Alignments for a candidate for carA in Desulforamulus ruminis DSM 2154

GapMind for Amino acid biosynthesis

Alignments for a candidate for carA in Desulforamulus ruminis DSM 2154

Align Carbamoyl-phosphate synthase pyrimidine-specific small chain; Carbamoyl-phosphate synthetase glutamine chain; EC 6.3.5.5 (characterized)
to candidate WP_013842338.1 DESRU_RS11820 glutamine-hydrolyzing carbamoyl-phosphate synthase small subunit

Query= SwissProt::P25993
         (364 letters)



>NCBI__GCF_000215085.1:WP_013842338.1
          Length = 371

 Score =  421 bits (1082), Expect = e-122
 Identities = 203/362 (56%), Positives = 269/362 (74%), Gaps = 4/362 (1%)

Query: 1   MKRRLVLENGAVFEGEAFGSLEHNMGEVVFNTGMTGYQEILSDPSYCGQIVTLTYPLIGN 60
           M+ RLVLE+G +F G AFG+     GEVVFNTGMTGYQE+L+DPSYCGQIV +TYPLIGN
Sbjct: 1   MQARLVLEDGTIFSGRAFGATGEQWGEVVFNTGMTGYQEVLTDPSYCGQIVVMTYPLIGN 60

Query: 61  YGINRDDFESITPFVKGLIIKELCELPSNWRSAYTLDEYLKMKNIPGLQGIDTRKLTRMI 120
           YGIN++DFE+ + FV+G ++KE C+ PSNWR +  +DE+L  + + G+ GIDTR LTR I
Sbjct: 61  YGINKEDFEAKSSFVRGFVVKEECDRPSNWRVSNKIDEFLAREGVIGIAGIDTRALTRKI 120

Query: 121 RTAGALKGTFASSDED-IEAVLKRLNETELPRNQ-VSQVSAKTAYPSPGRGKRIVLVDFG 178
           R+ G L+G  ++   D  E V K  N  ++   + V  V+ +  Y  PG G R+VL+DFG
Sbjct: 121 RSHGTLRGIISTEVSDPAELVAKAKNCPQISGQELVPTVATREIYTVPGNGHRVVLMDFG 180

Query: 179 MKHGILRELNKRKCDVIVVPYNITAEEVLQLKPDGIMLSNGPGDPKDVPEAIEMIKGVLG 238
            K  I+R LN R C+V+VVP +  AEE+L L+P GIMLSNGPGDP DVP+A+E ++ ++G
Sbjct: 181 AKANIVRCLNNRDCEVVVVPPDTAAEEILALQPKGIMLSNGPGDPTDVPKAVETVRRLIG 240

Query: 239 KVPLFGICLGHQLFALACGANTEKMKFGHRGSNHPVKELATGKVALTSQNHGYTVSSIS- 297
           ++P+FGICLGHQ+  LA G  T K+KFGHRG+NHPVK+L TG+V +TSQNHG+TV   S 
Sbjct: 241 QLPIFGICLGHQIIGLAMGGRTYKLKFGHRGANHPVKDLRTGRVYITSQNHGFTVDQDSL 300

Query: 298 KTELEVTHIAINDDTIEGLKHKTLPAFTVQYHPEASPGPEDANHLFDRFIEMI-ETTEKE 356
             E+EV+HI +ND T+EGL+HKTLP F+VQYHPEA+PGP D+ +LFD F+  I + + K 
Sbjct: 301 PPEMEVSHINLNDHTVEGLQHKTLPIFSVQYHPEAAPGPMDSEYLFDHFLNHIHDFSAKR 360

Query: 357 GE 358
           GE
Sbjct: 361 GE 362


Lambda     K      H
   0.317    0.136    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 442
Number of extensions: 18
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 364
Length of database: 371
Length adjustment: 30
Effective length of query: 334
Effective length of database: 341
Effective search space:   113894
Effective search space used:   113894
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 49 (23.5 bits)

Align candidate WP_013842338.1 DESRU_RS11820 (glutamine-hydrolyzing carbamoyl-phosphate synthase small subunit)
to HMM TIGR01368 (carA: carbamoyl-phosphate synthase, small subunit (EC 6.3.5.5))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01368.hmm
# target sequence database:        /tmp/gapView.1575814.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01368  [M=361]
Accession:   TIGR01368
Description: CPSaseIIsmall: carbamoyl-phosphate synthase, small subunit
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   2.1e-160  519.5   0.0   2.4e-160  519.3   0.0    1.0  1  NCBI__GCF_000215085.1:WP_013842338.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000215085.1:WP_013842338.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  519.3   0.0  2.4e-160  2.4e-160       1     360 [.       3     354 ..       3     355 .. 0.98

  Alignments for each domain:
  == domain 1  score: 519.3 bits;  conditional E-value: 2.4e-160
                             TIGR01368   1 atlvledGtvfegksfgaekevvGevvFnTsmtGYqEiltDpsYkgqivvltyplignygvneedaeskkikv 73 
                                           a lvledGt+f+g++fga++e+ GevvFnT+mtGYqE+ltDpsY gqivv+typlignyg+n+ed+e+k+  v
  NCBI__GCF_000215085.1:WP_013842338.1   3 ARLVLEDGTIFSGRAFGATGEQWGEVVFNTGMTGYQEVLTDPSYCGQIVVMTYPLIGNYGINKEDFEAKSSFV 75 
                                           579********************************************************************** PP

                             TIGR01368  74 kglvvkelskevsnyrakesLeeflkeegivaiegvDTRalvkklRekgsmkavisteksekeelvekakesp 146
                                           +g+vvke ++++sn+r +++++efl++eg+++i+g+DTRal++k+R++g+++++iste s+  elv+kak+ p
  NCBI__GCF_000215085.1:WP_013842338.1  76 RGFVVKEECDRPSNWRVSNKIDEFLAREGVIGIAGIDTRALTRKIRSHGTLRGIISTEVSDPAELVAKAKNCP 148
                                           ************************************************************************* PP

                             TIGR01368 147 kvkevnlvkevstkeayeleqkakkegkklrvvvidlGvKenilreLvkrgvevtvvpadtsaeeikklnpdg 219
                                           ++++++lv +v+t+e y++        + +rvv++d+G K+ni+r+L +r++ev+vvp dt aeei +l+p+g
  NCBI__GCF_000215085.1:WP_013842338.1 149 QISGQELVPTVATREIYTVPG------NGHRVVLMDFGAKANIVRCLNNRDCEVVVVPPDTAAEEILALQPKG 215
                                           *****************9994......4457****************************************** PP

                             TIGR01368 220 illsnGPGdPaaveeaietvkklleakiPifGIclGhqllalalgaktyklkfGhrGaNhpvkdlktgrveit 292
                                           i+lsnGPGdP++v +a+etv++l++ ++PifGIclGhq+++la+g++tyklkfGhrGaNhpvkdl+tgrv+it
  NCBI__GCF_000215085.1:WP_013842338.1 216 IMLSNGPGDPTDVPKAVETVRRLIG-QLPIFGICLGHQIIGLAMGGRTYKLKFGHRGANHPVKDLRTGRVYIT 287
                                           *************************.*********************************************** PP

                             TIGR01368 293 sqNHgyavdeeslkeeelevthvnlnDgtveglehkelpvfsvQyHPeaspGphdteylFdefvelik 360
                                           sqNHg++vd++sl+ e +ev h nlnD+tvegl+hk+lp+fsvQyHPea+pGp d+eylFd+f+++i+
  NCBI__GCF_000215085.1:WP_013842338.1 288 SQNHGFTVDQDSLPPE-MEVSHINLNDHTVEGLQHKTLPIFSVQYHPEAAPGPMDSEYLFDHFLNHIH 354
                                           ************9876.***********************************************9986 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (361 nodes)
Target sequences:                          1  (371 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 10.61
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the original publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis