Alignments for a candidate for gatA in Thioalkalivibrio thiocyanodenitrificans ARhD 1

GapMind for Amino acid biosynthesis

Alignments for a candidate for gatA in Thioalkalivibrio thiocyanodenitrificans ARhD 1

Align Glutamyl-tRNA(Gln) amidotransferase subunit A; Glu-ADT subunit A; EC 6.3.5.7 (uncharacterized)
to candidate WP_018232951.1 THITHI_RS0109995 amidase

Query= curated2:Q7UT33
         (499 letters)



>NCBI__GCF_000378965.1:WP_018232951.1
          Length = 521

 Score =  196 bits (498), Expect = 2e-54
 Identities = 160/503 (31%), Positives = 242/503 (48%), Gaps = 37/503 (7%)

Query: 7   EILKQLDSGEVTAVEVIAQSLAAIRASQPT--INAFTHVAEETAMQAAEAVDADRKAGKT 64
           E  + L SG  +  ++ A+ LA I A      +NA  H+    AM+ A+ +D   +    
Sbjct: 39  EAREGLRSGRFSCRQLTARHLARISAYDQAGGLNAIAHI-NPNAMRRADTLDRAWRDRGE 97

Query: 65  LGPLAGLPVAIKDVLCTSDMPTTCSSKMLEGFVPPYDATVVARLKSAGAIVVGKTNMDEF 124
           + PL  +PV +KD + T+DMPT   +  L+GFV   DA +V RL+ AGAIVV K+NM E+
Sbjct: 98  MAPLHCMPVILKDNMHTADMPTEAGAIALQGFVAAEDAFIVKRLREAGAIVVAKSNMGEW 157

Query: 125 AMGA-STETSAMGVTGNPWDTTKTPGGSSGGAAAAVAAGAVPLSLGTDTGGSIRQPAAFC 183
           A     T +S  G T N +D+ + P GSSGG A+A+AA    + LGTDTG SIR PAA  
Sbjct: 158 AFSPFHTISSTRGETRNAYDSERVPAGSSGGTASAIAAAFGIIGLGTDTGNSIRGPAAHL 217

Query: 184 GITGLKPTYGRVSRYGLVAFASSLDQAGPMGWSVDDVAIGLQAMAGYDPRDSTSVNAEV- 242
            + GL+PT G  SR+G+V    + D AGPM  +V+D AI    +AG+DP D  +   +  
Sbjct: 218 ALVGLRPTLGATSRHGIVPLLLNRDIAGPMTRTVEDAAITFSVIAGHDPADPLTERGKER 277

Query: 243 --PDFTPAMAAEDVRGMRIGVLREGLDQDGISPAVRDALATAESVFREQGAEIVEVELPH 300
              D+   +    + G R+GV+RE        P V   +  A    R+ GA +V+     
Sbjct: 278 LREDYRAYLDTGGLSGARLGVMRELYQTADADPEVMRIMEQALHDLRDAGAVLVD----- 332

Query: 301 SKYWVPTYYVIAPCEASSNLSRFDGAHYGYRVADAEIAAADSGPLEAMYSLSRAGGFGSE 360
             + V  + ++       +  RFD A++   + DA        P+  +  +  AG + + 
Sbjct: 333 -PFEVFDFEMLRNATGFCSRFRFDLANHLRDLGDA-------APVRTLDEIVSAGRYLAH 384

Query: 361 VKRRIM------VGTYALSEGYYDAYYNQALKVRRLIRNDYDAAF--QQVDLMLGPVTPS 412
            +R I       V  + +     D   +     RR + +    A   Q VD ++ P   +
Sbjct: 385 NERSIAWAMAVDVPPHEMQPPCVDVDGDPR---RRALLDAVLGAMDEQWVDAVIYPTWSN 441

Query: 413 PAFALNEKTDDPIAMYLCDLFTVGANLAGVPAISLPGGFDAVGLPVGVQLQAPVMEETRL 472
           P   + +  D P      +   V A   G PAI++P GF A GLP+G+Q  A   +E  L
Sbjct: 442 PPRRIGD-LDSPHG----NNSPVIAPHTGQPAITVPMGFTASGLPLGLQFLARPFDEHVL 496

Query: 473 LRAGNVFQMASDFHTRRPPTFTA 495
            R    ++  +  H R P  F A
Sbjct: 497 FRLAYAYEQHTQ-HRRPPARFGA 518


Lambda     K      H
   0.316    0.132    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 620
Number of extensions: 29
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 499
Length of database: 521
Length adjustment: 34
Effective length of query: 465
Effective length of database: 487
Effective search space:   226455
Effective search space used:   226455
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis