Alignments for a candidate for aroC in Thiothrix lacustris DSM 21227

GapMind for Amino acid biosynthesis

Alignments for a candidate for aroC in Thiothrix lacustris DSM 21227

Align Chorismate synthase; CS; 5-enolpyruvylshikimate-3-phosphate phospholyase; EPSP phospholyase; EC 4.2.3.5 (characterized)
to candidate WP_028490321.1 Q394_RS0117130 chorismate synthase

Query= SwissProt::P12008
         (361 letters)



>NCBI__GCF_000621325.1:WP_028490321.1
          Length = 365

 Score =  530 bits (1366), Expect = e-155
 Identities = 255/359 (71%), Positives = 301/359 (83%)

Query: 1   MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHDLDRRRPGTSRYTTQRR 60
           M+GN+ G+LF VT+FGESHG A+GCIVDG PPG+ LTEAD+Q DLDRR+PG SR+TTQRR
Sbjct: 1   MSGNSFGKLFTVTSFGESHGPAIGCIVDGCPPGLALTEADIQIDLDRRKPGQSRHTTQRR 60

Query: 61  EPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVFRPGHADYTYEQKYGLRDY 120
           E D+V+ILSGVFEG TTGT+I L+I NTDQRS+DY  I D FRPGHADYTY +KYG RDY
Sbjct: 61  EADEVQILSGVFEGKTTGTTIALIIHNTDQRSKDYGDIMDRFRPGHADYTYYKKYGFRDY 120

Query: 121 RGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQMGDIPLDIKDWSQVEQNPFFC 180
           RGGGRSSARETAMRVAAG+IAKK+L E++G+ IRG L+Q+G I  +  DW ++  NPFFC
Sbjct: 121 RGGGRSSARETAMRVAAGSIAKKWLFERYGVVIRGYLSQLGPIVAETLDWDEIANNPFFC 180

Query: 181 PDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGLGEPVFDRLDADIAHALMSINAV 240
           PD  K+  +++ M AL+KEG+S+GAK+T VAS VP G GEP+FDRLDADIAHA+MSINA 
Sbjct: 181 PDASKVQPMEDYMDALRKEGNSVGAKITTVASHVPPGWGEPIFDRLDADIAHAMMSINAA 240

Query: 241 KGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGILGGISSGQQIIAHMALKPTSSITV 300
           KGVEIG GFD VA +G+++RDEIT  GF SNHAGGILGGISSGQ++I H A KPTSS+ +
Sbjct: 241 KGVEIGAGFDCVAQKGTEHRDEITMQGFLSNHAGGILGGISSGQEVIVHTAFKPTSSMRL 300

Query: 301 PGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEAMLAIVLMDHLLRQRAQNADVKTDIP 359
           PGRT+N  GE VE+ITKGRHDPCVGIRA PI EAMLAI LMDH LR RAQNADV TD+P
Sbjct: 301 PGRTVNLDGEAVEVITKGRHDPCVGIRATPICEAMLAITLMDHALRHRAQNADVTTDLP 359


Lambda     K      H
   0.319    0.138    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 484
Number of extensions: 14
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 361
Length of database: 365
Length adjustment: 29
Effective length of query: 332
Effective length of database: 336
Effective search space:   111552
Effective search space used:   111552
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 49 (23.5 bits)

Align candidate WP_028490321.1 Q394_RS0117130 (chorismate synthase)
to HMM TIGR00033 (aroC: chorismate synthase (EC 4.2.3.5))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00033.hmm
# target sequence database:        /tmp/gapView.2048395.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00033  [M=351]
Accession:   TIGR00033
Description: aroC: chorismate synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   2.4e-142  460.0   0.0   2.7e-142  459.8   0.0    1.0  1  NCBI__GCF_000621325.1:WP_028490321.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000621325.1:WP_028490321.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  459.8   0.0  2.7e-142  2.7e-142       1     350 [.      10     350 ..      10     351 .. 0.98

  Alignments for each domain:
  == domain 1  score: 459.8 bits;  conditional E-value: 2.7e-142
                             TIGR00033   1 lrlttfGeSHgkalgaiidGlPaglelteediqkelkrRrpgqsrltrmrkEeDeveilsGvfeGkTtGaPia 73 
                                           +++t fGeSHg+a+g+i+dG+P+gl lte+diq +l+rR+pgqsr+t++r+E+Dev+ilsGvfeGkTtG+ ia
  NCBI__GCF_000621325.1:WP_028490321.1  10 FTVTSFGESHGPAIGCIVDGCPPGLALTEADIQIDLDRRKPGQSRHTTQRREADEVQILSGVFEGKTTGTTIA 82 
                                           789********************************************************************** PP

                             TIGR00033  74 llikNkdvrskdyedikelpRPgHadytylkKYgikdregggrsSaReTaarvaaGavakklLketagieiva 146
                                           l+i+N+d+rskdy di +++RPgHadyty kKYg++d++gggrsSaReTa+rvaaG++akk+L e +g+ i +
  NCBI__GCF_000621325.1:WP_028490321.1  83 LIIHNTDQRSKDYGDIMDRFRPGHADYTYYKKYGFRDYRGGGRSSARETAMRVAAGSIAKKWLFERYGVVIRG 155
                                           ************************************************************************* PP

                             TIGR00033 147 yvvklgeveleeesakeiskerldkspvrcpdaeaekemeeeidkakkdgdsvGgvvevvvsnvpvglGeplf 219
                                           y+++lg + +e+        ++++++p++cpda++ + me+++d ++k+g+svG+++++v+s vp g+Gep+f
  NCBI__GCF_000621325.1:WP_028490321.1 156 YLSQLGPIVAETLDW-----DEIANNPFFCPDASKVQPMEDYMDALRKEGNSVGAKITTVASHVPPGWGEPIF 223
                                           ********9996444.....46999************************************************ PP

                             TIGR00033 220 dkldaelasallsinAvKgveiGdGFeaasvrGseanDelvleddkirrktnnsGGieGGitnGedirvriav 292
                                           d+lda +a+a++sinA+KgveiG+GF+ + ++G e+ De++++     + +n+ GGi+GGi+ G+++ v+ a+
  NCBI__GCF_000621325.1:WP_028490321.1 224 DRLDADIAHAMMSINAAKGVEIGAGFDCVAQKGTEHRDEITMQ----GFLSNHAGGILGGISSGQEVIVHTAF 292
                                           **************************************99885....79************************ PP

                             TIGR00033 293 KpiptikkplktvdletkekakatkgRhDpcvvpravpvvEamvalvladallekras 350
                                           Kp+++++ p +tv+l+++    +tkgRhDpcv +ra+p++Eam+a++l+d++l++ra+
  NCBI__GCF_000621325.1:WP_028490321.1 293 KPTSSMRLPGRTVNLDGEAVEVITKGRHDPCVGIRATPICEAMLAITLMDHALRHRAQ 350
                                           ***************99999999********************************987 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (351 nodes)
Target sequences:                          1  (365 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 22.02
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis