Alignments for a candidate for argC in Rhizobium leguminosarum bv. trifolii WSM1325

GapMind for Amino acid biosynthesis

Alignments for a candidate for argC in Rhizobium leguminosarum bv. trifolii WSM1325

Align N-acetylglutamylphosphate reductase (EC 1.2.1.38) (characterized)
to candidate WP_012756931.1 RLEG_RS06480 N-acetyl-gamma-glutamyl-phosphate reductase

Query= reanno::Smeli:SMc01801
         (310 letters)



>NCBI__GCF_000023185.1:WP_012756931.1
          Length = 310

 Score =  493 bits (1268), Expect = e-144
 Identities = 246/310 (79%), Positives = 267/310 (86%)

Query: 1   MKPKIFIDGEHGTTGLQIRVRMAGRTDLELLSIPEAERRNAAMREDLLNSADIAILCLPD 60
           M PKIFIDGEHGTTGLQIR RMAGR D+ELLSIPEAERRNAAMRED+LN ADIAILCLPD
Sbjct: 1   MAPKIFIDGEHGTTGLQIRTRMAGRRDVELLSIPEAERRNAAMREDMLNGADIAILCLPD 60

Query: 61  DASREAVAMVAGNNRVRIIDTSTAHRVAPDWAYGFAEMDKAQPQRIRDARHVANPGCYPT 120
           DAS+EAV MV+ NN VR+IDTSTA RV P WAYGFAEMDK Q  +I  AR VANPGCYPT
Sbjct: 61  DASKEAVQMVSANNNVRVIDTSTAFRVNPGWAYGFAEMDKQQADKIAAARFVANPGCYPT 120

Query: 121 GAIALIRPLRQAGILPDGYPVTVNAVSGYTGGGKQMIAQMEDDQNPDHIGAPHFLYGLTL 180
           GAI LIRPLR AGILPDGYP+TVNAVSGYTGGGKQMIAQME+  +PD I APHFLYGL L
Sbjct: 121 GAIGLIRPLRAAGILPDGYPITVNAVSGYTGGGKQMIAQMENPDHPDAITAPHFLYGLPL 180

Query: 181 KHKHVPEMKMHGLLERAPVFSPSVGKFAQGMIVQVPLYLEDLAAGATLETIHRALVDHYA 240
            HKHVPEM +HGLL+RAP+FSPSVGKFAQGMIVQVPL+L+DLA G T+E IH ALV HYA
Sbjct: 181 THKHVPEMTVHGLLDRAPIFSPSVGKFAQGMIVQVPLHLDDLAEGTTMENIHAALVAHYA 240

Query: 241 GQSIVEVVPLDESAKLARIDATELAGSDAMKLFVFGTKGGAHVNLVALLDNLGKGASGAA 300
           GQ IV VVPL ES  L R++A +L G D MKLFVFGT G + VNLVALLDNLGKGASGAA
Sbjct: 241 GQDIVSVVPLAESKALPRVNAIDLEGKDTMKLFVFGTAGASQVNLVALLDNLGKGASGAA 300

Query: 301 VQNMDLMLSA 310
           VQNMDLML++
Sbjct: 301 VQNMDLMLAS 310


Lambda     K      H
   0.319    0.136    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 396
Number of extensions: 13
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 310
Length of database: 310
Length adjustment: 27
Effective length of query: 283
Effective length of database: 283
Effective search space:    80089
Effective search space used:    80089
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 48 (23.1 bits)

Align candidate WP_012756931.1 RLEG_RS06480 (N-acetyl-gamma-glutamyl-phosphate reductase)
to HMM TIGR01851 (argC: N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01851.hmm
# target sequence database:        /tmp/gapView.1123266.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01851  [M=310]
Accession:   TIGR01851
Description: argC_other: N-acetyl-gamma-glutamyl-phosphate reductase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   2.4e-130  420.3   0.1   2.7e-130  420.2   0.1    1.0  1  NCBI__GCF_000023185.1:WP_012756931.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000023185.1:WP_012756931.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  420.2   0.1  2.7e-130  2.7e-130       1     308 [.       2     308 ..       2     310 .] 0.98

  Alignments for each domain:
  == domain 1  score: 420.2 bits;  conditional E-value: 2.7e-130
                             TIGR01851   1 kpkvfidGeaGttGlqirerlaerddlellsidedkrkdaderakllnaadvailclpddaareavslvd.np 72 
                                           +pk+fidGe GttGlqir r+a+r d+ellsi e  r++a+ r ++ln ad+ailclpdda++eav +v+ n+
  NCBI__GCF_000023185.1:WP_012756931.1   2 APKIFIDGEHGTTGLQIRTRMAGRRDVELLSIPEAERRNAAMREDMLNGADIAILCLPDDASKEAVQMVSaNN 74 
                                           69*****************************************************************997478 PP

                             TIGR01851  73 ntkildastayrtaedwvyGfpelaaeqrekianakrvanPGcyatgaiallrPlveaGilPadfPvtinavs 145
                                           n++++d+sta+r++++w+yGf+e++ +q +kia+a++vanPGcy+tgai l+rPl +aGilP  +P+t+navs
  NCBI__GCF_000023185.1:WP_012756931.1  75 NVRVIDTSTAFRVNPGWAYGFAEMDKQQADKIAAARFVANPGCYPTGAIGLIRPLRAAGILPDGYPITVNAVS 147
                                           89*********************************************************************** PP

                             TIGR01851 146 GysGGGkaliakyeeesaddskkaafiiyglalehkhlpemrkhsglaskPiftPavGdfaqGllveiplhla 218
                                           Gy+GGGk++ia+ e+    d+ +a+++ ygl l+hkh+pem++h+ l + Pif P+vG+faqG++v++plhl 
  NCBI__GCF_000023185.1:WP_012756931.1 148 GYTGGGKQMIAQMENPDHPDAITAPHFLYGLPLTHKHVPEMTVHGLLDRAPIFSPSVGKFAQGMIVQVPLHLD 220
                                           **************99999999*************************************************** PP

                             TIGR01851 219 eldskvsaedihkalaeyykGekfvkvaelddaellddtildaqglngtnrlelfvfgsddgerallvarldn 291
                                           +l++ ++ e+ih+al+ +y+G++ v v++l+++++l    ++a  l+g ++++lfvfg++ + ++ lva+ldn
  NCBI__GCF_000023185.1:WP_012756931.1 221 DLAEGTTMENIHAALVAHYAGQDIVSVVPLAESKAL--PRVNAIDLEGKDTMKLFVFGTAGASQVNLVALLDN 291
                                           ************************************..6677778889999********************** PP

                             TIGR01851 292 lGkGasGaavqnlnial 308
                                           lGkGasGaavqn++++l
  NCBI__GCF_000023185.1:WP_012756931.1 292 LGKGASGAAVQNMDLML 308
                                           ****************9 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (310 nodes)
Target sequences:                          1  (310 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 22.50
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the original publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis