Alignments for a candidate for cysK in Prosthecochloris aestuarii DSM 271

GapMind for Amino acid biosynthesis

Alignments for a candidate for cysK in Prosthecochloris aestuarii DSM 271

Align cysteine synthase (EC 2.5.1.47) (characterized)
to candidate WP_012506546.1 PAES_RS09995 cysteine synthase A

Query= BRENDA::P9WP55
         (310 letters)



>NCBI__GCF_000020625.1:WP_012506546.1
          Length = 308

 Score =  394 bits (1012), Expect = e-114
 Identities = 200/307 (65%), Positives = 239/307 (77%)

Query: 1   MSIAEDITQLIGRTPLVRLRRVTDGAVADIVAKLEFFNPANSVKDRIGVAMLQAAEQAGL 60
           M++ E IT+ +GRTPLVR+RR+ D + AD+ AKLE FNP +SVKDR+GVAM++ AE+ GL
Sbjct: 1   MNVYESITETVGRTPLVRIRRMADHSYADVYAKLESFNPLSSVKDRVGVAMIEDAERNGL 60

Query: 61  IKPDTIILEPTSGNTGIALAMVCAARGYRCVLTMPETMSLERRMLLRAYGAELILTPGAD 120
           I P+T I+EPTSGNTGIALA  CA+RGYR +LTMP+TMS+ERR LL+  GAEL+LT GA 
Sbjct: 61  IGPETTIIEPTSGNTGIALAFSCASRGYRLILTMPDTMSVERRRLLKILGAELVLTEGAA 120

Query: 121 GMSGAIAKAEELAKTDQRYFVPQQFENPANPAIHRVTTAEEVWRDTDGKVDIVVAGVGTG 180
           GM GAIA+AE L  +     + QQF NPANP  HR TTA E+W DTDG+VDI +AGVGTG
Sbjct: 121 GMKGAIAEAERLVDSIPHSLMLQQFNNPANPETHRRTTAREIWDDTDGQVDIFIAGVGTG 180

Query: 181 GTITGVAQVIKERKPSARFVAVEPAASPVLSGGQKGPHPIQGIGAGFVPPVLDQDLVDEI 240
           GTITGV  V++E KP    VAVEP  SPV+SGG+ GPH IQGIGAGFVP  LD  ++DEI
Sbjct: 181 GTITGVGSVLRELKPGVHIVAVEPDDSPVISGGEPGPHKIQGIGAGFVPGNLDPSVIDEI 240

Query: 241 ITVGNEDALNVARRLAREEGLLVGISSGAATVAALQVARRPENAGKLIVVVLPDFGERYL 300
           ITV N+DA   AR+LAR+EG+L GISSGAA  AA+ VA RP   GK IVV+LPD GERYL
Sbjct: 241 ITVSNQDAAETARKLARKEGILCGISSGAAMWAAMSVAARPAMEGKNIVVLLPDTGERYL 300

Query: 301 STPLFAD 307
           ST LFAD
Sbjct: 301 STWLFAD 307


Lambda     K      H
   0.318    0.136    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 329
Number of extensions: 9
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 310
Length of database: 308
Length adjustment: 27
Effective length of query: 283
Effective length of database: 281
Effective search space:    79523
Effective search space used:    79523
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 48 (23.1 bits)

Align candidate WP_012506546.1 PAES_RS09995 (cysteine synthase A)
to HMM TIGR01139 (cysK: cysteine synthase A (EC 2.5.1.47))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01139.hmm
# target sequence database:        /tmp/gapView.3571670.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01139  [M=298]
Accession:   TIGR01139
Description: cysK: cysteine synthase A
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   3.2e-143  462.4   0.0   3.6e-143  462.2   0.0    1.0  1  NCBI__GCF_000020625.1:WP_012506546.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000020625.1:WP_012506546.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  462.2   0.0  3.6e-143  3.6e-143       1     298 []       7     305 ..       7     305 .. 0.99

  Alignments for each domain:
  == domain 1  score: 462.2 bits;  conditional E-value: 3.6e-143
                             TIGR01139   1 iseliGntPlvrLn.laeeakaevlvkleslnPsssvkdrialamiedaekegllkkgktiveatsGntGial 72 
                                           i+e++G+tPlvr++ +a++  a+v++kles+nP ssvkdr+++amiedae++gl+ +++ti+e+tsGntGial
  NCBI__GCF_000020625.1:WP_012506546.1   7 ITETVGRTPLVRIRrMADHSYADVYAKLESFNPLSSVKDRVGVAMIEDAERNGLIGPETTIIEPTSGNTGIAL 79 
                                           6899**********8888899**************************************************** PP

                             TIGR01139  73 amvaaargykliltmpetmslerrkllkayGaelvLtdgaegmkgaiekaeelveetpnkylllkqfenpanp 145
                                           a+ +a+rgy+liltmp+tms+err+llk +GaelvLt+ga gmkgai++ae lv++ p++  +l+qf+npanp
  NCBI__GCF_000020625.1:WP_012506546.1  80 AFSCASRGYRLILTMPDTMSVERRRLLKILGAELVLTEGAAGMKGAIAEAERLVDSIPHSL-MLQQFNNPANP 151
                                           **********************************************************766.*********** PP

                             TIGR01139 146 eihrkttapeilkdldgkldafvagvGtGGtitGvgevlkekkpdikvvavePaespvlsggkpgphkiqGig 218
                                           e+hr+tta+ei+ d+dg++d+f+agvGtGGtitGvg+vl+e kp +++vaveP +spv+sgg+pgphkiqGig
  NCBI__GCF_000020625.1:WP_012506546.1 152 ETHRRTTAREIWDDTDGQVDIFIAGVGTGGTITGVGSVLRELKPGVHIVAVEPDDSPVISGGEPGPHKIQGIG 224
                                           ************************************************************************* PP

                             TIGR01139 219 agfiPkvLdkevidevikvsdeeaietarrlakeeGilvGissGaavaaalkvakkle.kdkkivvilpdtge 290
                                           agf+P +Ld +vide+i+vs+++a etar+la++eGil GissGaa++aa+ va +++ ++k+ivv+lpdtge
  NCBI__GCF_000020625.1:WP_012506546.1 225 AGFVPGNLDPSVIDEIITVSNQDAAETARKLARKEGILCGISSGAAMWAAMSVAARPAmEGKNIVVLLPDTGE 297
                                           *********************************************************99************** PP

                             TIGR01139 291 rYlstaLf 298
                                           rYlst Lf
  NCBI__GCF_000020625.1:WP_012506546.1 298 RYLSTWLF 305
                                           *****998 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (298 nodes)
Target sequences:                          1  (308 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 23.58
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis