Alignments for a candidate for ilvD in Thiomicrospira microaerophila ASL8-2

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvD in Thiomicrospira microaerophila ASL8-2

Align Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 (characterized)
to candidate WP_044410082.1 NA59_RS06955 dihydroxy-acid dehydratase

Query= SwissProt::Q1MIB2
         (612 letters)



>NCBI__GCF_000934765.1:WP_044410082.1
          Length = 620

 Score =  925 bits (2391), Expect = 0.0
 Identities = 453/612 (74%), Positives = 521/612 (85%), Gaps = 3/612 (0%)

Query: 1   MPVYRSRTTTHGRNMAGARGLWRATGMKDSDFGKPIIAVVNSFTQFVPGHVHLKDLGQLV 60
           MP YRSRT+THGRNMAGAR LWRATGM   DFGKPIIA+ NSFTQFVPGHVHLKD+GQLV
Sbjct: 1   MPAYRSRTSTHGRNMAGARALWRATGMNTEDFGKPIIAIANSFTQFVPGHVHLKDMGQLV 60

Query: 61  AREIEAAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRELIADSVEYMVNAHCADAMVCIS 120
           AR +E AGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRE+IADSVEYMVNAHCADA+VCIS
Sbjct: 61  ARVVEQAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSREVIADSVEYMVNAHCADALVCIS 120

Query: 121 NCDKITPGMLMASLRLNIPTVFVSGGPMEAGKVVMHGKTHALDLVDAMVAAADDKISDED 180
           NCDKITPGMLMA++RLNIPT+FVSGGPME+GK ++ G    LDLVDAMV AADD +SD D
Sbjct: 121 NCDKITPGMLMAAMRLNIPTIFVSGGPMESGKSILGGVEIKLDLVDAMVMAADDNVSDAD 180

Query: 181 VQTIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSTLATHLDRKRLFVEAGHLIV 240
           V+ +E SACPTCGSCSGMFTANSMNCL EALG++LPGNGS LATH DRK LF EAG  IV
Sbjct: 181 VEAVEISACPTCGSCSGMFTANSMNCLAEALGMALPGNGSMLATHADRKHLFEEAGRRIV 240

Query: 241 DLARRYYEQDDVKALPRTIASKQAFENAMTLDIAMGGSTNTVLHILAAAHEGEIDFTMAD 300
           ++A+ YYE+D+   LPR+IA+  AF+NA+ LD+AMGGSTNTVLH+LA AHE ++ FTMAD
Sbjct: 241 EIAKDYYEKDNHNVLPRSIATFSAFQNAVALDVAMGGSTNTVLHLLAVAHEADVSFTMAD 300

Query: 301 IDALSRRVPCLSKVAPAKSDVHMEDVHRAGGIMSILGELDKGGLLNRDCPTVHAETLGDA 360
           IDA+SR+VPCLSKVAP     HMEDVHRAGGIM +LGELD+GGL++    TVHA TLG A
Sbjct: 301 IDAVSRKVPCLSKVAPNSKIYHMEDVHRAGGIMRLLGELDRGGLIDPTTYTVHASTLGAA 360

Query: 361 IDRWDITRTNSETVRKFYRAAPGGIPTQVAFSQEARWDELDTDRENGVIRSVEHPFSKDG 420
           I+ WDI+R  SE V+KFY AAPG + T  AFSQ+ RW  LD D +NG IRSVEH +++DG
Sbjct: 361 IELWDISREPSEAVQKFYSAAPGNVRTTQAFSQDKRWKTLDDDAQNGCIRSVEHAYTQDG 420

Query: 421 GLAVLKGNLAIDGCIVKTAGVDESILKFSGPARVFESQDASVKAILANEVKAGDVVVIRY 480
           GLAVL GN+A DGCIVKTAGVDE I KFSGPAR+FESQD++V  IL NEVKAG+VV+IRY
Sbjct: 421 GLAVLFGNIAQDGCIVKTAGVDEEIFKFSGPARIFESQDSAVAGILGNEVKAGEVVIIRY 480

Query: 481 EGPKGGPGMQEMLYPTSYLKSKGLGKACALITDGRFSGGTSGLSIGHASPEAANGGTIGL 540
           EGPKGGPGMQEMLYPTSYLKSKG+GKACAL+TDGRFSGGTSGLSIGHASPEAA GG IGL
Sbjct: 481 EGPKGGPGMQEMLYPTSYLKSKGMGKACALLTDGRFSGGTSGLSIGHASPEAAEGGNIGL 540

Query: 541 VREGDMIDIDIPNRTISLRVSETELAARRAEQDAKG---WYPTEVRKRNVTTALKAYAAF 597
           +  GD+I+IDIPNR+I+++VS+ ELA RR + + KG   W P E R+R+V+ AL+AYAA 
Sbjct: 541 IEPGDIIEIDIPNRSINVKVSDYELAQRRQKMEQKGDQAWQPAEKRQRHVSAALRAYAAM 600

Query: 598 ATSADRGAVRDL 609
            TSA  GAVRD+
Sbjct: 601 TTSAAFGAVRDV 612


Lambda     K      H
   0.318    0.134    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1185
Number of extensions: 33
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 612
Length of database: 620
Length adjustment: 37
Effective length of query: 575
Effective length of database: 583
Effective search space:   335225
Effective search space used:   335225
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

Align candidate WP_044410082.1 NA59_RS06955 (dihydroxy-acid dehydratase)
to HMM TIGR00110 (ilvD: dihydroxy-acid dehydratase (EC 4.2.1.9))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00110.hmm
# target sequence database:        /tmp/gapView.3913887.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00110  [M=543]
Accession:   TIGR00110
Description: ilvD: dihydroxy-acid dehydratase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   5.8e-250  816.3   6.4   6.7e-250  816.1   6.4    1.0  1  NCBI__GCF_000934765.1:WP_044410082.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000934765.1:WP_044410082.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  816.1   6.4  6.7e-250  6.7e-250       2     542 ..      18     611 ..      17     612 .. 0.99

  Alignments for each domain:
  == domain 1  score: 816.1 bits;  conditional E-value: 6.7e-250
                             TIGR00110   2 arallkatGlkdedlekPiiavvnsyteivPghvhlkdlaklvkeeieaaGgvakefntiavsDGiamgheGm 74 
                                           aral++atG++ ed++kPiia++ns+t++vPghvhlkd+++lv++ +e+aGgvakefntiav+DGiamgh+Gm
  NCBI__GCF_000934765.1:WP_044410082.1  18 ARALWRATGMNTEDFGKPIIAIANSFTQFVPGHVHLKDMGQLVARVVEQAGGVAKEFNTIAVDDGIAMGHDGM 90 
                                           79*********************************************************************** PP

                             TIGR00110  75 kysLpsreiiaDsvetvvkahalDalvvissCDkivPGmlmaalrlniPaivvsGGpmeagktklsekidlvd 147
                                           +ysLpsre+iaDsve++v+ah++Dalv+is+CDki+PGmlmaa+rlniP+i+vsGGpme+gk  l+   +++d
  NCBI__GCF_000934765.1:WP_044410082.1  91 LYSLPSREVIADSVEYMVNAHCADALVCISNCDKITPGMLMAAMRLNIPTIFVSGGPMESGKSILGGVEIKLD 163
                                           *****************************************************************99999*** PP

                             TIGR00110 148 vfeavgeyaagklseeeleeiersacPtagsCsGlftansmacltealGlslPgsstllatsaekkelakksg 220
                                           +++a++++a++++s++++e++e sacPt+gsCsG+ftansm+cl+ealG++lPg++++lat+a++k+l++++g
  NCBI__GCF_000934765.1:WP_044410082.1 164 LVDAMVMAADDNVSDADVEAVEISACPTCGSCSGMFTANSMNCLAEALGMALPGNGSMLATHADRKHLFEEAG 236
                                           ************************************************************************* PP

                             TIGR00110 221 krivelvkknik.......PrdiltkeafenaitldlalGGstntvLhllaiakeagvklslddfdrlsrkvP 286
                                           +rive+ k +++       Pr+i+t +af+na++ld+a+GGstntvLhlla+a+ea+v++++ d+d +srkvP
  NCBI__GCF_000934765.1:WP_044410082.1 237 RRIVEIAKDYYEkdnhnvlPRSIATFSAFQNAVALDVAMGGSTNTVLHLLAVAHEADVSFTMADIDAVSRKVP 309
                                           ***********99************************************************************ PP

                             TIGR00110 287 llaklkPsgkkv.iedlhraGGvsavlkeldkegllhkdaltvtGktlaetlekvkvlr.............. 344
                                           +l+k++P++k + +ed+hraGG++ +l+eld+ gl++  + tv  +tl+  +e  ++ r              
  NCBI__GCF_000934765.1:WP_044410082.1 310 CLSKVAPNSKIYhMEDVHRAGGIMRLLGELDRGGLIDPTTYTVHASTLGAAIELWDISRepseavqkfysaap 382
                                           **********999*******************************************9999************* PP

                             TIGR00110 345 .....................vdqdvirsldnpvkkegglavLkGnlaeeGavvkiagveedilkfeGpakvf 396
                                                                +++ +irs++++++++gglavL+Gn+a++G++vk+agv+e+i kf Gpa++f
  NCBI__GCF_000934765.1:WP_044410082.1 383 gnvrttqafsqdkrwktldddAQNGCIRSVEHAYTQDGGLAVLFGNIAQDGCIVKTAGVDEEIFKFSGPARIF 455
                                           *****************77666666************************************************ PP

                             TIGR00110 397 eseeealeailggkvkeGdvvviryeGPkGgPGmremLaPtsalvglGLgkkvaLitDGrfsGgtrGlsiGhv 469
                                           es++ a+++ilg +vk+G+vv+iryeGPkGgPGm+emL+Pts+l++ G+gk++aL+tDGrfsGgt+GlsiGh 
  NCBI__GCF_000934765.1:WP_044410082.1 456 ESQDSAVAGILGNEVKAGEVVIIRYEGPKGGPGMQEMLYPTSYLKSKGMGKACALLTDGRFSGGTSGLSIGHA 528
                                           ************************************************************************* PP

                             TIGR00110 470 sPeaaegGaialvedGDkikiDienrkldlevseeelaerrakakkkea..........revkgaLakyaklv 532
                                           sPeaaegG+i+l+e GD+i+iDi+nr ++++vs+ ela+rr+k+++k++          r+v+ aL++ya + 
  NCBI__GCF_000934765.1:WP_044410082.1 529 SPEAAEGGNIGLIEPGDIIEIDIPNRSINVKVSDYELAQRRQKMEQKGDqawqpaekrqRHVSAALRAYAAMT 601
                                           ***********************************************9999******9999************ PP

                             TIGR00110 533 ssadkGavld 542
                                           +sa  Gav+d
  NCBI__GCF_000934765.1:WP_044410082.1 602 TSAAFGAVRD 611
                                           ********97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (543 nodes)
Target sequences:                          1  (620 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 18.57
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis