Alignments for a candidate for aspS2 in Dechloromonas agitata is5

GapMind for Amino acid biosynthesis

Alignments for a candidate for aspS2 in Dechloromonas agitata is5

Align Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 (characterized)
to candidate WP_027457591.1 K420_RS0108005 aspartate--tRNA ligase

Query= SwissProt::Q51422
         (591 letters)



>NCBI__GCF_000519045.1:WP_027457591.1
          Length = 599

 Score =  793 bits (2047), Expect = 0.0
 Identities = 393/594 (66%), Positives = 473/594 (79%), Gaps = 5/594 (0%)

Query: 2   MRSHYCGQLNESLDGQEVTLCGWVHRRRDHGGVIFLDVRDREGLAQVVFDPDRAETFAKA 61
           MR+HYCGQLN SLDGQ VTLCGW HRRRDHGGVIF+D+RDREGLAQVV DPDR + F  A
Sbjct: 1   MRTHYCGQLNASLDGQIVTLCGWAHRRRDHGGVIFIDLRDREGLAQVVCDPDRVDAFKIA 60

Query: 62  DRVRSEFVVKITGKVRLRPEGARNPNMASGSIEVLGYELEVLNQAETPPFPLDEYSDVGE 121
           + VR+EF +KITGKVR RP G  N N+ASG IEVL +E+EVLN + TPPF LDE  ++ E
Sbjct: 61  ESVRNEFCLKITGKVRPRPAGTTNANLASGEIEVLCHEIEVLNPSVTPPFQLDE-ENLSE 119

Query: 122 ETRLRYRFIDLRRPEMAAKLKLRARITSSIRRYLDDNGFLDVETPILGRPTPEGARDYLV 181
             RL +R IDLRRP+M   L LR +   + RR+LDDNGF+DVETP+L + TPEGARDYLV
Sbjct: 120 NVRLTHRVIDLRRPQMQNNLMLRYKTARAFRRFLDDNGFIDVETPMLTKSTPEGARDYLV 179

Query: 182 PSRTYPGHFFALPQSPQLFKQLLMVAGFDRYYQIAKCFRDEDLRADRQPEFTQIDIETSF 241
           PSR +PG FFALPQSPQLFKQLLMVAGFDRYYQI KCFRDEDLRADRQPEFTQ+DIETSF
Sbjct: 180 PSRVHPGQFFALPQSPQLFKQLLMVAGFDRYYQITKCFRDEDLRADRQPEFTQVDIETSF 239

Query: 242 LDESDIIGITEKMVRQLFKEVLDVEFDEFPHMPFEEAMRRYGSDKPDLRIPLELVDVADQ 301
           + E +I  + EK++R++FKE +DV+  EFP M + EAMRR+GSDKPDLR+ LELVDVAD 
Sbjct: 240 MSEDEITALMEKLIRRVFKEAIDVDLPEFPRMTYAEAMRRFGSDKPDLRVTLELVDVADA 299

Query: 302 LKEVEFKVFSGPANDPKGRVAALRVPGAASMPRSQIDDYTKFVGIYGAKGLAYIKVNERA 361
            K+V FKVF+  AN   GR+AA+R+PG A++ R +ID+YTKFVGIYGAKGLAYIKVN+  
Sbjct: 300 FKDVAFKVFASVANSEGGRIAAMRIPGGAALTRGEIDEYTKFVGIYGAKGLAYIKVNDVT 359

Query: 362 KGVE-GLQSPIVKFIPEANLNVILDRVGAVDGDIVFFGADKAKIVCDALGALRIKVGHDL 420
           +  E GLQSPIVK + EA+L  +L+R GA  GD++FFGADKAKIV DALGALRIK+GH+ 
Sbjct: 360 QVNETGLQSPIVKNLSEASLRTVLERTGAQSGDLIFFGADKAKIVSDALGALRIKIGHEK 419

Query: 421 KLLT-REWAPMWVVDFPMFEENDDGS-LSALHHPFTSPKCTPAE-LEANPGAALSRAYDM 477
             +T  +WAP+WV+DFPMFE +D+    +A HHPFTSPK    E L  +PG  L++AYD+
Sbjct: 420 GFVTGAKWAPLWVIDFPMFEYDDESKRWTACHHPFTSPKDEHLEFLATDPGKCLAKAYDL 479

Query: 478 VLNGTELGGGSIRIHDKSMQQAVFRVLGIDEAEQEEKFGFLLDALKYGAPPHGGLAFGLD 537
            LNG ELGGGS+RIH   +Q+ VF  L I   EQ+ KFGFLLDALKYGAPPHGGLAFGLD
Sbjct: 480 ALNGWELGGGSVRIHRADVQEKVFAALNIGPEEQQAKFGFLLDALKYGAPPHGGLAFGLD 539

Query: 538 RLVMLMTGASSIREVIAFPKTQSAGDVMTQAPGSVDGKALRELHIRLREQPKAE 591
           R+V +MTGA SIR+VIAFPKTQ A  ++T AP +VD K LRELH+RLR++ + +
Sbjct: 540 RIVTMMTGAESIRDVIAFPKTQRAQCLLTDAPSAVDEKQLRELHVRLRQKVETQ 593


Lambda     K      H
   0.321    0.140    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1105
Number of extensions: 47
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 591
Length of database: 599
Length adjustment: 37
Effective length of query: 554
Effective length of database: 562
Effective search space:   311348
Effective search space used:   311348
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis