghsesvsiefekelsveevkelLkeapgvvviddpsenlyptPleavg 293
                                           +etrki+g+++++++atc+rvPv+++hse++++efe  +s+ee++e+L+ +pgv++++d + n +p+P+ea+g
  NCBI__GCF_000020025.1:WP_012407608.1 217 NETRKIFGTQQIRITATCIRVPVLRAHSEAINLEFETTFSAEEAREILNSSPGVKLVEDWETNHFPMPIEATG 289
                                           ************************************************************************* PP

                             TIGR01296 294 kdevfvgrirkDlskekglalfvvaDnlrkGaalnavqiaellik 338
                                           +dev+vgrir+D+s++ gl+l++ +D++rkGaalna+qiaell++
  NCBI__GCF_000020025.1:WP_012407608.1 290 RDEVLVGRIRQDISHSCGLELWLCGDQIRKGAALNAIQIAELLVE 334
                                           ******************************************986 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (339 nodes)
Target sequences:                          1  (345 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 12.51
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis