Alignments for a candidate for prpE in Azohydromonas australica DSM 1124

GapMind for Amino acid biosynthesis

Alignments for a candidate for prpE in Azohydromonas australica DSM 1124

Align Acetate--CoA ligase CCL3; HlCCL3; 2-methylbutanoate--CoA ligase CCL4; 2-methylpropanoate--CoA ligase CCL4; Butanoate--CoA ligase CCL3; Hexanoate--CoA ligase CCL3; Isovalerate--CoA ligase CCL3; Pentanoate--CoA ligase CCL3; Propionate--CoA ligase CCL3; EC 6.2.1.1; EC 6.2.1.-; EC 6.2.1.17 (characterized)
to candidate WP_028999882.1 H537_RS0124060 acyl-CoA synthetase

Query= SwissProt::M4IS88
         (568 letters)



>NCBI__GCF_000430725.1:WP_028999882.1
          Length = 547

 Score =  575 bits (1482), Expect = e-168
 Identities = 281/555 (50%), Positives = 373/555 (67%), Gaps = 21/555 (3%)

Query: 11  LPKNAANYTALTPLWFLERAATVHPTRTSVIHGSRHYTWLQTYHRCRQFASALNNHSIGL 70
           L KNAANY  L+PL +LERAA V+P R +++HG+   TW +T+ RCR+ ASAL    +G 
Sbjct: 11  LDKNAANYVPLSPLSYLERAAAVYPQRLALVHGALRQTWRETFERCRRLASALQRRGVGR 70

Query: 71  GSTVAVIAPNVPALYEAHFAVPMAGAVVNCVNIRLNASTIAFLLGHSSAAAVMVDQEFFS 130
           G TVA + PNVPA++EAHF VP AGAV+N +N RL+A +IAF+L H  A  ++ D+EF  
Sbjct: 71  GDTVAAMLPNVPAMFEAHFGVPAAGAVLNTLNTRLDAESIAFMLQHGEAKVLLTDREFAG 130

Query: 131 LAEEALKILAQESKSHYKPPLLVVIGDESCDPKTLEYALKTGAIEYEKFLEGGDPEFDWK 190
           +  +AL +L ++       PL+V + DE   P       + G + YE+ L GGDP   W 
Sbjct: 131 VVSKALALLGEQR------PLVVEVEDELAPPGP-----RLGEMSYEELLAGGDPAAPWT 179

Query: 191 PPEDEWQSISLGYTSGTTASPKGVVLSHRGAYLMSLSASVVWGINEGAIYLWTLPMFHCN 250
            P DEW +I+L YTSGTT +PKGVV  HRGAYL + S ++ W + +   YLWTLPMFHCN
Sbjct: 180 LPGDEWDAIALNYTSGTTGNPKGVVTHHRGAYLNAASNAIGWSLPQHPTYLWTLPMFHCN 239

Query: 251 GWCYTWGMAAFCGTNICLRQVTAKGVYSAIAKYGVTHFCAAPVVLNTIVNAPP--EEAII 308
           GWC+ W +A   GT++CLR+V    +Y  I ++ VTH C AP+V   +++AP    E I 
Sbjct: 240 GWCFPWTLALVAGTSVCLRKVDPALIYPLIREHHVTHMCGAPIVYGLLIHAPAALREGI- 298

Query: 309 PLPHLVHVMTAGAAPPPSVLFAMSEKGFKVAHTYGLSETYGPSTICAWKPEWDSLPPIKQ 368
              H +  + AGAAPP +V+      G  + H YGL+E YGP+ +CA + EW SLP  ++
Sbjct: 299 --GHEIKGLIAGAAPPAAVIEGCERIGIAITHVYGLTEVYGPAALCAQQEEWASLPLPER 356

Query: 369 ARLNARQGVRYIALEGLDVVDTKTMKPVPADGTTMGEIVMRGNAVMKGYLKNPKANEESF 428
           ARLNARQGV Y   + + V+DT TM+PVPADG T+GE+  RGNAVMKGYLKNP A +E+F
Sbjct: 357 ARLNARQGVPYPLQQAVTVLDTATMQPVPADGQTLGEVCFRGNAVMKGYLKNPAATQEAF 416

Query: 429 ADGWFHSGDLAVKHPDGYIEIKDRSKDIIISGGENISSLEVENTLYLHPAVLEVSVVARP 488
             GWF +GDLAV HPDGY++I DRSKD+IISGGENISSLEVE+ L+ HP V+  +VVA+P
Sbjct: 417 EGGWFRTGDLAVLHPDGYVKITDRSKDVIISGGENISSLEVEDVLHRHPDVMLAAVVAQP 476

Query: 489 DERWGESPCAFVTLKPNIDKSNEQVLAEDIIKFCKSKMPAYWVPKSVVFGPLPKTATGKI 548
           D +WGE PCAFV +     K    V  +++I+FC+  +  +  PK +VFG LPKT+TGKI
Sbjct: 477 DPKWGEVPCAFVEV-----KLGSTVTEDELIEFCRGHLARFKAPKRIVFGELPKTSTGKI 531

Query: 549 QKHVLRAKAKEMGAL 563
           QK VLR   K   A+
Sbjct: 532 QKFVLRQALKSASAI 546


Lambda     K      H
   0.318    0.134    0.415 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 891
Number of extensions: 33
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 568
Length of database: 547
Length adjustment: 36
Effective length of query: 532
Effective length of database: 511
Effective search space:   271852
Effective search space used:   271852
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the original publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis