Alignments for a candidate for argI in Chlorobium limicola DSM 245

GapMind for Amino acid biosynthesis

Alignments for a candidate for argI in Chlorobium limicola DSM 245

Align ornithine carbamoyltransferase (EC 2.1.3.3) (characterized)
to candidate WP_012466192.1 CLIM_RS06245 ornithine carbamoyltransferase

Query= metacyc::AT1G75330-MONOMER
         (375 letters)



>NCBI__GCF_000020465.1:WP_012466192.1
          Length = 338

 Score =  302 bits (773), Expect = 1e-86
 Identities = 146/306 (47%), Positives = 208/306 (67%), Gaps = 2/306 (0%)

Query: 70  SDLKDFLAIDDFDTATIKTILDKASEVKALLKSGERN--YLPFKGKSMSMIFAKPSMRTR 127
           S  +DFL     D A I  + + +  +K    + + +  +LP K ++++MIF+KPS+RTR
Sbjct: 10  SGKRDFLGFSRLDAAKIIELFEYSLHIKQKRNAPQADVPFLPLKDRTVAMIFSKPSLRTR 69

Query: 128 VSFETGFFLLGGHALYLGPNDIQMGKREETRDVARVLSRYNDIIMARVFAHQDILDLANY 187
           VSFE G   LGG+A+ L    I +G RE   D+AR+LSRYND I+AR+  H  I  LA  
Sbjct: 70  VSFELGIHELGGYAISLEGKSIGVGSRESVEDIARLLSRYNDAIVARLHEHSIIEGLAAN 129

Query: 188 SSVPVVNGLTDHNHPCQIMADALTMIEHIGQVEGTKVVYVGDGNNMVHSWLELASVIPFH 247
           +++PVVN LTD +HPCQ++ADA T+ E     +G KVV+VGDGNN+ +SW+ELA ++PFH
Sbjct: 130 ATIPVVNALTDLSHPCQVLADAFTLYERGLWHDGIKVVFVGDGNNVANSWIELAGILPFH 189

Query: 248 FVCACPKGYEPDKERVSKAKQAGLSKIEITNDPKEAVIGADVVYSDVWASMGQKDEAEAR 307
           FV ACP+GY PD   +  A+ +G+SKIE+ +DP EA  GAD +Y+DVW SMGQ+DE E R
Sbjct: 190 FVLACPEGYTPDSGLLEAARSSGVSKIEVLHDPAEAAAGADALYTDVWTSMGQEDEFEER 249

Query: 308 RKAFQGFQVDEALMKLAGQKAYFMHCLPAERGVEVTNGVVEAPYSIVFPQAENRMHAQNA 367
            + F  FQ++  L++LA   A  MHC+PA RG E+T  V++ P S++  +AENR+H Q A
Sbjct: 250 LRIFSPFQINSDLLRLAKPSAVVMHCMPAHRGQEITAEVMDGPQSVILDEAENRLHVQKA 309

Query: 368 IMLHLL 373
           +++ LL
Sbjct: 310 LLVKLL 315


Lambda     K      H
   0.319    0.132    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 294
Number of extensions: 11
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 375
Length of database: 338
Length adjustment: 29
Effective length of query: 346
Effective length of database: 309
Effective search space:   106914
Effective search space used:   106914
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

Align candidate WP_012466192.1 CLIM_RS06245 (ornithine carbamoyltransferase)
to HMM TIGR00658 (argF: ornithine carbamoyltransferase (EC 2.1.3.3))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00658.hmm
# target sequence database:        /tmp/gapView.2788323.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00658  [M=304]
Accession:   TIGR00658
Description: orni_carb_tr: ornithine carbamoyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
     9e-109  349.3   0.0   1.1e-108  349.0   0.0    1.0  1  NCBI__GCF_000020465.1:WP_012466192.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000020465.1:WP_012466192.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  349.0   0.0  1.1e-108  1.1e-108       1     303 [.      13     315 ..      13     316 .. 0.97

  Alignments for each domain:
  == domain 1  score: 349.0 bits;  conditional E-value: 1.1e-108
                             TIGR00658   1 rhllslldlseeelkellelakklkkekkkgke...ekklkgktlaliFekrstRtRvsfevaayelGaqvly 70 
                                           r++l +  l+ +++ el e++ ++k+++++ +       lk +t+a+iF k+s RtRvsfe ++ elG+ ++ 
  NCBI__GCF_000020465.1:WP_012466192.1  13 RDFLGFSRLDAAKIIELFEYSLHIKQKRNAPQAdvpFLPLKDRTVAMIFSKPSLRTRVSFELGIHELGGYAIS 85 
                                           78999999*******************9998773335689********************************* PP

                             TIGR00658  71 lnkeelqlgrkesikDtarvlsryvdaivvRvykhedveelakyasvPvingLtdlehPcqilaDlltikekl 143
                                           l+ +++ +g +es++D ar lsry daiv+R ++h+++e la +a++Pv+n+Ltdl+hPcq+laD +t++e+ 
  NCBI__GCF_000020465.1:WP_012466192.1  86 LEGKSIGVGSRESVEDIARLLSRYNDAIVARLHEHSIIEGLAANATIPVVNALTDLSHPCQVLADAFTLYERG 158
                                           ************************************************************************9 PP

                             TIGR00658 144 gklkevklvyvGDannvanslllaaaklGldvvvatPeglepeaeivkkakkiakeng.gkleltedpkkavk 215
                                              +++k+v+vGD+nnvans +  a  l +++v+a+Peg++p++ +++ a+     +g +k+e+++dp++a++
  NCBI__GCF_000020465.1:WP_012466192.1 159 LWHDGIKVVFVGDGNNVANSWIELAGILPFHFVLACPEGYTPDSGLLEAARS----SGvSKIEVLHDPAEAAA 227
                                           9**********************************************99965....5549************* PP

                             TIGR00658 216 dadviytDvwvsmGeeekkeerlkllkpyqvneellelakpevkflhCLPavrGeevtdevlegeasivfdea 288
                                           +ad +ytDvw+smG+e++ eerl+++ p+q+n++ll+lakp+++++hC+Pa+rG+e+t ev++g++s++ dea
  NCBI__GCF_000020465.1:WP_012466192.1 228 GADALYTDVWTSMGQEDEFEERLRIFSPFQINSDLLRLAKPSAVVMHCMPAHRGQEITAEVMDGPQSVILDEA 300
                                           ************************************************************************* PP

                             TIGR00658 289 enRlhaqkavlkall 303
                                           enRlh+qka+l+ ll
  NCBI__GCF_000020465.1:WP_012466192.1 301 ENRLHVQKALLVKLL 315
                                           ***********9988 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (304 nodes)
Target sequences:                          1  (338 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 22.24
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the original publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis