Alignments for a candidate for PPYAT in Rhizobium leguminosarum bv. trifolii WSM1325

GapMind for Amino acid biosynthesis

Alignments for a candidate for PPYAT in Rhizobium leguminosarum bv. trifolii WSM1325

Align branched-chain-amino-acid transaminase (EC 2.6.1.42) (characterized)
to candidate WP_012758180.1 RLEG_RS13530 PLP-dependent aminotransferase family protein

Query= BRENDA::A0A060PQX5
         (417 letters)



>NCBI__GCF_000023185.1:WP_012758180.1
          Length = 405

 Score =  301 bits (770), Expect = 3e-86
 Identities = 169/411 (41%), Positives = 239/411 (58%), Gaps = 20/411 (4%)

Query: 16  LDYEKYFSKKALGMKASEVRELLKLVESSDVISLAGGLPAPETFPVEIIAEITKEVLEKH 75
           L+++  F+ ++  M+ASE+RELLKL++  D+IS AGG+P P  FP +   +   ++    
Sbjct: 2   LNWDTMFASRSSRMRASEIRELLKLLDRPDIISFAGGIPDPALFPDQEFKQAYADIFAAA 61

Query: 76  AAQALQYGTTKGFTPLRLALAEWM---RKRYDIPISKVDIMITSGSQQALDLIGRVFINP 132
              ALQY  ++G+ PLR    EW+        IP    ++ I SGSQQ LD +G++F++P
Sbjct: 62  VNSALQYSVSEGYKPLR----EWLVGQMAALGIPCELDNVFIVSGSQQGLDYLGKLFLSP 117

Query: 133 GDIVVVEAPTYLAALQAFKYYEPEFVQIPLDDEGMRVDLLEEKLQELEKEGKKVKLVYTI 192
            D  +V  PTYL ALQAF  YEP + Q  L   G R    +         G KVK  Y  
Sbjct: 118 DDTALVTWPTYLGALQAFNAYEPAYDQ--LTPNGNRTP--DSYRSAASTAGGKVKFAYLS 173

Query: 193 PTFQNPAGVTMSEKRRKRLLELASEYDFLIVEDNPYGELRYSGEPVKPIKAWD------- 245
             F NP G T+    RK++L LA + D  ++ED  Y  LRY G+P+ PI A +       
Sbjct: 174 ADFSNPTGETVDLDGRKKVLALAEDLDIAVIEDAAYQSLRYDGDPIPPILALEIAEKGHI 233

Query: 246 DEGRVMYLGTFSKILAPGFRIGWIAAEPHLIRKLEIAKQSVDLCTNPFSQVIAWKYVEGG 305
           ++ R +Y G+FSK LAPG R+G+I A   +IRKL + KQ+ DL ++  +Q+      E G
Sbjct: 234 NDTRTIYCGSFSKTLAPGLRVGFIVANAPVIRKLVLMKQAADLHSSTINQMAISDVAERG 293

Query: 306 HLDNHIPNIIEFYKPRRDAMLKALEEFMPEGVRWTKPEGGMFVWVTLPEGID-TKLMLEK 364
             D  +  I   Y  RRD ML AL+++MPEG  WTKPEGGMF+W+TLPEG+D  KL+ + 
Sbjct: 294 -FDAQVAKIKAAYSQRRDCMLTALDKYMPEGTSWTKPEGGMFIWITLPEGMDGAKLLAKS 352

Query: 365 AVAKGVAYVPGEAFFAHRDVKNTMRLNFTYVPEEKIREGIKRLAETIKEEM 415
                VA+VPG+AFFA     NT+RL+F+   E+ I +GI RL+  I  E+
Sbjct: 353 LETAKVAFVPGKAFFADGSGANTIRLSFSCANEQMIEDGIGRLSALISAEI 403


Lambda     K      H
   0.318    0.137    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 399
Number of extensions: 22
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 417
Length of database: 405
Length adjustment: 31
Effective length of query: 386
Effective length of database: 374
Effective search space:   144364
Effective search space used:   144364
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis