GapMind for Amino acid biosynthesis

 

L-leucine biosynthesis in Pyrolobus fumarii 1A

Best path

ilvH, ilvI, ilvC, ilvD, leuA, leuC, leuD, leuB, ilvE

Rules

Overview: Leucine biosynthesis in GapMind is based on MetaCyc pathway L-leucine biosynthesis (link). This begins with the initial steps of valine biosynthesis (see link), up to 3-methyloxobutanoate.

9 steps (9 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
ilvH acetolactate/acetohydroxybutanoate synthase regulatory subunit PYRFU_RS08325
ilvI acetolactate/acetohydroxybutanoate synthase catalytic subunit PYRFU_RS08320
ilvC 2-hydroxy-3-ketol-acid reductoisomerase PYRFU_RS07845
ilvD dihydroxy-acid dehydratase PYRFU_RS08310
leuA 2-isopropylmalate synthase PYRFU_RS08315 PYRFU_RS09715
leuC 3-isopropylmalate dehydratase / citramalate isomerase, large subunit PYRFU_RS09265 PYRFU_RS00315
leuD 3-isopropylmalate dehydaratase / citramalate isomerase, small subunit PYRFU_RS03665 PYRFU_RS07965
leuB 3-methylmalate dehydrogenase / 3-isopropylmalate dehydrogenase PYRFU_RS09335 PYRFU_RS05270
ilvE leucine transaminase PYRFU_RS08035

Confidence: high confidence medium confidence low confidence
? – known gap: despite the lack of a good candidate for this step, this organism (or a related organism) performs the pathway

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory