Alignments for a candidate for glnA in Acidimicrobium ferrooxidans DSM 10331

GapMind for Amino acid biosynthesis

Alignments for a candidate for glnA in Acidimicrobium ferrooxidans DSM 10331

Align glutamine synthetase, type I (characterized)
to candidate WP_015797717.1 AFER_RS01260 type I glutamate--ammonia ligase

Query= metacyc::SYNPCC7942_2156-MONOMER
         (473 letters)



>NCBI__GCF_000023265.1:WP_015797717.1
          Length = 475

 Score =  595 bits (1535), Expect = e-175
 Identities = 285/472 (60%), Positives = 355/472 (75%), Gaps = 1/472 (0%)

Query: 2   PETAQEVLQLIQEKGIQLVDLKFIDMPGIWQHLTLHVSQIDENSFVEGVPFDGSSIRGWK 61
           P    EV+ + +E+G+++VDL+F D+PG  QH ++ + Q+ E+SF EG  FDGSSIRG+K
Sbjct: 3   PRAPHEVIAMAEEQGVEMVDLRFCDLPGTMQHFSVPIRQLTEDSFEEGFGFDGSSIRGFK 62

Query: 62  AINESDMTMVPDPTTAWIDPFMKEPTLSMICTIYEPRTNTPYERCPRAIAQKAVDYLKAS 121
            INESDM + PDP +A++DPF K  TL + C IY+P T  PY+R PR +A  A ++L+AS
Sbjct: 63  TINESDMILKPDPRSAYLDPFRKHRTLVLNCFIYDPVTGQPYDRDPRRVAAAAEEHLRAS 122

Query: 122 GIGDTAYFGPEAEFFIFDDVRFDQAENKSYYYVDSIEGRWNSGREEEGGNLGYKPRYKEG 181
           GI DTAYFGPEAEFFIFDDVR+ Q E+ + Y VDSIEG WN+ R E+G NLG K  +K G
Sbjct: 123 GIADTAYFGPEAEFFIFDDVRYHQDEHSASYVVDSIEGAWNTDRNEQGSNLGGKIPFKRG 182

Query: 182 YFPVAPTDTSQDMRSEMLLTMADCGVPIEKHHHEVATGGQCELGFRFGKLIEAADWLMTY 241
           YFPV P D  QD+RSEM+LTM   G+ IE  HHEVAT GQ E+  R+  L+  AD LM Y
Sbjct: 183 YFPVPPMDKFQDLRSEMVLTMQALGIEIEVQHHEVATAGQAEIDMRYDTLLAMADKLMLY 242

Query: 242 KYVIKNVGRRYGKTITFMPKPLFNDNGSGMHTHQSIWNDGQPLF-AGDQYAGLSQMALWY 300
           KYV+K+V ++ G T TFMPKPLF DNGSGMHTHQS+W DG+PLF     YA LS++AL Y
Sbjct: 243 KYVVKSVAQQGGYTATFMPKPLFQDNGSGMHTHQSLWRDGEPLFYESGGYADLSELALHY 302

Query: 301 IGGILKHAPALLALTNPTTNSYKRLVPGFEAPVNLAYSQGNRSASVRIPLSGTNPKAKRL 360
           IGGIL HAP++LA   PTTNSYKRLVPG+EAPVNL +SQ NRSA+ RIP+   +PKAKR+
Sbjct: 303 IGGILAHAPSILAFAAPTTNSYKRLVPGYEAPVNLVFSQRNRSAACRIPMYSLSPKAKRV 362

Query: 361 EFRCPDATSNPYLAFAAMLCAGIDGIKNQIDPGEPLDVDIYDLSPEELAKIPSTPGSLKD 420
           EFRCPDA +NPYLAF+AML AG+DGI+N+I+P  P D D++DL P+E   +P+ P SL+ 
Sbjct: 363 EFRCPDAMANPYLAFSAMLLAGLDGIRNKIEPAGPDDRDLFDLPPDEARALPTVPSSLEG 422

Query: 421 ALKALEADHDFLTVGGVFTEDFIQNWIEYKLDNEVIPISLRPHPYEFALYYD 472
           +L ALEADHD+L  GGVF++D I  WI YK   EV  I LRPHPYEFALYYD
Sbjct: 423 SLAALEADHDYLLEGGVFSQDLIDTWIAYKRAEEVDAIRLRPHPYEFALYYD 474


Lambda     K      H
   0.319    0.138    0.428 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 766
Number of extensions: 30
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 473
Length of database: 475
Length adjustment: 33
Effective length of query: 440
Effective length of database: 442
Effective search space:   194480
Effective search space used:   194480
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

Align candidate WP_015797717.1 AFER_RS01260 (type I glutamate--ammonia ligase)
to HMM TIGR00653 (glnA: glutamine synthetase, type I (EC 6.3.1.2))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00653.hmm
# target sequence database:        /tmp/gapView.2066518.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00653  [M=462]
Accession:   TIGR00653
Description: GlnA: glutamine synthetase, type I
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   2.8e-204  665.0   0.0   3.1e-204  664.8   0.0    1.0  1  NCBI__GCF_000023265.1:WP_015797717.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000023265.1:WP_015797717.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  664.8   0.0  3.1e-204  3.1e-204       2     462 .]       8     474 ..       7     474 .. 0.99

  Alignments for each domain:
  == domain 1  score: 664.8 bits;  conditional E-value: 3.1e-204
                             TIGR00653   2 evlkllkeenvkfvdlrfvDikGklkkveipvseleeealeegiaFDgssveGfksieesDlllkpdpetlvi 74 
                                           ev+++++e+ v++vdlrf+D+ G+++++++p+++l+e+ +eeg  FDgss++Gfk+i+esD++lkpdp ++  
  NCBI__GCF_000023265.1:WP_015797717.1   8 EVIAMAEEQGVEMVDLRFCDLPGTMQHFSVPIRQLTEDSFEEGFGFDGSSIRGFKTINESDMILKPDPRSAYL 80 
                                           688999******************************************************************* PP

                             TIGR00653  75 vPfraekvlrvicdvyepvtkepyerdpRsiakraeeelk.tklgdevyfGpEaEFflfdkvefkeasnssfl 146
                                           +Pfr++++l+++c +y+pvt++py+rdpR +a  aee+l+ ++++d++yfGpEaEFf+fd+v+ ++ ++s+ +
  NCBI__GCF_000023265.1:WP_015797717.1  81 DPFRKHRTLVLNCFIYDPVTGQPYDRDPRRVAAAAEEHLRaSGIADTAYFGPEAEFFIFDDVRYHQDEHSASY 153
                                           ****************************************9******************************** PP

                             TIGR00653 147 evdseegewn..reveegnkgykikkkggYfdvepvDkakdirrelvlaleelglevevsHHEvata.qaEid 216
                                            vds+eg+wn  r+++++n g ki  k+gYf+v+p+Dk +d+r+e+vl++++lg+e+ev+HHEvata qaEid
  NCBI__GCF_000023265.1:WP_015797717.1 154 VVDSIEGAWNtdRNEQGSNLGGKIPFKRGYFPVPPMDKFQDLRSEMVLTMQALGIEIEVQHHEVATAgQAEID 226
                                           **********97777888******************************************************* PP

                             TIGR00653 217 ikfdklvkaaDeivlyKyvvknvakkhGktatFmpKplfgdngsGmHvhlslwkdgenlfageegyagLseta 289
                                           +++d+l+ +aD+++lyKyvvk va++ G+tatFmpKplf+dngsGmH+h+slw+dge+lf+++ gya+Lse a
  NCBI__GCF_000023265.1:WP_015797717.1 227 MRYDTLLAMADKLMLYKYVVKSVAQQGGYTATFMPKPLFQDNGSGMHTHQSLWRDGEPLFYESGGYADLSELA 299
                                           ************************************************************************* PP

                             TIGR00653 290 lyyigGilkHakalaAltnptvnsYkRLvpGyEAPvylaysaknRsaliRiPasa.npkakRiEvRspDpsan 361
                                           l+yigGil Ha+++ A+++pt+nsYkRLvpGyEAPv+l++s++nRsa+ RiP+++ +pkakR+E+R+pD+ an
  NCBI__GCF_000023265.1:WP_015797717.1 300 LHYIGGILAHAPSILAFAAPTTNSYKRLVPGYEAPVNLVFSQRNRSAACRIPMYSlSPKAKRVEFRCPDAMAN 372
                                           ************************************************************************* PP

                             TIGR00653 362 pYLafaallmAgldGiknkidpgepldknlyelseeelkelGieqlpesLeealdelesdk..evlkevlgee 432
                                           pYLaf+a+l AgldGi+nki+p  p d++l++l+++e++   ++++p+sLe +l +le+d+  +++++v++++
  NCBI__GCF_000023265.1:WP_015797717.1 373 PYLAFSAMLLAGLDGIRNKIEPAGPDDRDLFDLPPDEARA--LPTVPSSLEGSLAALEADHdyLLEGGVFSQD 443
                                           ****************************************..******************988899******* PP

                             TIGR00653 433 lieafielkr.kEveelrlkvhpvElekyld 462
                                           li+++i++kr +Ev+ +rl++hp+E++ y+d
  NCBI__GCF_000023265.1:WP_015797717.1 444 LIDTWIAYKRaEEVDAIRLRPHPYEFALYYD 474
                                           **********999****************96 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (462 nodes)
Target sequences:                          1  (475 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 22.03
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis