Alignments for a candidate for prpE in Azohydromonas australica DSM 1124

GapMind for Amino acid biosynthesis

Alignments for a candidate for prpE in Azohydromonas australica DSM 1124

Align 4-hydroxybutyrate-CoA ligase (AMP-forming) (EC 6.2.1.40) (characterized)
to candidate WP_043458035.1 H537_RS0102685 AMP-binding protein

Query= BRENDA::A4YDT1
         (564 letters)



>NCBI__GCF_000430725.1:WP_043458035.1
          Length = 574

 Score =  405 bits (1040), Expect = e-117
 Identities = 229/527 (43%), Positives = 314/527 (59%), Gaps = 16/527 (3%)

Query: 35  LRRFNWVRDVFEDIHVKERGSKTALIWRDINTGEEAKLSYHELSLMSNRVLSTLRKHGLK 94
           L +FNW  D F+ +    R +    +W     G E K S+ ++S  S +V + LR+HG+K
Sbjct: 39  LEQFNWALDHFDAM---ARDNDAPALWIVDEHGGELKRSFRQMSERSAQVANFLRRHGVK 95

Query: 95  KGDVVYLMTKVHPMHWAVFLAVIKGGFVMVPSATNLTVAEMKYRFSDLKPSAIISDSLRA 154
           +GD V LM       W + L   K G V++P+   LT  +++ R        +++ S  +
Sbjct: 96  RGDRVLLMLGNEVALWELMLGAFKLGAVVIPATALLTPDDLRDRLERGNVRHVVAGSALS 155

Query: 155 SVMEEALGSLKVEKFLIDGKRETWNSL---EDESSNAEPED-TRGEDVIINYFTSGTTGM 210
           +  E         +  +   +  W      ++ES++ EP+  T   D ++ YFTSGTT  
Sbjct: 156 AKFEGLADGCT--RLCVGAAQPGWLDFALSQEESAHFEPDGPTAANDPLLLYFTSGTTSK 213

Query: 211 PKRVIHTAVSYPVGSITTASIVGVRESDLHLNLSATGWAKFAWSSFFSPLLVGATVVGIN 270
           PK V+HT  SYPVG ++T   +G++  D+HLN+S+ GWAK AWS FF+P   GA V  I+
Sbjct: 214 PKLVLHTHQSYPVGHLSTMYWIGLQPGDVHLNISSPGWAKHAWSCFFAPWNAGACVF-IH 272

Query: 271 YEGKLDTRRYLGEVENLGVTSFCAPPTAWRQFITLDLDQFRFE-RLRSVVSAGEPLNPEV 329
              +      LG +E  GVT+ CAPPT WR  I  D+  +R   +LR V+ AGEPLNPE+
Sbjct: 273 NVARFSASALLGVLERHGVTTLCAPPTVWRMVIQEDMAAWRGRLKLREVIGAGEPLNPEI 332

Query: 330 IKIWKDKFNLTIRDFYGQTETTAMVGNFPFLKVKPGSMGKPHPLYDIRLLDDEGKEITKP 389
           I+  ++ + L++RD YGQTETTA VGN P   +KPGSMG+P P Y + LLD +G E T+ 
Sbjct: 333 IEQVREAWGLSLRDGYGQTETTAQVGNAPGQPLKPGSMGRPLPGYQVALLDMDGNESTE- 391

Query: 390 YEVGHITVKLNPRPIGLFLGYSDE-KKNMESFREGYYYTGDKAYFDEEGYFYFVGRGDDV 448
              G + +KL PRP+GL  GY D  +K  E  R+GYY TGD A  D EGY  FVGR DDV
Sbjct: 392 ---GEVCLKLTPRPLGLMAGYEDSAEKTAEVMRDGYYRTGDVAMRDAEGYLTFVGRADDV 448

Query: 449 IKTSDYRVGPFEVESALLEHPAVAEAAVVGVPDTVRWQLVKAYIVLKKGYMPSKELAEEI 508
            K SDYR+ PFE+ES L+EHPAV E AVV  PD VR  + KA+I L +G      LA ++
Sbjct: 449 FKASDYRISPFELESVLMEHPAVTEVAVVPSPDPVRLAVPKAFITLAQGREADAALALDV 508

Query: 509 REKMKTLLSPYKVPRIIEFVDELPKTISGKIRRVELRKREEEKRKKG 555
               +  L+PYK  R IEF+ ELPKTISGK+RRVELR+ E E+R +G
Sbjct: 509 LGWARRQLAPYKRVRRIEFLTELPKTISGKLRRVELRRLEAERRNQG 555


Lambda     K      H
   0.318    0.137    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 729
Number of extensions: 32
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 564
Length of database: 574
Length adjustment: 36
Effective length of query: 528
Effective length of database: 538
Effective search space:   284064
Effective search space used:   284064
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the original publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis