Alignments for a candidate for metX in Xenophilus azovorans DSM 13620

GapMind for Amino acid biosynthesis

Alignments for a candidate for metX in Xenophilus azovorans DSM 13620

Align Homoserine O-acetyltransferase; HAT; Homoserine transacetylase; HTA; EC 2.3.1.31 (characterized)
to candidate WP_038202407.1 Q392_RS05130 homoserine O-acetyltransferase

Query= SwissProt::Q9AAS1
         (382 letters)



>NCBI__GCF_000745855.1:WP_038202407.1
          Length = 374

 Score =  362 bits (929), Expect = e-105
 Identities = 179/344 (52%), Positives = 238/344 (69%), Gaps = 7/344 (2%)

Query: 22  PLRLDSGGVIEGLEIAYQTYGQLNADKSNAVLICHALTGDQHVASPHPTTGKP---GWWQ 78
           PL L SG  +    +AY+TYG LNA++SNAVL+CHAL    HVA  +   G+P   GWW 
Sbjct: 15  PLALRSGASLGDYTLAYETYGTLNAERSNAVLVCHALNASHHVAGRY--AGQPKSEGWWD 72

Query: 79  RLVGPGKPLDPARHFIICSNVIGGCMGSTGPASINPATGKTYGLSFPVITIADMVRAQAM 138
            ++GPGK +D  R F+I  N +G C GSTGP  +NPATG+ YG  FPV+T+ D V AQA 
Sbjct: 73  NMIGPGKAVDTDRFFVIGINNLGSCFGSTGPMHVNPATGRVYGADFPVVTVEDWVDAQAR 132

Query: 139 LVSALGVETLFAVVGGSMGGMQVQQWAVDYPERMFSAVVLASASRHSAQNIAFHEVGRQA 198
           L+ ALG++TL AV+GGS+GGMQ   W + YPER+  A V+ASA   +A+NIAF+EV R+A
Sbjct: 133 LLDALGIDTLAAVMGGSLGGMQALSWTLQYPERVRHAAVIASAPNLNAENIAFNEVARRA 192

Query: 199 IMADPDWRGGAYAEHGVRPEKGLAVARMAAHITYLSEPALQRKFGRELQRDGLSWGF-DA 257
           I+ DPD+ GG + EHGV P++GL +ARM  HITYLS+  +  KFGREL+   + +   D 
Sbjct: 193 IVTDPDFHGGHFYEHGVVPKRGLRIARMIGHITYLSDDVMNEKFGRELREGVVRYTTQDV 252

Query: 258 DFQVESYLRHQGSSFVDRFDANSYLYITRAMDYFDIAASHGGVLAKAFTRARNVRFCVLS 317
           +FQ+ESYLR+QG  F + FDAN+YL ITRA+DYFD A  HGG LA A  RA   +F ++S
Sbjct: 253 EFQIESYLRYQGDKFSEYFDANTYLLITRALDYFDPAGGHGGDLAAALARA-TAKFLLVS 311

Query: 318 FSSDWLYPTAENRHLVRALTAAGARAAFAEIESDKGHDAFLLDE 361
           FS+DW +    +R +V+AL       ++AEI++  GHDAFLLD+
Sbjct: 312 FSTDWRFSPQRSREIVKALLDNRRSVSYAEIDAPHGHDAFLLDD 355


Lambda     K      H
   0.321    0.135    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 451
Number of extensions: 16
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 382
Length of database: 374
Length adjustment: 30
Effective length of query: 352
Effective length of database: 344
Effective search space:   121088
Effective search space used:   121088
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 50 (23.9 bits)

Align candidate WP_038202407.1 Q392_RS05130 (homoserine O-acetyltransferase)
to HMM TIGR01392 (metX: homoserine O-acetyltransferase (EC 2.3.1.31))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01392.hmm
# target sequence database:        /tmp/gapView.607846.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01392  [M=351]
Accession:   TIGR01392
Description: homoserO_Ac_trn: homoserine O-acetyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   5.9e-142  458.8   0.0   6.7e-142  458.6   0.0    1.0  1  NCBI__GCF_000745855.1:WP_038202407.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000745855.1:WP_038202407.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  458.6   0.0  6.7e-142  6.7e-142       3     348 ..      14     364 ..      12     367 .. 0.97

  Alignments for each domain:
  == domain 1  score: 458.6 bits;  conditional E-value: 6.7e-142
                             TIGR01392   3 eeltlesGevlsevevayktyGtlnaerdNavlvcHaltgsahvagkadeedk..GWWdellGpgraldtsry 73 
                                            +l l sG+ l ++++ay+tyGtlnaer+NavlvcHal +s+hvag++  + k  GWWd+++Gpg+a+dt+r+
  NCBI__GCF_000745855.1:WP_038202407.1  14 APLALRSGASLGDYTLAYETYGTLNAERSNAVLVCHALNASHHVAGRYAGQPKseGWWDNMIGPGKAVDTDRF 86 
                                           5899********************************************9877788****************** PP

                             TIGR01392  74 fvvclNvlGsckGstgPlsinpetgkpygaefPlvtirDlvkaqkalldsLgveklaavvGgSlGGmqalewa 146
                                           fv+++N+lGsc GstgP+ +np+tg+ yga+fP+vt++D+v aq++lld+Lg+++laav+GgSlGGmqal w+
  NCBI__GCF_000745855.1:WP_038202407.1  87 FVIGINNLGSCFGSTGPMHVNPATGRVYGADFPVVTVEDWVDAQARLLDALGIDTLAAVMGGSLGGMQALSWT 159
                                           ************************************************************************* PP

                             TIGR01392 147 lsypervkkivvlatsarasaqaiafnevqrqailsDpeyndGeyaeee.qPekGLalARmlalltYrseesl 218
                                           l+yperv++++v+a++++++a++iafnev+r+ai +Dp++++G+++e++  P++GL++ARm++++tY+s++ +
  NCBI__GCF_000745855.1:WP_038202407.1 160 LQYPERVRHAAVIASAPNLNAENIAFNEVARRAIVTDPDFHGGHFYEHGvVPKRGLRIARMIGHITYLSDDVM 232
                                           *************************************************9*********************** PP

                             TIGR01392 219 eerfgreakseeslassleeefsvesylryqgkkfverFdAnsYllltkaldthdlargrrdslkealkkika 291
                                           +e+fgre ++     ++++ ef++esylryqg+kf e FdAn+Yll+t+ald++d a g+ ++l++al++++a
  NCBI__GCF_000745855.1:WP_038202407.1 233 NEKFGRELREGVVRYTTQDVEFQIESYLRYQGDKFSEYFDANTYLLITRALDYFDPAGGHGGDLAAALARATA 305
                                           ***********9888889******************************************************* PP

                             TIGR01392 292 pvlvvgiesDllftleeqeelakalkaakle..yaeieseeGHDaFllekekveelire 348
                                           ++l+v++++D++f++++++e++kal +++ +  yaei+ ++GHDaFll++ ++  ++r+
  NCBI__GCF_000745855.1:WP_038202407.1 306 KFLLVSFSTDWRFSPQRSREIVKALLDNRRSvsYAEIDAPHGHDAFLLDDVRYLGVVRA 364
                                           *************************99988888****************9887777665 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (351 nodes)
Target sequences:                          1  (374 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 30.80
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the original publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis