Alignments for a candidate for carA in Trichlorobacter lovleyi SZ

GapMind for Amino acid biosynthesis

Alignments for a candidate for carA in Trichlorobacter lovleyi SZ

Align Carbamoyl-phosphate synthase small chain; Carbamoyl-phosphate synthetase glutamine chain; EC 6.3.5.5 (characterized)
to candidate WP_012470138.1 GLOV_RS10325 glutamine-hydrolyzing carbamoyl-phosphate synthase small subunit

Query= SwissProt::P0A6F1
         (382 letters)



>NCBI__GCF_000020385.1:WP_012470138.1
          Length = 373

 Score =  384 bits (986), Expect = e-111
 Identities = 203/374 (54%), Positives = 251/374 (67%), Gaps = 3/374 (0%)

Query: 5   ALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNVG 64
           A+L L DG  F G++ GA G A GEVVFNT+MTGYQE+LTDPSY  Q+VT+TY  IGN G
Sbjct: 3   AILALADGRIFEGKSFGAGGEASGEVVFNTAMTGYQEVLTDPSYKGQMVTMTYTQIGNTG 62

Query: 65  TNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYLKRHNIVAIADIDTRKLTRLLRE 124
            N  D ES  +   G ++R+     SNFR+T  L +YLK + +V I  +DTR LTR LR+
Sbjct: 63  INPEDIESRGLFLSGFIVREYLDFPSNFRSTMSLDAYLKENGVVGIHGLDTRALTRHLRD 122

Query: 125 KGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAKEVTTAEAYSWTQGSWTLTGGLPEA 184
           KGAQNG I   D   A+L + KARA P + G+DLA  V+  + Y WT+G+W L  G P+ 
Sbjct: 123 KGAQNGIISTVDFDHASL-VAKARAIPSMAGLDLASGVSCDKPYHWTEGAWKLGEGYPQI 181

Query: 185 KKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTIVPAQTSAEDVLKMNPDGIFLSNGPGD 244
                L + VVAYDFG K NILR LVD GC +T+VPA   AE+ L MNPDGIFLSNGPGD
Sbjct: 182 DPA-TLKYKVVAYDFGIKYNILRCLVDAGCDVTVVPATFPAEEALAMNPDGIFLSNGPGD 240

Query: 245 PAPCDYAITAIQKFLETDIPVFGICLGHQLLALASGAKTVKMKFGHHGGNHPVKDVEKNV 304
           P P       I+KF+    PVFGICLGHQLL LA G +TVK+ FG+HG N PV D+   V
Sbjct: 241 PEPLVAVQQEIRKFIGKK-PVFGICLGHQLLGLALGGRTVKLPFGNHGSNLPVMDMSTRV 299

Query: 305 VMITAQNHGFAVDEATLPANLRVTHKSLFDGTLQGIHRTDKPAFSFQGHPEASPGPHDAA 364
           V IT+QNHGFAVD A+L     + H++L D T++GI     P FS Q HPEASPGPHD+ 
Sbjct: 300 VEITSQNHGFAVDLASLGDVACLGHENLNDQTVEGIRHCTLPVFSVQHHPEASPGPHDSQ 359

Query: 365 PLFDHFIELIEQYR 378
            LF  F+E++EQ+R
Sbjct: 360 YLFGRFVEMMEQHR 373


Lambda     K      H
   0.318    0.135    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 454
Number of extensions: 15
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 382
Length of database: 373
Length adjustment: 30
Effective length of query: 352
Effective length of database: 343
Effective search space:   120736
Effective search space used:   120736
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

Align candidate WP_012470138.1 GLOV_RS10325 (glutamine-hydrolyzing carbamoyl-phosphate synthase small subunit)
to HMM TIGR01368 (carA: carbamoyl-phosphate synthase, small subunit (EC 6.3.5.5))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01368.hmm
# target sequence database:        /tmp/gapView.733686.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01368  [M=361]
Accession:   TIGR01368
Description: CPSaseIIsmall: carbamoyl-phosphate synthase, small subunit
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   6.9e-150  484.9   0.0   7.9e-150  484.7   0.0    1.0  1  NCBI__GCF_000020385.1:WP_012470138.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000020385.1:WP_012470138.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  484.7   0.0  7.9e-150  7.9e-150       1     360 [.       3     370 ..       3     371 .. 0.97

  Alignments for each domain:
  == domain 1  score: 484.7 bits;  conditional E-value: 7.9e-150
                             TIGR01368   1 atlvledGtvfegksfgaekevvGevvFnTsmtGYqEiltDpsYkgqivvltyplignygvneedaeskkikv 73 
                                           a+l+l+dG++fegksfga +e+ GevvFnT+mtGYqE+ltDpsYkgq+v++ty++ign+g+n+ed+es+   +
  NCBI__GCF_000020385.1:WP_012470138.1   3 AILALADGRIFEGKSFGAGGEASGEVVFNTAMTGYQEVLTDPSYKGQMVTMTYTQIGNTGINPEDIESRGLFL 75 
                                           689********************************************************************** PP

                             TIGR01368  74 kglvvkelskevsnyrakesLeeflkeegivaiegvDTRalvkklRekgsmkavisteksekeelvekakesp 146
                                           +g++v+e+ + +sn+r++ sL+++lke+g+v+i+g+DTRal+++lR+kg+++++ist ++++ +lv+ka++ p
  NCBI__GCF_000020385.1:WP_012470138.1  76 SGFIVREYLDFPSNFRSTMSLDAYLKENGVVGIHGLDTRALTRHLRDKGAQNGIISTVDFDHASLVAKARAIP 148
                                           ************************************************************************* PP

                             TIGR01368 147 kvkevnlvkevstkeayeleq..........kakkegkklrvvvidlGvKenilreLvkrgvevtvvpadtsa 209
                                           ++++++l++ vs++++y++++          ++   + k++vv++d+G+K nilr+Lv+ g++vtvvpa+++a
  NCBI__GCF_000020385.1:WP_012470138.1 149 SMAGLDLASGVSCDKPYHWTEgawklgegypQIDPATLKYKVVAYDFGIKYNILRCLVDAGCDVTVVPATFPA 221
                                           ********************98887766554466677779********************************* PP

                             TIGR01368 210 eeikklnpdgillsnGPGdPaaveeaietvkklleakiPifGIclGhqllalalgaktyklkfGhrGaNhpvk 282
                                           ee  ++npdgi+lsnGPGdP+ + ++ ++++k+++ k+P+fGIclGhqll+lalg++t+kl fG++G+N pv+
  NCBI__GCF_000020385.1:WP_012470138.1 222 EEALAMNPDGIFLSNGPGDPEPLVAVQQEIRKFIG-KKPVFGICLGHQLLGLALGGRTVKLPFGNHGSNLPVM 293
                                           ***********************************.************************************* PP

                             TIGR01368 283 dlktgrveitsqNHgyavdeeslkeeelevthvnlnDgtveglehkelpvfsvQyHPeaspGphdteylFdef 355
                                           d++t  veitsqNHg+avd +sl  + +   h nlnD+tveg++h +lpvfsvQ+HPeaspGphd++ylF +f
  NCBI__GCF_000020385.1:WP_012470138.1 294 DMSTRVVEITSQNHGFAVDLASLG-DVACLGHENLNDQTVEGIRHCTLPVFSVQHHPEASPGPHDSQYLFGRF 365
                                           **********************65.678999****************************************** PP

                             TIGR01368 356 velik 360
                                           ve+++
  NCBI__GCF_000020385.1:WP_012470138.1 366 VEMME 370
                                           ***97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (361 nodes)
Target sequences:                          1  (373 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 12.65
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis