Alignments for a candidate for ilvE in Sulfurihydrogenibium azorense Az-Fu1

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvE in Sulfurihydrogenibium azorense Az-Fu1

Align branched-chain-amino-acid transaminase (EC 2.6.1.42) (characterized)
to candidate WP_012674541.1 SULAZ_RS03715 branched-chain amino acid transaminase

Query= BRENDA::F0QW25
         (314 letters)



>NCBI__GCF_000021545.1:WP_012674541.1
          Length = 302

 Score =  306 bits (783), Expect = 5e-88
 Identities = 149/301 (49%), Positives = 210/301 (69%), Gaps = 1/301 (0%)

Query: 14  MKYVWVNGKLIPEKEATIPILTHALHYGTSIFEGIRAYWNSDNNNLYVFRARDHYVRFHD 73
           M  V+   K + E+EA I I T++ HYGT++FEGIRAY+N +N+ +Y    ++HY R   
Sbjct: 1   MAIVYFENKFVEEEEAKISIKTNSFHYGTAVFEGIRAYYNKENDTMYGLFFKEHYERLFK 60

Query: 74  SAKIMSIKVGYSVDELIDATVELLRANDVHEDVYIRPITFVSASTVNLDIRNLDVTTAII 133
           + +I+++++  S+D+L++ T EL++ N+  EDVYIRPI + S   +   +       AI 
Sbjct: 61  NMRILNMEIEESIDQLVEITKELVKRNNHKEDVYIRPIVYFSDLAIGPKLIGYTAKIAIY 120

Query: 134 TVPFGHYLEP-KGIKAKVVSWLRVHNSMFPMKAKVSGIYVNSVIAIIDAKVSGFDEAILL 192
           T+P G Y++  KGIKAKV SW+R++++M P + KV+G YVNS +A  +A +SG +EAI+L
Sbjct: 121 TLPLGDYIDTNKGIKAKVSSWVRLNDNMIPPRLKVTGAYVNSALAKTEALLSGAEEAIVL 180

Query: 193 NRDGYVAEGSGENIFIIKDGILYTPPVYDSILEGITRDTVITIARDLGLTVTEKRITREE 252
           N+DG+V+EGS ENIFI +DG L TPPV D ILEGITR  V+ IA DLG+ V E+ I+R E
Sbjct: 181 NKDGFVSEGSAENIFIARDGKLITPPVSDDILEGITRKAVMDIATDLGIPVIERSISRTE 240

Query: 253 LYTADEVFFTGTAAEVTPVVNIDGRVIGNGEPGPIALKVRSYYMDVVHGRVSKYKNWLTP 312
           LY ADEVFF GT A+++PV+ IDGR IGNG  G I  K++  Y D V G+V KYKNW+  
Sbjct: 241 LYVADEVFFCGTGAQISPVIEIDGRKIGNGTVGDITKKIKDIYFDAVRGKVEKYKNWVVE 300

Query: 313 I 313
           I
Sbjct: 301 I 301


Lambda     K      H
   0.321    0.139    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 318
Number of extensions: 12
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 314
Length of database: 302
Length adjustment: 27
Effective length of query: 287
Effective length of database: 275
Effective search space:    78925
Effective search space used:    78925
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 48 (23.1 bits)

Align candidate WP_012674541.1 SULAZ_RS03715 (branched-chain amino acid transaminase)
to HMM TIGR01122 (ilvE: branched-chain amino acid aminotransferase (EC 2.6.1.42))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01122.hmm
# target sequence database:        /tmp/gapView.1803437.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01122  [M=298]
Accession:   TIGR01122
Description: ilvE_I: branched-chain amino acid aminotransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   1.3e-114  368.3   2.3   1.5e-114  368.2   2.3    1.0  1  NCBI__GCF_000021545.1:WP_012674541.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000021545.1:WP_012674541.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  368.2   2.3  1.5e-114  1.5e-114       1     296 [.       5     299 ..       5     301 .. 0.97

  Alignments for each domain:
  == domain 1  score: 368.2 bits;  conditional E-value: 1.5e-114
                             TIGR01122   1 wldGelvdvedakvhvlthalhYGtgvfeGiRaYetdkglai..frlkehveRlydsakilrleipyskeelv 71 
                                           +++ ++v++e+ak+++ t+++hYGt+vfeGiRaY ++++ ++  +++keh+eRl+++ +il++ei+ s ++lv
  NCBI__GCF_000021545.1:WP_012674541.1   5 YFENKFVEEEEAKISIKTNSFHYGTAVFEGIRAYYNKENDTMygLFFKEHYERLFKNMRILNMEIEESIDQLV 77 
                                           8999******************************9988655522689************************** PP

                             TIGR01122  72 evtkevlrknnlks.aYiRplvyvGaedlglkpk.vdlkveviiaawewgaylgeealekGikvkvssfrraa 142
                                           e+tke++++nn k+ +YiRp+vy  + dl + pk ++++ +++i++ ++g y+++   +kGik+kvss+ r +
  NCBI__GCF_000021545.1:WP_012674541.1  78 EITKELVKRNNHKEdVYIRPIVY--FSDLAIGPKlIGYTAKIAIYTLPLGDYIDT---NKGIKAKVSSWVRLN 145
                                           ***********9988********..8899999999*******************8...7************** PP

                             TIGR01122 143 vnsiptkakaagnYlnsllaksealraGydeailLdeeGyvaeGsGenifivkdgvlltPpvsesiLkgitrd 215
                                           +n+ip+++k++g+Y+ns+lak+eal +G++eai+L+++G+v+eGs enifi +dg+l+tPpvs+ iL+gitr 
  NCBI__GCF_000021545.1:WP_012674541.1 146 DNMIPPRLKVTGAYVNSALAKTEALLSGAEEAIVLNKDGFVSEGSAENIFIARDGKLITPPVSDDILEGITRK 218
                                           ************************************************************************* PP

                             TIGR01122 216 aviklakelgievkeerisreelytaDevfltGtaaevtPirevDgrkigegkrGpvtkklqeaffdlvegkt 288
                                           av+++a++lgi v e++isr+ely+aDevf+ Gt a + P+ e+Dgrkig+g++G++tkk+++ +fd v+gk+
  NCBI__GCF_000021545.1:WP_012674541.1 219 AVMDIATDLGIPVIERSISRTELYVADEVFFCGTGAQISPVIEIDGRKIGNGTVGDITKKIKDIYFDAVRGKV 291
                                           ************************************************************************* PP

                             TIGR01122 289 ekkeewlt 296
                                           ek+++w+ 
  NCBI__GCF_000021545.1:WP_012674541.1 292 EKYKNWVV 299
                                           ******86 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (298 nodes)
Target sequences:                          1  (302 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.02
# Mc/sec: 3.88
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis