Alignments for a candidate for cysK in Sedimenticola selenatireducens DSM 17993

GapMind for Amino acid biosynthesis

Alignments for a candidate for cysK in Sedimenticola selenatireducens DSM 17993

Align cysteine synthase (EC 2.5.1.47); L-3-cyanoalanine synthase (EC 4.4.1.9) (characterized)
to candidate WP_037374637.1 A3GO_RS0104205 cysteine synthase A

Query= BRENDA::Q84IF9
         (308 letters)



>NCBI__GCF_000428045.1:WP_037374637.1
          Length = 303

 Score =  305 bits (782), Expect = 7e-88
 Identities = 158/300 (52%), Positives = 208/300 (69%), Gaps = 2/300 (0%)

Query: 6   NSITELIGDTPAVKLNRIVDEDSADVYLKLEFMNPGSSVKDRIALAMIEAAEKAGKLKPG 65
           ++I + IG+TP ++LNR  D  +A++++KLE  NP  SVKDR AL MIE AE+ G+LKPG
Sbjct: 4   DNILQTIGNTPVIRLNRFPDTLAAELWVKLESFNPAGSVKDRPALNMIECAEQRGELKPG 63

Query: 66  DTIVEPTSGNTGIGLAMVAAAKGYKAVLVMPDTMSLERRNLLRAYGAELVLTPGAQGMRG 125
           DTI+EPTSGNTGIGLAM+AA KGY +V VM + MS ER+ +LRA+G +LVLTP  +G +G
Sbjct: 64  DTIIEPTSGNTGIGLAMIAAQKGYPSVFVMAEDMSEERKTILRAFGGKLVLTPAEKGTKG 123

Query: 126 PIAKAEELVREHGYFMPQQFKNEANPEIHRLTTGKEIVEQMGDQLDAFVAGVGTGGTTTG 185
            I +A++L  ++G+F   Q  N  NP  H+  T  EI    G +LDA V   GTGGT +G
Sbjct: 124 AIEEAKKLAAKNGWFFVGQHFNPDNPNAHK-GTADEIWADFGTRLDAIVCTTGTGGTISG 182

Query: 186 AGKVLREAYPNIKIYAVEPADSPVLSGGKPGPHKIQGIGAGFVPDILDTSIYDGVITVTT 245
            GK +R   P I+I A EPADSP+LS G    HKI G   GF+PDILDT IY+ +  +TT
Sbjct: 183 IGKYMRRHNPEIQIIATEPADSPILSQGIACKHKIMGTAPGFIPDILDTKIYNRIFQITT 242

Query: 246 EEAFAAARRAAREEGILGGISSGAAIHAALKVAKELG-KGKKVLAIIPSNGERYLSTPLY 304
           +EA+   RR A+ EGI  GISSGAA+   +K A+E   +GK +LA++P  GERYLST L+
Sbjct: 243 DEAYDTTRRLAQHEGIFAGISSGAAVAGMIKAAREEAFRGKIILAMLPDTGERYLSTDLW 302


Lambda     K      H
   0.314    0.134    0.374 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 276
Number of extensions: 9
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 308
Length of database: 303
Length adjustment: 27
Effective length of query: 281
Effective length of database: 276
Effective search space:    77556
Effective search space used:    77556
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 48 (23.1 bits)

Align candidate WP_037374637.1 A3GO_RS0104205 (cysteine synthase A)
to HMM TIGR01136 (cysteine synthase (EC 2.5.1.47))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01136.hmm
# target sequence database:        /tmp/gapView.2924656.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01136  [M=299]
Accession:   TIGR01136
Description: cysKM: cysteine synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   8.1e-124  398.8   0.0   9.1e-124  398.6   0.0    1.0  1  NCBI__GCF_000428045.1:WP_037374637.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000428045.1:WP_037374637.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  398.6   0.0  9.1e-124  9.1e-124       2     299 .]       7     302 ..       6     302 .. 0.99

  Alignments for each domain:
  == domain 1  score: 398.6 bits;  conditional E-value: 9.1e-124
                             TIGR01136   2 eeliGntPlvrln.lseelkaevlvKlEsrnPsgSvKdRialsmildAekrgllkkgktiieatSGNtGiaLA 73 
                                            ++iGntP++rln + + l+ae++vKlEs+nP+gSvKdR+al+mi+ Ae+rg+lk+g+tiie+tSGNtGi+LA
  NCBI__GCF_000428045.1:WP_037374637.1   7 LQTIGNTPVIRLNrFPDTLAAELWVKLESFNPAGSVKDRPALNMIECAEQRGELKPGDTIIEPTSGNTGIGLA 79 
                                           789**********999999****************************************************** PP

                             TIGR01136  74 mvaaakgyklilvmpetmslERrkllkayGaelvlteaeegmkgaiekakelaeeepekyvllkqfeNpaNpe 146
                                           m+aa+kgy  + vm e+ms+ER+++l+a+G +lvlt+ae+g+kgaie+ak+la++++  ++++ q+ Np+Np+
  NCBI__GCF_000428045.1:WP_037374637.1  80 MIAAQKGYPSVFVMAEDMSEERKTILRAFGGKLVLTPAEKGTKGAIEEAKKLAAKNG--WFFVGQHFNPDNPN 150
                                           *******************************************************76..99************ PP

                             TIGR01136 147 aHrkttgpEilkdtdgkidafvagvGtgGtitGvgrvlkekkpnvkivavePaespvlsegkpgphkiqgiga 219
                                           aH+  t+ Ei++d+  ++da+v ++GtgGti+G+g+++++++p+++i+a ePa+sp+ls+g + +hki g+ +
  NCBI__GCF_000428045.1:WP_037374637.1 151 AHK-GTADEIWADFGTRLDAIVCTTGTGGTISGIGKYMRRHNPEIQIIATEPADSPILSQGIACKHKIMGTAP 222
                                           **6.79******************************************************************* PP

                             TIGR01136 220 gfiPkildeelldevikvededaietarrlakeegilvGiSsGaavaaalkvakklekedkkivvilpdager 292
                                           gfiP+ild++++++++++++++a++t+rrla++egi++GiSsGaava ++k a+++++++k i+++lpd+ger
  NCBI__GCF_000428045.1:WP_037374637.1 223 GFIPDILDTKIYNRIFQITTDEAYDTTRRLAQHEGIFAGISSGAAVAGMIKAAREEAFRGKIILAMLPDTGER 295
                                           ************************************************************************* PP

                             TIGR01136 293 YLstelf 299
                                           YLst+l+
  NCBI__GCF_000428045.1:WP_037374637.1 296 YLSTDLW 302
                                           *****97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (299 nodes)
Target sequences:                          1  (303 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 12.57
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis