Alignments for a candidate for metX in Beijerinckia mobilis UQM 1969

GapMind for Amino acid biosynthesis

Alignments for a candidate for metX in Beijerinckia mobilis UQM 1969

Align Homoserine O-acetyltransferase; HAT; Homoserine transacetylase; HTA; EC 2.3.1.31 (characterized)
to candidate WP_280949214.1 DL88_RS17420 homoserine O-acetyltransferase

Query= SwissProt::Q9AAS1
         (382 letters)



>NCBI__GCF_000745425.1:WP_280949214.1
          Length = 411

 Score =  504 bits (1297), Expect = e-147
 Identities = 240/367 (65%), Positives = 295/367 (80%), Gaps = 2/367 (0%)

Query: 16  RFPANEPLRLDSGGVIEGLEIAYQTYGQLNADKSNAVLICHALTGDQHVASPHPTTGKPG 75
           RF  ++PL +D+G  +  L IAYQTYG LN DKSNA+L+CHALTGDQHVAS HP TGKPG
Sbjct: 22  RFGPDQPLAMDAGVSLAPLTIAYQTYGTLNEDKSNAILVCHALTGDQHVASDHPITGKPG 81

Query: 76  WWQRLVGPGKPLDPARHFIICSNVIGGCMGSTGPASINPATGKTYGLSFPVITIADMVRA 135
           WW  +VGPG+P+D  R+F+I SNV+GGCMG+TGPAS+NPATG+ +GL  P++TI DMV+A
Sbjct: 82  WWWIMVGPGRPIDTNRYFVISSNVVGGCMGTTGPASLNPATGRAWGLDLPIVTIRDMVKA 141

Query: 136 QAMLVSALGVETLFAVVGGSMGGMQVQQWAVDYPERMFSAVVLASASRHSAQNIAFHEVG 195
           QAML+  LG++TLF V GGSMGGMQV QWA  +P R+F+A+ +A+A++HS+QNIAFHEVG
Sbjct: 142 QAMLIDHLGIKTLFCVAGGSMGGMQVLQWAASFPGRVFAAMPIATAAKHSSQNIAFHEVG 201

Query: 196 RQAIMADPDWRGGAYAEHGVRPEKGLAVARMAAHITYLSEPALQRKFGRELQ-RDGLSWG 254
           RQ+IMADP+W  G Y + G  P KGLAVARMAAHITYLS+ ALQ KFGR+LQ R   ++ 
Sbjct: 202 RQSIMADPNWLAGRYLDQGTFPNKGLAVARMAAHITYLSDEALQSKFGRKLQGRSAPTFS 261

Query: 255 FDADFQVESYLRHQGSSFVDRFDANSYLYITRAMDYFDIAASHGGVLAKAFTRARNVRFC 314
           FDADFQVE+YLRHQG+SFVDRFDANSYLY+TRA DYFD+AA +GG LA AF +    RFC
Sbjct: 262 FDADFQVENYLRHQGASFVDRFDANSYLYVTRACDYFDLAADYGGSLAMAF-KGTKTRFC 320

Query: 315 VLSFSSDWLYPTAENRHLVRALTAAGARAAFAEIESDKGHDAFLLDEPVMDAALEGFLAS 374
           V+SF SDWLY TA++R +V AL A GA  +F +IE+D+GHDAFLL+EP   A   GFL +
Sbjct: 321 VVSFQSDWLYTTADSRAIVHALNAGGASVSFVDIETDRGHDAFLLNEPEFIATTRGFLDA 380

Query: 375 AERDRGL 381
           A R RGL
Sbjct: 381 AARARGL 387


Lambda     K      H
   0.321    0.135    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 505
Number of extensions: 16
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 382
Length of database: 411
Length adjustment: 31
Effective length of query: 351
Effective length of database: 380
Effective search space:   133380
Effective search space used:   133380
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 50 (23.9 bits)

Align candidate WP_280949214.1 DL88_RS17420 (homoserine O-acetyltransferase)
to HMM TIGR01392 (metX: homoserine O-acetyltransferase (EC 2.3.1.31))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01392.hmm
# target sequence database:        /tmp/gapView.249479.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01392  [M=351]
Accession:   TIGR01392
Description: homoserO_Ac_trn: homoserine O-acetyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   9.6e-142  458.1   0.0   1.1e-141  457.9   0.0    1.0  1  NCBI__GCF_000745425.1:WP_280949214.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000745425.1:WP_280949214.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  457.9   0.0  1.1e-141  1.1e-141       3     350 ..      27     378 ..      25     379 .. 0.97

  Alignments for each domain:
  == domain 1  score: 457.9 bits;  conditional E-value: 1.1e-141
                             TIGR01392   3 eeltlesGevlsevevayktyGtlnaerdNavlvcHaltgsahvagkadeedk.GWWdellGpgraldtsryf 74 
                                           ++l +++G  l  +++ay+tyGtln++++Na+lvcHaltg++hva+ +  + k GWW  ++Gpgr++dt+ryf
  NCBI__GCF_000745425.1:WP_280949214.1  27 QPLAMDAGVSLAPLTIAYQTYGTLNEDKSNAILVCHALTGDQHVASDHPITGKpGWWWIMVGPGRPIDTNRYF 99 
                                           7899*****************************************888765556******************* PP

                             TIGR01392  75 vvclNvlGsckGstgPlsinpetgkpygaefPlvtirDlvkaqkalldsLgveklaavvGgSlGGmqalewal 147
                                           v+++Nv+G+c G+tgP+s+np+tg+ +g ++P vtirD+vkaq++l+d+Lg+++l++v GgS+GGmq+l+wa+
  NCBI__GCF_000745425.1:WP_280949214.1 100 VISSNVVGGCMGTTGPASLNPATGRAWGLDLPIVTIRDMVKAQAMLIDHLGIKTLFCVAGGSMGGMQVLQWAA 172
                                           ************************************************************************* PP

                             TIGR01392 148 sypervkkivvlatsarasaqaiafnevqrqailsDpeyndGeyaeee.qPekGLalARmlalltYrseesle 219
                                           s p rv +++++at+a++s+q+iaf+ev rq+i++Dp++  G+y +++  P+kGLa+ARm a++tY+s+e+l+
  NCBI__GCF_000745425.1:WP_280949214.1 173 SFPGRVFAAMPIATAAKHSSQNIAFHEVGRQSIMADPNWLAGRYLDQGtFPNKGLAVARMAAHITYLSDEALQ 245
                                           **********************************************9999*********************** PP

                             TIGR01392 220 erfgreakseeslassleeefsvesylryqgkkfverFdAnsYllltkaldthdlargrrdslkealkkikap 292
                                           ++fgr+ + ++++++s++++f+ve ylr+qg +fv+rFdAnsYl++t+a d++dla+++ +sl+ a+k +k++
  NCBI__GCF_000745425.1:WP_280949214.1 246 SKFGRKLQGRSAPTFSFDADFQVENYLRHQGASFVDRFDANSYLYVTRACDYFDLAADYGGSLAMAFKGTKTR 318
                                           ************************************************************************* PP

                             TIGR01392 293 vlvvgiesDllftleeqeelakalkaakle..yaeieseeGHDaFllekekveelirefl 350
                                           ++vv+++sD+l+t+++++ +++al+a +++  ++ ie+++GHDaFll++ ++ ++ r fl
  NCBI__GCF_000745425.1:WP_280949214.1 319 FCVVSFQSDWLYTTADSRAIVHALNAGGASvsFVDIETDRGHDAFLLNEPEFIATTRGFL 378
                                           *************************9998888*****************99988877776 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (351 nodes)
Target sequences:                          1  (411 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 14.84
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis