Alignments for a candidate for ilvI in Hippea alviniae EP5-r

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvI in Hippea alviniae EP5-r

Align acetohydroxyacid synthase subunit B (EC 2.2.1.6) (characterized)
to candidate WP_022669729.1 G415_RS0101065 biosynthetic-type acetolactate synthase large subunit

Query= metacyc::MONOMER-18810
         (585 letters)



>NCBI__GCF_000420385.1:WP_022669729.1
          Length = 565

 Score =  588 bits (1517), Expect = e-172
 Identities = 301/565 (53%), Positives = 395/565 (69%), Gaps = 4/565 (0%)

Query: 18  EMIGAEILVHALAEEGVEYVWGYPGGAVLYIYDELHKQTKFEHILVRHEQAAVHAADGYA 77
           ++ GA+I + AL +EGV++++G PGGA++ ++DE++KQ   + IL RHEQAAVH ADGYA
Sbjct: 2   KLTGAQIFIEALKKEGVKHLFGIPGGAIIDLHDEIYKQNDIKFILTRHEQAAVHMADGYA 61

Query: 78  RATGKVGVALVTSGPGVTNAVTGIATAYLDSIPMVVITGNVPTHAIGQDAFQECDTVGIT 137
           RATGK GV LVTSGPG TN VTG+ATAY+DSIP++V TG VPT  IG DAFQE D VG+T
Sbjct: 62  RATGKPGVVLVTSGPGATNTVTGLATAYMDSIPVIVFTGQVPTPLIGNDAFQEADIVGMT 121

Query: 138 RPIVKHNFLVKDVRDLAATIKKAFFIAATGRPGPVVVDIPKDVSRNACKYEYPKSIDMRS 197
           R   K+NFLVK V +LA TIKKAF+IA TGRPGPV++D PKDV     +++YP SI +R 
Sbjct: 122 RSCTKYNFLVKSVDELAYTIKKAFYIATTGRPGPVLIDFPKDVQVAQTEFKYPDSIHIRG 181

Query: 198 YNPVNKGHSGQIRKAVALLQGAERPYIYTGGGVVLANASDELRQLAALTGHPVTNTLMGL 257
           YNP   G+  Q++K V  +  A++P +Y GGG +++ A +E+ +LA     PV  TLMG+
Sbjct: 182 YNPTYHGNPKQMKKVVKAIASAKKPLLYVGGGAIISEAHNEIYRLAKKLQIPVFTTLMGI 241

Query: 258 GAFPGTSKQFVGMLGMHGTYEANMAMQNCDVLIAIGARFDDRVIGNPAHFTSQARKIIHI 317
           GA+P   +  +GM GMHGTY ANMA+Q CD+LI+IGARFDDRV G  + F   A KI+HI
Sbjct: 242 GAYPEDDELSLGMAGMHGTYRANMAIQYCDLLISIGARFDDRVTGKVSEFAPYA-KIVHI 300

Query: 318 DIDPSSISKRVKVDIPIVGNVKDVLQELIAQIKASDIKPKREALAKWWEQIEQWRSVDCL 377
           DIDP+SISK +KVD P+VG+ K VLQE++           +E    W EQIE+W++   L
Sbjct: 301 DIDPTSISKNIKVDYPLVGDAKLVLQEMLPMFDEYSSVNWKEIRKPWLEQIEKWKNEHRL 360

Query: 378 KYDRSSEIIKPQYVVEKIWELTK-GDAFICSDVGQHQMWAAQFYKFDEPRRWINSGGLGT 436
            Y+  ++ I PQYV+EK+ ELTK  D  I ++VGQHQMW AQFY F +PRR + SGGLGT
Sbjct: 361 AYNTETDEILPQYVIEKLGELTKDDDPIISTEVGQHQMWVAQFYTFKKPRRLLTSGGLGT 420

Query: 437 MGVGLPYAMGIKKAF-PEKEVVTITGEGSIQMCIQELSTCLQYDTPVKICSLNNGYLGMV 495
           MG G P  +G + A   +K V  I G+GS QM  QEL+T + Y+ PVK+  LNNGYLGMV
Sbjct: 421 MGYGFPAGIGAQFAVGDDKPVYVIAGDGSFQMNEQELATIVAYNKPVKVIILNNGYLGMV 480

Query: 496 RQWQEIEYDNRYSHSYMDALPDFVKLAEAYGHVGMRVEKTSDVEPALREAFRLKDRTVFL 555
           RQWQ++ Y  RY+++ ++  PDFVKLAE+YG    R+ K  +VE AL E  + K   + L
Sbjct: 481 RQWQQLFYGRRYANTNIEVQPDFVKLAESYGIKARRIRKKEEVEDALIEIRKHKGPYI-L 539

Query: 556 DFQTDPTENVWPMVQAGKGISEMLL 580
           D      ENV+PMV AG  ++ MLL
Sbjct: 540 DVIIAKEENVYPMVPAGAPLNNMLL 564


Lambda     K      H
   0.319    0.135    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 902
Number of extensions: 33
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 585
Length of database: 565
Length adjustment: 36
Effective length of query: 549
Effective length of database: 529
Effective search space:   290421
Effective search space used:   290421
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

Align candidate WP_022669729.1 G415_RS0101065 (biosynthetic-type acetolactate synthase large subunit)
to HMM TIGR00118 (ilvB: acetolactate synthase, large subunit, biosynthetic type (EC 2.2.1.6))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00118.hmm
# target sequence database:        /tmp/gapView.959643.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00118  [M=557]
Accession:   TIGR00118
Description: acolac_lg: acetolactate synthase, large subunit, biosynthetic type
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
     6e-260  849.4   0.5   6.8e-260  849.2   0.5    1.0  1  NCBI__GCF_000420385.1:WP_022669729.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000420385.1:WP_022669729.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  849.2   0.5  6.8e-260  6.8e-260       1     554 [.       3     563 ..       3     565 .] 0.98

  Alignments for each domain:
  == domain 1  score: 849.2 bits;  conditional E-value: 6.8e-260
                             TIGR00118   1 lkgaeilveslkkegvetvfGyPGGavlpiydaly.dselehilvrheqaaahaadGyarasGkvGvvlatsG 72 
                                           l+ga+i++e+lkkegv+++fG+PGGa+++++d++y ++++++il+rheqaa+h+adGyara+Gk+Gvvl+tsG
  NCBI__GCF_000420385.1:WP_022669729.1   3 LTGAQIFIEALKKEGVKHLFGIPGGAIIDLHDEIYkQNDIKFILTRHEQAAVHMADGYARATGKPGVVLVTSG 75 
                                           68*********************************999*********************************** PP

                             TIGR00118  73 PGatnlvtgiatayldsvPlvvltGqvatsliGsdafqeidilGitlpvtkhsflvkkaedlpeilkeafeia 145
                                           PGatn+vtg+atay+ds+P++v+tGqv+t liG+dafqe+di+G+t+++tk++flvk++++l+ ++k+af+ia
  NCBI__GCF_000420385.1:WP_022669729.1  76 PGATNTVTGLATAYMDSIPVIVFTGQVPTPLIGNDAFQEADIVGMTRSCTKYNFLVKSVDELAYTIKKAFYIA 148
                                           ************************************************************************* PP

                             TIGR00118 146 stGrPGPvlvdlPkdvteaeieleveekvelpgykptvkghklqikkaleliekakkPvllvGgGviiaease 218
                                           +tGrPGPvl+d+Pkdv+ a++e++++++++++gy+pt +g+++q+kk++++i++akkP+l+vGgG+ii+ea++
  NCBI__GCF_000420385.1:WP_022669729.1 149 TTGRPGPVLIDFPKDVQVAQTEFKYPDSIHIRGYNPTYHGNPKQMKKVVKAIASAKKPLLYVGGGAIISEAHN 221
                                           ************************************************************************* PP

                             TIGR00118 219 elkelaerlkipvtttllGlGafpedhplalgmlGmhGtkeanlavseadlliavGarfddrvtgnlakfape 291
                                           e+ +la++l+ipv ttl+G+Ga+ped++l+lgm+GmhGt+ an+a++ +dlli++Garfddrvtg++++fap 
  NCBI__GCF_000420385.1:WP_022669729.1 222 EIYRLAKKLQIPVFTTLMGIGAYPEDDELSLGMAGMHGTYRANMAIQYCDLLISIGARFDDRVTGKVSEFAPY 294
                                           ************************************************************************* PP

                             TIGR00118 292 akiihididPaeigknvkvdipivGdakkvleellkklkee...ekkeke..Wlekieewkkeyilkldeeee 359
                                           aki+hididP++i+kn+kvd p+vGdak vl+e+l    e+   + ke++  Wle+ie+wk+e+ l++++e +
  NCBI__GCF_000420385.1:WP_022669729.1 295 AKIVHIDIDPTSISKNIKVDYPLVGDAKLVLQEMLPMFDEYssvNWKEIRkpWLEQIEKWKNEHRLAYNTETD 367
                                           ***********************************99888766655555557********************* PP

                             TIGR00118 360 sikPqkvikelskllkd.eaivttdvGqhqmwaaqfyktkkprkfitsgGlGtmGfGlPaalGakvakpeet. 430
                                           +i Pq+vi++l +l+kd + i++t+vGqhqmw+aqfy++kkpr+ +tsgGlGtmG+G+Pa +Ga+ a+ +++ 
  NCBI__GCF_000420385.1:WP_022669729.1 368 EILPQYVIEKLGELTKDdDPIISTEVGQHQMWVAQFYTFKKPRRLLTSGGLGTMGYGFPAGIGAQFAVGDDKp 440
                                           ***************98689***********************************************987652 PP

                             TIGR00118 431 vvavtGdgsfqmnlqelstiveydipvkivilnnellGmvkqWqelfyeerysetklaselpdfvklaeayGv 503
                                           v  ++Gdgsfqmn qel+tiv y+ pvk++ilnn +lGmv+qWq+lfy +ry++t+++  +pdfvklae+yG+
  NCBI__GCF_000420385.1:WP_022669729.1 441 VYVIAGDGSFQMNEQELATIVAYNKPVKVIILNNGYLGMVRQWQQLFYGRRYANTNIEV-QPDFVKLAESYGI 512
                                           7789******************************************************6.************* PP

                             TIGR00118 504 kgiriekpeeleeklkealeskepvlldvevdkeeevlPmvapGagldelv 554
                                           k+ ri+k+ee+e++l e+ ++k+p++ldv ++kee+v+Pmv+ Ga l++++
  NCBI__GCF_000420385.1:WP_022669729.1 513 KARRIRKKEEVEDALIEIRKHKGPYILDVIIAKEENVYPMVPAGAPLNNML 563
                                           *************************************************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (557 nodes)
Target sequences:                          1  (565 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 26.01
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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Steps (tab-delimited)
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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis