Alignments for a candidate for ilvD in Acidithiobacillus ferrooxidans ATCC 23270

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvD in Acidithiobacillus ferrooxidans ATCC 23270

Align dihydroxy-acid dehydratase subunit (EC 4.2.1.9) (characterized)
to candidate WP_009560820.1 AFE_RS03125 dihydroxy-acid dehydratase

Query= metacyc::MONOMER-11919
         (549 letters)



>NCBI__GCF_000021485.1:WP_009560820.1
          Length = 558

 Score =  523 bits (1347), Expect = e-153
 Identities = 269/558 (48%), Positives = 372/558 (66%), Gaps = 12/558 (2%)

Query: 1   MKSDTIKRGIQRAPHRSLLARCGLTDDDFEKPFIGIANSYTDIVPGHIHLRELAEAVKEG 60
           M+SD IK+G +R PHR+LL  CG+TD D +KPFIG+ANS+ DI+PGH+HL+E    VKE 
Sbjct: 1   MRSDMIKKGFERTPHRALLKACGVTDQDMDKPFIGVANSFIDIIPGHVHLQEFGRIVKEN 60

Query: 61  VNAAGGVAFEFNTMAICDGIAMNHDGMKYSLASREIVADTVESMAMAHALDGLVLLPTCD 120
           + AAGGV FEFNT+ + DGI M HDGM+YSL SRE++AD++E++  AH LD L+ +P CD
Sbjct: 61  IRAAGGVPFEFNTIGVDDGIIMGHDGMRYSLPSRELIADSIETVVQAHHLDALICIPNCD 120

Query: 121 KIVPGMLMAAARLDIPAIVVTGGPMLPGEFK-GRKVDLINVYEGVGTVSAGEMSEDELEE 179
           KIVPGM+M A R D+P + V+GGPM  G    G  +DL+  +E VG    G++++  L+E
Sbjct: 121 KIVPGMIMGALRCDVPTVFVSGGPMEAGHLSDGTAIDLVTAFEAVGQFKRGQITQIRLKE 180

Query: 180 LERCACPGPRSCAGLFTANTMACLTEALGMSLPGCATAHAVSSRKRQIARLSGKRIVEMV 239
           +E  ACP   SC+G+FTAN+M CL E LG++LPG  T  A ++ +R + R +  R+V + 
Sbjct: 181 IEDKACPTCGSCSGMFTANSMNCLMEVLGIALPGNGTILATAAARRDLVRQAADRVVALA 240

Query: 240 QENLKPTM--IMSQEAFENAVMVDLALGGSTNTTLHIPAIAAEIDGLNINLDLFDELSRV 297
                PTM  ++  +A +NA ++D+A+GGSTNT LH  AIA E  GL+  L   ++L++ 
Sbjct: 241 MAG-GPTMRQLVDFDAIDNAFILDMAMGGSTNTVLHTLAIAREA-GLDYPLARLNDLAQK 298

Query: 298 IPHIASISPAGEHMML-DLDRAGGIPAVL----KTLEDHINRECVTCTGRTVQENIENVK 352
           + ++A ISPA   + + D+ RAGGIPA+L    K   D ++ +  T +G++++E +    
Sbjct: 299 VAYLAKISPALSTVHIEDIGRAGGIPAILHEVHKRNPDVLHLDKPTVSGQSLREIVSAAA 358

Query: 353 VGHRDVIRPLDSPVHSEGGLAILRGNLAPRGSVVKQGAVAEDMMVHEGPAKVFNSEDECM 412
           V    +I     P+   GGL ++ GNLAP G +VK GAV   M    GPAK+F S +   
Sbjct: 359 VKDSRIIHLASDPISPTGGLRVVFGNLAPEGGIVKIGAVLPAMRKFSGPAKIFESMETAA 418

Query: 413 EAIFGGRIDEGDVIVIRYEGPKGGPGMREMLNPTSAIAGMGL-ERVALITDGRFSGGTRG 471
           + I  G +  GDV+VIRYEGPKGGPGM EML PT+ I GMGL E VALITDGRFSG TRG
Sbjct: 419 DGILAGEVKGGDVVVIRYEGPKGGPGMPEMLTPTANIMGMGLGESVALITDGRFSGATRG 478

Query: 472 PCVGHVSPEAMEDGPLAAVNDGDIIRIDIPSRKLEVDLSPREIEERLQSAVKPRRSVKG- 530
            C+GH+SPEA E GP+  + DGD+I +D+ + K+ VDLS  E+ ER +  V   + +K  
Sbjct: 479 ACIGHISPEAAEGGPIGLLKDGDMISLDLDNGKIHVDLSDEELAERRKHWVPIEKPLKSP 538

Query: 531 WLARYRKLAGSADTGAVL 548
           WL RYRKL  +A  GA+L
Sbjct: 539 WLRRYRKLVTNAANGAIL 556


Lambda     K      H
   0.319    0.136    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1021
Number of extensions: 60
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 549
Length of database: 558
Length adjustment: 36
Effective length of query: 513
Effective length of database: 522
Effective search space:   267786
Effective search space used:   267786
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

Align candidate WP_009560820.1 AFE_RS03125 (dihydroxy-acid dehydratase)
to HMM TIGR00110 (ilvD: dihydroxy-acid dehydratase (EC 4.2.1.9))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00110.hmm
# target sequence database:        /tmp/gapView.2138483.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00110  [M=543]
Accession:   TIGR00110
Description: ilvD: dihydroxy-acid dehydratase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   9.7e-235  766.1   2.9   1.1e-234  765.9   2.9    1.0  1  NCBI__GCF_000021485.1:WP_009560820.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000021485.1:WP_009560820.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  765.9   2.9  1.1e-234  1.1e-234       1     542 [.      14     557 ..      14     558 .] 0.98

  Alignments for each domain:
  == domain 1  score: 765.9 bits;  conditional E-value: 1.1e-234
                             TIGR00110   1 aarallkatGlkdedlekPiiavvnsyteivPghvhlkdlaklvkeeieaaGgvakefntiavsDGiamgheG 73 
                                           ++rallka+G++d+d++kP+i+v+ns+++i+Pghvhl++++++vke+i+aaGgv++efnti+v+DGi mgh+G
  NCBI__GCF_000021485.1:WP_009560820.1  14 PHRALLKACGVTDQDMDKPFIGVANSFIDIIPGHVHLQEFGRIVKENIRAAGGVPFEFNTIGVDDGIIMGHDG 86 
                                           69*********************************************************************** PP

                             TIGR00110  74 mkysLpsreiiaDsvetvvkahalDalvvissCDkivPGmlmaalrlniPaivvsGGpmeagktklsekidlv 146
                                           m+ysLpsre+iaDs+etvv+ah+lDal++i++CDkivPGm+m+alr ++P+++vsGGpmeag+++ +  idlv
  NCBI__GCF_000021485.1:WP_009560820.1  87 MRYSLPSRELIADSIETVVQAHHLDALICIPNCDKIVPGMIMGALRCDVPTVFVSGGPMEAGHLSDGTAIDLV 159
                                           ************************************************************************* PP

                             TIGR00110 147 dvfeavgeyaagklseeeleeiersacPtagsCsGlftansmacltealGlslPgsstllatsaekkelakks 219
                                            +feavg+ + g++++ +l+eie+ acPt+gsCsG+ftansm+cl+e+lG++lPg++t+lat a +++l++++
  NCBI__GCF_000021485.1:WP_009560820.1 160 TAFEAVGQFKRGQITQIRLKEIEDKACPTCGSCSGMFTANSMNCLMEVLGIALPGNGTILATAAARRDLVRQA 232
                                           ************************************************************************* PP

                             TIGR00110 220 gkrivelvkkn.ikPrdiltkeafenaitldlalGGstntvLhllaiakeagvklslddfdrlsrkvPllakl 291
                                           + r+v l +    + r+++  +a++na+ ld+a+GGstntvLh+laia+eag+++ l +++ l++kv  lak+
  NCBI__GCF_000021485.1:WP_009560820.1 233 ADRVVALAMAGgPTMRQLVDFDAIDNAFILDMAMGGSTNTVLHTLAIAREAGLDYPLARLNDLAQKVAYLAKI 305
                                           *****99876515789********************************************************* PP

                             TIGR00110 292 kPsgkkv.iedlhraGGvsavlkeldk..egllhkdaltvtGktlaetlekvkvlrvdqdvirsldnpvkkeg 361
                                           +P+ ++v ied+ raGG++a+l+e++k   ++lh d  tv+G++l e+++ + v+  d+ +i+  ++p++ +g
  NCBI__GCF_000021485.1:WP_009560820.1 306 SPALSTVhIEDIGRAGGIPAILHEVHKrnPDVLHLDKPTVSGQSLREIVSAAAVK--DSRIIHLASDPISPTG 376
                                           ***99888*****************9843479**********************9..**************** PP

                             TIGR00110 362 glavLkGnlaeeGavvkiagveedilkfeGpakvfeseeealeailggkvkeGdvvviryeGPkGgPGmremL 434
                                           gl+v++Gnla+eG +vki++v   + kf Gpak+fes e a ++il+g+vk GdvvviryeGPkGgPGm+emL
  NCBI__GCF_000021485.1:WP_009560820.1 377 GLRVVFGNLAPEGGIVKIGAVLPAMRKFSGPAKIFESMETAADGILAGEVKGGDVVVIRYEGPKGGPGMPEML 449
                                           ************************************************************************* PP

                             TIGR00110 435 aPtsalvglGLgkkvaLitDGrfsGgtrGlsiGhvsPeaaegGaialvedGDkikiDienrkldlevseeela 507
                                           +Pt+ ++g+GLg++vaLitDGrfsG+trG +iGh+sPeaaegG+i+l++dGD+i++D++n k+++++s+eela
  NCBI__GCF_000021485.1:WP_009560820.1 450 TPTANIMGMGLGESVALITDGRFSGATRGACIGHISPEAAEGGPIGLLKDGDMISLDLDNGKIHVDLSDEELA 522
                                           ************************************************************************* PP

                             TIGR00110 508 errakakkkearevkgaLakyaklvssadkGavld 542
                                           err+++++ e+  ++ +L++y+klv++a +Ga+l+
  NCBI__GCF_000021485.1:WP_009560820.1 523 ERRKHWVPIEKPLKSPWLRRYRKLVTNAANGAILE 557
                                           **********9999******************986 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (543 nodes)
Target sequences:                          1  (558 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 18.56
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis