Alignments for a candidate for asnA in Methylobacterium nodulans ORS 2060

GapMind for Amino acid biosynthesis

Alignments for a candidate for asnA in Methylobacterium nodulans ORS 2060

Align asparagine synthetase B, glutamine-hydrolyzing; EC 6.3.5.4 (characterized)
to candidate WP_012631017.1 MNOD_RS38500 asparagine synthase (glutamine-hydrolyzing)

Query= CharProtDB::CH_002444
         (554 letters)



>NCBI__GCF_000022085.1:WP_012631017.1
          Length = 654

 Score =  141 bits (355), Expect = 9e-38
 Identities = 116/372 (31%), Positives = 172/372 (46%), Gaps = 53/372 (14%)

Query: 28  MRHRGPDWSGIYASDNA--ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALR 85
           +RHRGPD   I+    A   L H RLSI+ ++ G QP+ +Q     L VNGE Y+ + +R
Sbjct: 27  IRHRGPDERDIWLDPEAGVALGHTRLSIIGLSNGRQPIASQDGALQLIVNGEFYDFERIR 86

Query: 86  AEYGDR-YQFQTGSDCEVILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGII 144
           AE   R   F+T SD E+ L LY+E G   L  L+G FA  LYD+ +   ++ RD  GI 
Sbjct: 87  AELQARGCVFRTQSDSEIALHLYREFGTAGLGRLRGEFALVLYDAVERQLVVMRDRFGIK 146

Query: 145 PLYMGYDEHGQLYVASEMKALV-----------------------PVCRTIKEFPAGSYL 181
           PL++  +  G L+V SE+KALV                        + + I+    G  L
Sbjct: 147 PLFVA-EHDGALWVGSEIKALVAAGVPAFWDLDAYVSRGFYLGNRTLFQGIRSLAPGHLL 205

Query: 182 WSQDGEI--RSYYHRDWFDYDAVKDNVTDKNE----LRQALEDSVKSHLMSDVPYGVLLS 235
            +  G I  R+Y+  D+    A+     D+ E    LR  + ++V   L +DVP  V LS
Sbjct: 206 IASPGHIRERAYWDLDFPAAAALDSAAIDEAEAVEGLRAEILEAVGLRLRADVPIAVYLS 265

Query: 236 GGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLPGSPDL---KAAQEVANHLG 292
           GG+DSS +     +                  L +F +      D    + A E A+  G
Sbjct: 266 GGIDSSAMLGCATRLRG-------------TPLDAFTLSFTDDGDYDERRFAAEAASFNG 312

Query: 293 TVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRA-STPMYLMSRKIKAMGIKMVLSGEGS 351
              H I  T  +  D   + ++H E   V    A     Y +SR +     K+V++GEG+
Sbjct: 313 ARFHPIPVTQDDLADDFEEALWHNE---VPFFNAHGVAKYRLSRVVAQARFKVVITGEGA 369

Query: 352 DEVFGGYLYFHK 363
           DE+F GY +F +
Sbjct: 370 DEIFAGYPHFRR 381



 Score = 37.7 bits (86), Expect = 1e-06
 Identities = 26/87 (29%), Positives = 41/87 (47%), Gaps = 3/87 (3%)

Query: 398 VEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQKEQFSDGV 457
           +E RVP LD    + A R+    K+   G  EKH+ RE    +LP ++  R+K  F    
Sbjct: 509 IEGRVPLLDHHVAEYAARLPVWLKI--RGATEKHVFREAMRPFLPEALYRRKKHYFRAPP 566

Query: 458 GYS-WIDTLKEVAAQQVSDQQLETARF 483
             S   + L ++ A  ++ + LE   F
Sbjct: 567 AMSAQRNRLAQLVADTLASRHLEDLPF 593


Lambda     K      H
   0.319    0.135    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 813
Number of extensions: 50
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 554
Length of database: 654
Length adjustment: 37
Effective length of query: 517
Effective length of database: 617
Effective search space:   318989
Effective search space used:   318989
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis