Alignments for a candidate for glnA in Thiomicrorhabdus arctica DSM 13458

GapMind for Amino acid biosynthesis

Alignments for a candidate for glnA in Thiomicrorhabdus arctica DSM 13458

Align glutamine synthetase (EC 6.3.1.2) (characterized)
to candidate WP_019556855.1 F612_RS0106010 type I glutamate--ammonia ligase

Query= BRENDA::P0A1P6
         (469 letters)



>NCBI__GCF_000381085.1:WP_019556855.1
          Length = 468

 Score =  613 bits (1581), Expect = e-180
 Identities = 282/466 (60%), Positives = 363/466 (77%)

Query: 3   AEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFFEEGKMFDGSSIGGWKGIN 62
           ++ +  ++ +++V + DLRFTDT GKEQHVTIPA  ++ +FF +G+ FDGSSI GWKGI+
Sbjct: 2   SQEIFDLIKDNDVTWADLRFTDTHGKEQHVTIPASAIDEDFFTDGETFDGSSIAGWKGIH 61

Query: 63  ESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYDRDPRSIAKRAEDYLRATGIA 122
            SDM+LMP     V+DPF  D T+IIRC +++P  ++ Y++DPR IAK+AE YL++TG+A
Sbjct: 62  NSDMILMPQTDGFVLDPFTNDPTIIIRCMVVDPSNMESYEKDPRGIAKKAEAYLKSTGVA 121

Query: 123 DTVLFGPEPEFFLFDDIRFGASISGSHVAIDDIEGAWNSSTKYEGGNKGHRPGVKGGYFP 182
           D+  FGPEPEFF+FDD+R+G  +SGS V ID  E +WNS   +  GN GHRPG+KGGYFP
Sbjct: 122 DSAFFGPEPEFFIFDDVRWGLDMSGSFVKIDADEASWNSEKVFANGNVGHRPGIKGGYFP 181

Query: 183 VPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAGQNEVATRFNTMTKKADEIQIYKYV 242
           VPPVD   ++R+++C + E MGL +EAHHHEVATAGQ E+A   NT+T KADE+QI KY 
Sbjct: 182 VPPVDQLHEVRADICAMSEAMGLKIEAHHHEVATAGQCELAIAGNTLTAKADEVQILKYA 241

Query: 243 VHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLAKNGTNLFSGDKYAGLSEQALYYIGGV 302
           VHN  H  GKTATFMPKP+ GDNG+GMH +MSL+K+G N+F+GD YAGLS++A++YIGG+
Sbjct: 242 VHNTVHALGKTATFMPKPIVGDNGNGMHINMSLSKDGKNIFAGDVYAGLSQEAIWYIGGL 301

Query: 303 IKHAKAINALANPTTNSYKRLVPGYEAPVMLAYSARNRSASIRIPVVASPKARRIEVRFP 362
           +KHA+A+NA  NP TNSYKRLVPG+EAP++LAYS  NRSASIRIP   S ++RR+E+RFP
Sbjct: 302 MKHARALNAFTNPGTNSYKRLVPGFEAPILLAYSGSNRSASIRIPYAPSERSRRVELRFP 361

Query: 363 DPAANPYLCFAALLMAGLDGIKNKIHPGEAMDKNLYDLPPEEAKEIPQVAGSLEEALNAL 422
           DP ANPYL FAA +MAGLDGI NKI PG   +K+LYDL PEE      V  SLEEAL+AL
Sbjct: 362 DPTANPYLAFAASMMAGLDGIINKIDPGAPSEKDLYDLTPEEEATYDTVCASLEEALDAL 421

Query: 423 DLDREFLKAGGVFTDEAIDAYIALRREEDDRVRMTPHPVEFELYYS 468
           + DR+FL A GVFTD  +D+YI L+ E   ++R T HP+EFELYYS
Sbjct: 422 NADRDFLTASGVFTDVMLDSYIELKMEHVTQLRATTHPIEFELYYS 467


Lambda     K      H
   0.318    0.136    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 733
Number of extensions: 29
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 469
Length of database: 468
Length adjustment: 33
Effective length of query: 436
Effective length of database: 435
Effective search space:   189660
Effective search space used:   189660
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

Align candidate WP_019556855.1 F612_RS0106010 (type I glutamate--ammonia ligase)
to HMM TIGR00653 (glnA: glutamine synthetase, type I (EC 6.3.1.2))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00653.hmm
# target sequence database:        /tmp/gapView.2719259.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00653  [M=462]
Accession:   TIGR00653
Description: GlnA: glutamine synthetase, type I
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
     6e-190  617.7   0.0   6.8e-190  617.6   0.0    1.0  1  NCBI__GCF_000381085.1:WP_019556855.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000381085.1:WP_019556855.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  617.6   0.0  6.8e-190  6.8e-190       2     462 .]       4     467 ..       3     467 .. 0.99

  Alignments for each domain:
  == domain 1  score: 617.6 bits;  conditional E-value: 6.8e-190
                             TIGR00653   2 evlkllkeenvkfvdlrfvDikGklkkveipvseleeealeegiaFDgssveGfksieesDlllkpdpetlvi 74 
                                           e+++l+k+++v   dlrf+D++Gk+++v+ip+s+++e+++++g +FDgss+ G+k+i++sD++l+p+++ +v 
  NCBI__GCF_000381085.1:WP_019556855.1   4 EIFDLIKDNDVTWADLRFTDTHGKEQHVTIPASAIDEDFFTDGETFDGSSIAGWKGIHNSDMILMPQTDGFVL 76 
                                           7999********************************************************************* PP

                             TIGR00653  75 vPfraekvlrvicdvyepvtkepyerdpRsiakraeeelk.tklgdevyfGpEaEFflfdkvefkeasnssfl 146
                                           +Pf+++++++++c v +p ++e+ye+dpR iak+ae +lk t+++d+++fGpE+EFf+fd+v+ +  ++ sf+
  NCBI__GCF_000381085.1:WP_019556855.1  77 DPFTNDPTIIIRCMVVDPSNMESYEKDPRGIAKKAEAYLKsTGVADSAFFGPEPEFFIFDDVRWGLDMSGSFV 149
                                           ************************************************************************* PP

                             TIGR00653 147 evdseegewn..reveegnkgykikkkggYfdvepvDkakdirrelvlaleelglevevsHHEvata.qaEid 216
                                           ++d+ e++wn  + + +gn g+++  kggYf+v+pvD+++++r +++ + e++gl++e++HHEvata q E++
  NCBI__GCF_000381085.1:WP_019556855.1 150 KIDADEASWNseKVFANGNVGHRPGIKGGYFPVPPVDQLHEVRADICAMSEAMGLKIEAHHHEVATAgQCELA 222
                                           **********98888999******************************************************* PP

                             TIGR00653 217 ikfdklvkaaDeivlyKyvvknvakkhGktatFmpKplfgdngsGmHvhlslwkdgenlfageegyagLseta 289
                                           i  ++l+ +aDe++++Ky+v+n+++  GktatFmpKp++gdng+GmH+++sl+kdg+n+fag+  yagLs++a
  NCBI__GCF_000381085.1:WP_019556855.1 223 IAGNTLTAKADEVQILKYAVHNTVHALGKTATFMPKPIVGDNGNGMHINMSLSKDGKNIFAGDV-YAGLSQEA 294
                                           ****************************************************************.******** PP

                             TIGR00653 290 lyyigGilkHakalaAltnptvnsYkRLvpGyEAPvylaysaknRsaliRiPasanpkakRiEvRspDpsanp 362
                                            +yigG++kHa+al+A+tnp +nsYkRLvpG+EAP+ lays +nRsa+iRiP++ +++++R+E+R+pDp+anp
  NCBI__GCF_000381085.1:WP_019556855.1 295 IWYIGGLMKHARALNAFTNPGTNSYKRLVPGFEAPILLAYSGSNRSASIRIPYAPSERSRRVELRFPDPTANP 367
                                           ************************************************************************* PP

                             TIGR00653 363 YLafaallmAgldGiknkidpgepldknlyelseeelkelGieqlpesLeealdelesdk..evlkevlgeel 433
                                           YLafaa +mAgldGi nkidpg+p +k+ly+l++ee +    +++ +sLeeald+l++d+  +++++v+++ +
  NCBI__GCF_000381085.1:WP_019556855.1 368 YLAFAASMMAGLDGIINKIDPGAPSEKDLYDLTPEEEAT--YDTVCASLEEALDALNADRdfLTASGVFTDVM 438
                                           ************************************999..9*****************988999******** PP

                             TIGR00653 434 ieafielkrkEveelrlkvhpvElekyld 462
                                           ++++ielk++ v +lr + hp+E+e y++
  NCBI__GCF_000381085.1:WP_019556855.1 439 LDSYIELKMEHVTQLRATTHPIEFELYYS 467
                                           ***************************95 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (462 nodes)
Target sequences:                          1  (468 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 32.15
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis