Alignments for a candidate for ilvD in Persephonella marina EX-H1

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvD in Persephonella marina EX-H1

Align dihydroxy-acid dehydratase subunit (EC 4.2.1.9) (characterized)
to candidate WP_012676391.1 PERMA_RS02785 dihydroxy-acid dehydratase

Query= metacyc::MONOMER-11919
         (549 letters)



>NCBI__GCF_000021565.1:WP_012676391.1
          Length = 557

 Score =  588 bits (1515), Expect = e-172
 Identities = 296/555 (53%), Positives = 402/555 (72%), Gaps = 10/555 (1%)

Query: 1   MKSDTIKRGIQRAPHRSLLARCGLTDDDFEKPFIGIANSYTDIVPGHIHLRELAEAVKEG 60
           M+SD +K+G +RAPHRSLL  CGLTD+DF+KPFIGIANSY DI+PGH+HLRE A+ VKE 
Sbjct: 1   MRSDIVKKGFERAPHRSLLRACGLTDEDFKKPFIGIANSYIDIIPGHVHLREFAQIVKEA 60

Query: 61  VNAAGGVAFEFNTMAICDGIAMNHDGMKYSLASREIVADTVESMAMAHALDGLVLLPTCD 120
           V  AGGV FEFN + + DGIAM H GM YSL SRE++AD++E++  AH LD LV +P CD
Sbjct: 61  VREAGGVPFEFNVIGVDDGIAMGHSGMHYSLPSRELIADSIETVVEAHKLDALVCIPNCD 120

Query: 121 KIVPGMLMAAARLDIPAIVVTGGPMLPGEF-KGRKVDLINVYEGVGTVSAGEMSEDELEE 179
           KIVPGM+MAAARL+IP I V+GGPM  G    GR +DL   +E VG VS G M+E+EL  
Sbjct: 121 KIVPGMIMAAARLNIPVIFVSGGPMAAGHLPDGRPIDLATAFEAVGAVSRGLMTENELRV 180

Query: 180 LERCACPGPRSCAGLFTANTMACLTEALGMSLPGCATAHAVSSRKRQIARLSGKRIVEMV 239
           +E  ACP   SC+G+FTAN+M CL+E LG++LPG  +  A   R++++AR +G++IV++V
Sbjct: 181 IEENACPSCGSCSGMFTANSMNCLSEVLGIALPGNGSILATDPRRQELARKAGEQIVKLV 240

Query: 240 QENLKPTMIMSQEAFENAVMVDLALGGSTNTTLHIPAIAAEIDGLNINLDLFDELSRVIP 299
           + +LK   I+++E  ENA  +D+A+GGS+NT LH+ AIA E  G++  ++  D++SR  P
Sbjct: 241 EADLKFRDIVNEETIENAFTLDIAMGGSSNTVLHLLAIANEA-GIDFPVEKIDQISRRTP 299

Query: 300 HIASISPAGEHMMLDLDRAGGIPAVLKTLED----HINRECVTCTGRTVQENIENVKVGH 355
            +  ++PA ++ M DLDRAGGI A+LK L      H++R  V+   +T+ E IE+ ++  
Sbjct: 300 TLCKLAPASQYHMEDLDRAGGISAILKELSKKGLLHLDRPTVSL--KTLGEVIEDAEIKD 357

Query: 356 RDVIRPLDSPVHSEGGLAILRGNLAPRGSVVKQGAVAEDMMVHEGPAKVFNSEDECMEAI 415
            DVIRP+ +P    GGLA+L GN+AP G VVK  AV   +MVH G A  F+SE+E +  I
Sbjct: 358 PDVIRPIHNPYSETGGLAVLFGNIAPYGGVVKAAAVDPKIMVHRGKAVCFDSEEEAIAGI 417

Query: 416 FGGRIDEGDVIVIRYEGPKGGPGMREMLNPTSAIAGMGL-ERVALITDGRFSGGTRGPCV 474
            GG++  GDV+VIRYEGP+GGPGMREML+PTSAI GMGL ++V+LITDGRFSG TRG C+
Sbjct: 418 TGGKVKAGDVVVIRYEGPRGGPGMREMLSPTSAIMGMGLGDKVSLITDGRFSGATRGACI 477

Query: 475 GHVSPEAMEDGPLAAVNDGDIIRIDIPSRKLEVDLSPREIEERLQSAVKPRRSVKG-WLA 533
           GH+SPEA   GP+  + DGD I IDIP+RK+E+ +S  E ++R+++    ++ +K  WL 
Sbjct: 478 GHISPEAAAGGPIGIIKDGDEILIDIPNRKIELLISEEEFKKRMENFKPKKKEIKSRWLR 537

Query: 534 RYRKLAGSADTGAVL 548
           RY +   SA+ GAVL
Sbjct: 538 RYSRFVTSANKGAVL 552


Lambda     K      H
   0.319    0.136    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 984
Number of extensions: 44
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 549
Length of database: 557
Length adjustment: 36
Effective length of query: 513
Effective length of database: 521
Effective search space:   267273
Effective search space used:   267273
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

Align candidate WP_012676391.1 PERMA_RS02785 (dihydroxy-acid dehydratase)
to HMM TIGR00110 (ilvD: dihydroxy-acid dehydratase (EC 4.2.1.9))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00110.hmm
# target sequence database:        /tmp/gapView.507858.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00110  [M=543]
Accession:   TIGR00110
Description: ilvD: dihydroxy-acid dehydratase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   1.2e-256  838.4   1.6   1.4e-256  838.2   1.6    1.0  1  NCBI__GCF_000021565.1:WP_012676391.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000021565.1:WP_012676391.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  838.2   1.6  1.4e-256  1.4e-256       1     541 [.      14     552 ..      14     554 .. 1.00

  Alignments for each domain:
  == domain 1  score: 838.2 bits;  conditional E-value: 1.4e-256
                             TIGR00110   1 aarallkatGlkdedlekPiiavvnsyteivPghvhlkdlaklvkeeieaaGgvakefntiavsDGiamgheG 73 
                                           ++r+ll+a+Gl+ded++kP+i+++nsy++i+Pghvhl+++a++vke++++aGgv++efn+i+v+DGiamgh+G
  NCBI__GCF_000021565.1:WP_012676391.1  14 PHRSLLRACGLTDEDFKKPFIGIANSYIDIIPGHVHLREFAQIVKEAVREAGGVPFEFNVIGVDDGIAMGHSG 86 
                                           69*********************************************************************** PP

                             TIGR00110  74 mkysLpsreiiaDsvetvvkahalDalvvissCDkivPGmlmaalrlniPaivvsGGpmeagktklsekidlv 146
                                           m+ysLpsre+iaDs+etvv+ah+lDalv+i++CDkivPGm+maa+rlniP+i+vsGGpm+ag++  ++ idl 
  NCBI__GCF_000021565.1:WP_012676391.1  87 MHYSLPSRELIADSIETVVEAHKLDALVCIPNCDKIVPGMIMAAARLNIPVIFVSGGPMAAGHLPDGRPIDLA 159
                                           ************************************************************************* PP

                             TIGR00110 147 dvfeavgeyaagklseeeleeiersacPtagsCsGlftansmacltealGlslPgsstllatsaekkelakks 219
                                            +feavg+++ g ++e+el+ ie++acP++gsCsG+ftansm+cl+e+lG++lPg++++lat+ +++ela+k+
  NCBI__GCF_000021565.1:WP_012676391.1 160 TAFEAVGAVSRGLMTENELRVIEENACPSCGSCSGMFTANSMNCLSEVLGIALPGNGSILATDPRRQELARKA 232
                                           ************************************************************************* PP

                             TIGR00110 220 gkrivelvkknikPrdiltkeafenaitldlalGGstntvLhllaiakeagvklslddfdrlsrkvPllaklk 292
                                           g++iv+lv+ ++k rdi+++e++ena+tld+a+GGs+ntvLhllaia+eag+++ ++++d++sr++P+l+kl+
  NCBI__GCF_000021565.1:WP_012676391.1 233 GEQIVKLVEADLKFRDIVNEETIENAFTLDIAMGGSSNTVLHLLAIANEAGIDFPVEKIDQISRRTPTLCKLA 305
                                           ************************************************************************* PP

                             TIGR00110 293 PsgkkviedlhraGGvsavlkeldkegllhkdaltvtGktlaetlekvkvlrvdqdvirsldnpvkkegglav 365
                                           P++++ +edl+raGG+sa+lkel+k+gllh d  tv+ ktl+e++e+++++  d dvir+++np++++gglav
  NCBI__GCF_000021565.1:WP_012676391.1 306 PASQYHMEDLDRAGGISAILKELSKKGLLHLDRPTVSLKTLGEVIEDAEIK--DPDVIRPIHNPYSETGGLAV 376
                                           ***************************************************..******************** PP

                             TIGR00110 366 LkGnlaeeGavvkiagveedilkfeGpakvfeseeealeailggkvkeGdvvviryeGPkGgPGmremLaPts 438
                                           L+Gn+a+ G vvk+a+v+ +i++++G+a  f+seeea+++i ggkvk+GdvvviryeGP+GgPGmremL Pts
  NCBI__GCF_000021565.1:WP_012676391.1 377 LFGNIAPYGGVVKAAAVDPKIMVHRGKAVCFDSEEEAIAGITGGKVKAGDVVVIRYEGPRGGPGMREMLSPTS 449
                                           ************************************************************************* PP

                             TIGR00110 439 alvglGLgkkvaLitDGrfsGgtrGlsiGhvsPeaaegGaialvedGDkikiDienrkldlevseeelaerra 511
                                           a++g+GLg+kv+LitDGrfsG+trG +iGh+sPeaa+gG+i++++dGD+i iDi+nrk++l +seee+++r +
  NCBI__GCF_000021565.1:WP_012676391.1 450 AIMGMGLGDKVSLITDGRFSGATRGACIGHISPEAAAGGPIGIIKDGDEILIDIPNRKIELLISEEEFKKRME 522
                                           ************************************************************************* PP

                             TIGR00110 512 kakkkearevkgaLakyaklvssadkGavl 541
                                           + k+k+++ ++ +L++y+++v+sa+kGavl
  NCBI__GCF_000021565.1:WP_012676391.1 523 NFKPKKKEIKSRWLRRYSRFVTSANKGAVL 552
                                           ******99999******************8 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (543 nodes)
Target sequences:                          1  (557 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 19.04
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis