Alignments for a candidate for ilvD in Nitratifractor salsuginis DSM 16511

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvD in Nitratifractor salsuginis DSM 16511

Align dihydroxy-acid dehydratase subunit (EC 4.2.1.9) (characterized)
to candidate WP_013553008.1 NITSA_RS00210 dihydroxy-acid dehydratase

Query= metacyc::MONOMER-11919
         (549 letters)



>NCBI__GCF_000186245.1:WP_013553008.1
          Length = 562

 Score =  509 bits (1311), Expect = e-148
 Identities = 276/563 (49%), Positives = 377/563 (66%), Gaps = 19/563 (3%)

Query: 1   MKSDTIKRGIQRAPHRSLLARCGLTDDDFEKPFIGIANSYTDIVPGHIHLRELAEAVKEG 60
           M+SD IK+G  RAPHRSLL   GL D+DF+KPFIG+ANSYTD+VPGH  L ++A+ +KE 
Sbjct: 1   MRSDEIKKGYDRAPHRSLLRAIGLKDEDFDKPFIGVANSYTDMVPGHFFLNKVAQIIKEE 60

Query: 61  VNAAGGVAFEFNTMAICDGIAMNHDGMKYSLASREIVADTVESMAMAHALDGLVLLPTCD 120
           + A G V FEFNT+ + DGIAM HDGM YSL SREI+A+ VE+M  AH LD ++ +P CD
Sbjct: 61  IRAQGCVPFEFNTIGVDDGIAMGHDGMLYSLPSREIIANAVETMMNAHKLDAMIAIPNCD 120

Query: 121 KIVPGMLMAAARLDIPAIVVTGGPMLPGEFK-GRKVDLINVYEGVGTVSAGEMSEDELEE 179
           KIVPGM+M A R+++P + V+GGPM  G  K G  +DL  V+EGVG   AGE+ E  L E
Sbjct: 121 KIVPGMIMGALRVNVPTVFVSGGPMAAGHLKDGTPIDLATVFEGVGQYEAGEIDEQTLIE 180

Query: 180 LERCACPGPRSCAGLFTANTMACLTEALGMSLPGCATAHAVSSRKRQIARLSGKRIVEMV 239
           +E  ACPG  SC+G+FTAN+M  L EA+G++LPG  T  A++  + ++ R + +RI E+ 
Sbjct: 181 MECNACPGGGSCSGMFTANSMNTLMEAMGIALPGNGTIPALTPEREELYRKAARRICEIA 240

Query: 240 Q------ENLKPTMIMSQEAFENAVMVDLALGGSTNTTLHIPAIAAEIDGLNINLDLFDE 293
           +      E  K   I++++A  NA  VD+A+GGS+NT LH+ AIA E  G++ N+   +E
Sbjct: 241 KAEQSEYERFKIRNILNEKAVRNAFAVDMAMGGSSNTVLHMLAIADEA-GVDFNIGDINE 299

Query: 294 LSRVIPHIASISPAGEHM-MLDLDRAGGIPAV---LKTLEDHINRECVTCTGRTVQENIE 349
           +S+ + HIA ISP+   + M D+++AGG+ AV   +K   D + ++ +T TG T+ E I 
Sbjct: 300 ISKHVAHIAKISPSLTTVHMEDINKAGGVSAVMHEMKRRRDSVLQDNLTVTGETLFERIA 359

Query: 350 NVKVGHRDVIRPLDSPVHSEGGLAILRGNLAPRGSVVKQGAVAEDMMVHEGPAKVFNSED 409
           + K+   ++I  +++P    GGLAIL GNLA +G+V+K   +  +     G A  F+S+ 
Sbjct: 360 DAKILDSEIIHSIENPYSEVGGLAILFGNLAEQGAVIKTAGIVGERS-FTGKAICFDSQQ 418

Query: 410 ECMEAIFGGRIDEGDVIVIRYEGPKGGPGMREMLNPTSAIAGMGL-ERVALITDGRFSGG 468
           E +E I  G++  G V+VIRYEGPKGGPGM+EML PTS I GMGL ++VALITDGRFSG 
Sbjct: 419 EAIEGITSGKVKPGHVVVIRYEGPKGGPGMQEMLAPTSLIMGMGLGDKVALITDGRFSGA 478

Query: 469 TRGPCVGHVSPEAMEDGPLAAVNDGDIIRIDIPSRKLEVDLSPREIEERLQS---AVKPR 525
           TRG  +GHVSPEA E G +  + DGD I +D+    LEV LS  EIE+R +     VKP 
Sbjct: 479 TRGASIGHVSPEAAEGGMIGLLKDGDEIHLDVDKYILEVRLSDEEIEKRRKEFTPKVKPL 538

Query: 526 RSVKGWLARYRKLAGSADTGAVL 548
            S   WL +YR L  +A  GAVL
Sbjct: 539 SS--RWLRQYRALVTNASRGAVL 559


Lambda     K      H
   0.319    0.136    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 891
Number of extensions: 43
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 549
Length of database: 562
Length adjustment: 36
Effective length of query: 513
Effective length of database: 526
Effective search space:   269838
Effective search space used:   269838
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

Align candidate WP_013553008.1 NITSA_RS00210 (dihydroxy-acid dehydratase)
to HMM TIGR00110 (ilvD: dihydroxy-acid dehydratase (EC 4.2.1.9))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00110.hmm
# target sequence database:        /tmp/gapView.361321.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00110  [M=543]
Accession:   TIGR00110
Description: ilvD: dihydroxy-acid dehydratase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   1.1e-233  762.6   8.2   1.3e-233  762.4   8.2    1.0  1  NCBI__GCF_000186245.1:WP_013553008.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000186245.1:WP_013553008.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  762.4   8.2  1.3e-233  1.3e-233       1     541 [.      14     559 ..      14     561 .. 0.98

  Alignments for each domain:
  == domain 1  score: 762.4 bits;  conditional E-value: 1.3e-233
                             TIGR00110   1 aarallkatGlkdedlekPiiavvnsyteivPghvhlkdlaklvkeeieaaGgvakefntiavsDGiamgheG 73 
                                           ++r+ll+a+Glkded++kP+i+v+nsyt++vPgh  l+++a+++keei+a G v++efnti+v+DGiamgh+G
  NCBI__GCF_000186245.1:WP_013553008.1  14 PHRSLLRAIGLKDEDFDKPFIGVANSYTDMVPGHFFLNKVAQIIKEEIRAQGCVPFEFNTIGVDDGIAMGHDG 86 
                                           69*********************************************************************** PP

                             TIGR00110  74 mkysLpsreiiaDsvetvvkahalDalvvissCDkivPGmlmaalrlniPaivvsGGpmeagktklsekidlv 146
                                           m+ysLpsreiia++vet+++ah+lDa+++i++CDkivPGm+m+alr+n+P+++vsGGpm+ag++k +  idl 
  NCBI__GCF_000186245.1:WP_013553008.1  87 MLYSLPSREIIANAVETMMNAHKLDAMIAIPNCDKIVPGMIMGALRVNVPTVFVSGGPMAAGHLKDGTPIDLA 159
                                           ************************************************************************* PP

                             TIGR00110 147 dvfeavgeyaagklseeeleeiersacPtagsCsGlftansmacltealGlslPgsstllatsaekkelakks 219
                                            vfe+vg+y+ag+++e++l e+e +acP+ gsCsG+ftansm++l+ea+G++lPg++t++a + e++el +k+
  NCBI__GCF_000186245.1:WP_013553008.1 160 TVFEGVGQYEAGEIDEQTLIEMECNACPGGGSCSGMFTANSMNTLMEAMGIALPGNGTIPALTPEREELYRKA 232
                                           ************************************************************************* PP

                             TIGR00110 220 gkrivelvkknik......PrdiltkeafenaitldlalGGstntvLhllaiakeagvklslddfdrlsrkvP 286
                                           ++ri e+ k +         r+il+++a+ na+++d+a+GGs+ntvLh+laia+eagv++++ d++++s++v 
  NCBI__GCF_000186245.1:WP_013553008.1 233 ARRICEIAKAEQSeyerfkIRNILNEKAVRNAFAVDMAMGGSSNTVLHMLAIADEAGVDFNIGDINEISKHVA 305
                                           *******9976555667779***************************************************** PP

                             TIGR00110 287 llaklkPsgkkv.iedlhraGGvsavlkeldk.egllhkdaltvtGktlaetlekvkvlrvdqdvirsldnpv 357
                                           ++ak++Ps  +v +ed+++aGGvsav++e+++ ++ + +d ltvtG+tl e+++++k+l  d+++i+s++np+
  NCBI__GCF_000186245.1:WP_013553008.1 306 HIAKISPSLTTVhMEDINKAGGVSAVMHEMKRrRDSVLQDNLTVTGETLFERIADAKIL--DSEIIHSIENPY 376
                                           ********88888****************987678999999*****************9..************ PP

                             TIGR00110 358 kkegglavLkGnlaeeGavvkiagveedilkfeGpakvfeseeealeailggkvkeGdvvviryeGPkGgPGm 430
                                           ++ ggla+L+Gnlae+Gav+k+ag+      f+G+a  f+s++ea+e+i +gkvk G+vvviryeGPkGgPGm
  NCBI__GCF_000186245.1:WP_013553008.1 377 SEVGGLAILFGNLAEQGAVIKTAGIVG-ERSFTGKAICFDSQQEAIEGITSGKVKPGHVVVIRYEGPKGGPGM 448
                                           ************************987.589****************************************** PP

                             TIGR00110 431 remLaPtsalvglGLgkkvaLitDGrfsGgtrGlsiGhvsPeaaegGaialvedGDkikiDienrkldlevse 503
                                           +emLaPts ++g+GLg+kvaLitDGrfsG+trG siGhvsPeaaegG i+l++dGD+i++D+++  l++++s+
  NCBI__GCF_000186245.1:WP_013553008.1 449 QEMLAPTSLIMGMGLGDKVALITDGRFSGATRGASIGHVSPEAAEGGMIGLLKDGDEIHLDVDKYILEVRLSD 521
                                           ************************************************************************* PP

                             TIGR00110 504 eelaerrakakkkearevkgaLakyaklvssadkGavl 541
                                           ee+++rr++ ++k +   + +L++y+ lv++a++Gavl
  NCBI__GCF_000186245.1:WP_013553008.1 522 EEIEKRRKEFTPKVKPLSSRWLRQYRALVTNASRGAVL 559
                                           **************9999******************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (543 nodes)
Target sequences:                          1  (562 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 30.07
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis