Alignments for a candidate for cysK in Persephonella marina EX-H1

GapMind for Amino acid biosynthesis

Alignments for a candidate for cysK in Persephonella marina EX-H1

Align O-phosphoserine sulfhydrylase monomer (EC 2.5.1.47; EC 2.5.1.65) (characterized)
to candidate WP_012676510.1 PERMA_RS07850 cysteine synthase B

Query= metacyc::MONOMER-20568
         (299 letters)



>NCBI__GCF_000021565.1:WP_012676510.1
          Length = 315

 Score =  274 bits (700), Expect = 2e-78
 Identities = 143/298 (47%), Positives = 207/298 (69%), Gaps = 5/298 (1%)

Query: 5   NILETIGNTPLVRINHLNPNP----KVQMYAKLEGFNPTGSVKDRIALKMIEQAEAEGKL 60
           +ILE +GNTPL+++ +  P       V++YAKLEG+NP GSVKDR A +M+ +A   GKL
Sbjct: 17  SILELVGNTPLIKLENSLPEDIKEKDVEIYAKLEGYNPGGSVKDRPATRMLVEAVKSGKL 76

Query: 61  HPGSTIIEATSGNTGIGLAMIGRVKGYNVIIVMSEGVSIERRKMIKAFGAEIILTDKKLG 120
                I++ATSGNTGI LAMIG   G+ V + M   VS ER+K+I+AFGA+I  T+    
Sbjct: 77  TKDKIILDATSGNTGIALAMIGTALGFRVELAMPANVSEERKKIIQAFGAKIHYTNPLES 136

Query: 121 TDGAIRKVAELVKENPGKYFNPNQFSNEYNKIAHYKTTAEEIWAQTKGTVTHFVAAVGTS 180
           TDGAI  V +L+++ P +YF  +Q++N+ N  +HY +TA EIW QT+G +THFVA +GT 
Sbjct: 137 TDGAIIYVRKLIEKYPDRYFYVDQYNNDANWRSHYYSTAVEIWKQTEGRITHFVAGIGTG 196

Query: 181 GTLMGVGKNLREKNPEIKIIEAQP-TKGHYIQGLKSMEEAIVPAIYQADKIDEHILIESE 239
           GT+MG G+ L+  NP+I++I  QP +  H I+GLK +E +I P I+  +++D  + I ++
Sbjct: 197 GTVMGTGRRLKIFNPDIQVIGVQPDSPFHGIEGLKYIETSIKPGIFDENRLDRTMFIGTD 256

Query: 240 EAFAKAREIVAQEGIFIGMSSGAAMLAAQKLAEKIDSGVIVVLFADRGEKYLSTKLFD 297
            ++ +ARE+  +EGIF+G SSGAA  AA +LA +ID GVIV +  D GEKYL+T L++
Sbjct: 257 ISYERARELARKEGIFVGQSSGAAFEAAIQLAREIDEGVIVFICPDGGEKYLTTALWE 314


Lambda     K      H
   0.315    0.133    0.367 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 321
Number of extensions: 17
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 299
Length of database: 315
Length adjustment: 27
Effective length of query: 272
Effective length of database: 288
Effective search space:    78336
Effective search space used:    78336
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 48 (23.1 bits)

Align candidate WP_012676510.1 PERMA_RS07850 (cysteine synthase B)
to HMM TIGR01138 (cysM: cysteine synthase B (EC 2.5.1.47))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01138.hmm
# target sequence database:        /tmp/gapView.1463885.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01138  [M=290]
Accession:   TIGR01138
Description: cysM: cysteine synthase B
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   3.3e-132  426.4   0.0   3.8e-132  426.2   0.0    1.0  1  NCBI__GCF_000021565.1:WP_012676510.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000021565.1:WP_012676510.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  426.2   0.0  3.8e-132  3.8e-132       2     290 .]      18     313 ..      17     313 .. 0.99

  Alignments for each domain:
  == domain 1  score: 426.2 bits;  conditional E-value: 3.8e-132
                             TIGR01138   2 ilklvGntplvrlkrllpe....edsevlvklegnnpaGsvkdrpalsmiveaekrGeikeGdvlieatsGnt 70 
                                           il+lvGntpl++l++ lpe    +d+e+++kleg+np+Gsvkdrpa  m+vea k G++ + +++++atsGnt
  NCBI__GCF_000021565.1:WP_012676510.1  18 ILELVGNTPLIKLENSLPEdikeKDVEIYAKLEGYNPGGSVKDRPATRMLVEAVKSGKLTKDKIILDATSGNT 90 
                                           889**************9989889************************************************* PP

                             TIGR01138  71 GialamvaalkGykvkllmpdnvseerkaalkayGaelilvdkeeGmeGardlarelvrkgeek..lldqfnn 141
                                           Gialam+++++G++v+l+mp+nvseerk++++a+Ga++  ++++e ++Ga+ ++r+l +k++++  ++dq+nn
  NCBI__GCF_000021565.1:WP_012676510.1  91 GIALAMIGTALGFRVELAMPANVSEERKKIIQAFGAKIHYTNPLESTDGAIIYVRKLIEKYPDRyfYVDQYNN 163
                                           ****************************************************************8889***** PP

                             TIGR01138 142 pdnpkahytstGieiwqqtkGrithfvsslGttGtimGvsrflkeqnpavqivGlqpaegsa.ieGlrriese 213
                                            +n+++hy+st +eiw+qt+Grithfv+++Gt+Gt+mG++r+lk++np++q++G+qp  +++ ieGl+ ie++
  NCBI__GCF_000021565.1:WP_012676510.1 164 DANWRSHYYSTAVEIWKQTEGRITHFVAGIGTGGTVMGTGRRLKIFNPDIQVIGVQPDSPFHgIEGLKYIETS 236
                                           ************************************************************************* PP

                             TIGR01138 214 ylpgifdaslvdrvvdveqedaediarelakkegifvGvssGgavaaalrlarelekavvvaiicdrGdryls 286
                                           ++pgifd+ ++dr++++ ++ +++ arela+kegifvG+ssG+a  aa++lare +++v+v i +d+G++yl+
  NCBI__GCF_000021565.1:WP_012676510.1 237 IKPGIFDENRLDRTMFIGTDISYERARELARKEGIFVGQSSGAAFEAAIQLAREIDEGVIVFICPDGGEKYLT 309
                                           ************************************************************************* PP

                             TIGR01138 287 tgvf 290
                                           t+++
  NCBI__GCF_000021565.1:WP_012676510.1 310 TALW 313
                                           *986 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (290 nodes)
Target sequences:                          1  (315 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 18.92
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis