Alignments for a candidate for glnA in Chlorobium limicola DSM 245

GapMind for Amino acid biosynthesis

Alignments for a candidate for glnA in Chlorobium limicola DSM 245

Align glutamine synthetase type III subunit (EC 6.3.1.2) (characterized)
to candidate WP_150081556.1 CLIM_RS04625 glutamine synthetase III

Query= metacyc::SYNPCC7942_0169-MONOMER
         (723 letters)



>NCBI__GCF_000020465.1:WP_150081556.1
          Length = 714

 Score =  686 bits (1771), Expect = 0.0
 Identities = 356/715 (49%), Positives = 487/715 (68%), Gaps = 18/715 (2%)

Query: 20  LPSKPPMSLEAIWAENVFDLSKMQARLPKAVFKSIKNTIVTGQKLDPSVADAVATAMKDW 79
           + SK P+S  + +    FD   M+ARLPK  FK++++TI  G+K+   +A  VA  MK+W
Sbjct: 3   MESKVPVS--SYFGAMTFDQKAMRARLPKDEFKALQDTIRAGKKITGEIAGVVAHGMKEW 60

Query: 80  AMSKGALYYAHVFYPMTNVTAEKHDGFISVQGDGKVISEFSGKVLVQGEPDGSSFPNGGI 139
           AM +GA +Y H F PMT  TAEKHD F+S+  DG  I  FSG+ L+QGEPD SSFP+GG+
Sbjct: 61  AMEQGATHYTHWFQPMTGSTAEKHDAFLSIDRDGTPIERFSGEQLIQGEPDASSFPSGGM 120

Query: 140 RDTFEARGYTGWDVTSPAYIMETDNGSTLCIPTVFVSWTGEALDKKVPLLRSIAAMDKAA 199
           R TFEARGYT WD +SPA++M+   G TLCIPTVF+S+ GEALD K PLLRS+ A+  +A
Sbjct: 121 RTTFEARGYTAWDPSSPAFLMKGGTGLTLCIPTVFISYHGEALDSKTPLLRSMDAVSNSA 180

Query: 200 RKVLTLLGNTEVSQVNSSCGAEQEYFLVDANFAAQRPDLLLAGRTLFGRPSAKGQEFDDH 259
            ++L +LG T +S+V +  G EQEYFL+D  F ++RPDL++ GRTL G    KGQ+ +DH
Sbjct: 181 IRLLNVLGTTGISRVKTFAGCEQEYFLIDKKFYSERPDLIMCGRTLLGALPPKGQQLEDH 240

Query: 260 YFGAIPERVQVFMQDVEETLYKLGIPAKTRHNEVAPGQFEIAPFFEAANVASDHQQLIMT 319
           YFG+IP+RV  FMQDVE  LY LGIPAKTRHNEVAP QFEIAP FE AN+A DH  L+M 
Sbjct: 241 YFGSIPDRVLEFMQDVEHELYLLGIPAKTRHNEVAPHQFEIAPIFEEANIAVDHNLLVME 300

Query: 320 VLKQTAKKHGFMCLLHEKPFAGINGSGKHVNWSVGNATQGNLLDPGDSPHDNAQFLVFCG 379
           V+++ A K GF  LL EKPFAGINGSGKH NWS+G  T  NLLDPGD+P +N  FLVF  
Sbjct: 301 VMRKVADKKGFALLLTEKPFAGINGSGKHNNWSIGTDTGINLLDPGDTPTENINFLVFLV 360

Query: 380 AVIRGVHKYGPLMRAAIATASNDHRLGANEAPPAIMSVYLGTQLEEVFEQIKTGTVTESK 439
           AV++GV+K   ++R +IA+  NDHRLGANEAPPA+++V+LG QLE V + I++G V    
Sbjct: 361 AVLKGVYKRADVLRMSIASTGNDHRLGANEAPPAVVTVFLGEQLETVLDAIESGKVDLKT 420

Query: 440 QKGIMDLGVDVLPYLTKDAGDRNRTSPFAFTGNRFEFRAVGASQSVSGPLIVLNTILADS 499
           +K +++LG+  +P L KD  DRNRTSPFAFTGN+FEFRAVG+SQ+ S P +VLNT++A++
Sbjct: 421 EKQVLNLGLSQVPLLNKDYTDRNRTSPFAFTGNKFEFRAVGSSQAASVPNMVLNTLMAEA 480

Query: 500 LDWIGNRLESELAKGLDLNTAILTVLKEVMEEHGNVIFGGNGYSEEWHREAVEKRGLANL 559
           LD + + +E+++A G D ++A+L  ++E +    ++ + G+ YSE   ++A ++RGL NL
Sbjct: 481 LDELTDAIEAKIAAGKDRDSAVLEAIREGITATKDIRYPGDNYSEAL-QQAAKERGLPNL 539

Query: 560 PTTADALPVLKEEYIEDLFKKTGVLTPVELESRFEVYAEQYILSIEVEAKLAVSIAKTII 619
             T  AL  L++  ++ +F K GVLT  E+ESR  +  E+Y+  I++EA+    + KT++
Sbjct: 540 KNTPQALRTLEKSDVKAMFVKYGVLTEQEIESRLNIRLERYVKGIDIEARTLQLMLKTLV 599

Query: 620 YPAAVEYLAKLSGT----IASLSGLG-----IDFEKESARKIAELTSSLVGAATQLSEAL 670
            P   EY   +  +    +A+   +G     I  +    + +AE  SSL+    +L EA+
Sbjct: 600 IPDVSEYQGDIGNSFNNLLAASEAIGLSEGAIASQANHFKNLAENLSSLIDLTAELDEAV 659

Query: 671 EHESSDTVE----HLQYCAKTLRPLMDNVRTYADALEAEVADSFWPLPTYQEMLF 721
             E  +T+E       +CA  L PLM+ VR  AD LE  V  S W LPTY EMLF
Sbjct: 660 --EKIETIEGEFGKADFCADELLPLMNAVRAVADRLELMVDRSRWQLPTYSEMLF 712


Lambda     K      H
   0.316    0.133    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1233
Number of extensions: 51
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 723
Length of database: 714
Length adjustment: 40
Effective length of query: 683
Effective length of database: 674
Effective search space:   460342
Effective search space used:   460342
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis