GapMind for Amino acid biosynthesis

 

Protein WP_053767937.1 in Thermus aquaticus YT-1

Annotation: NCBI__GCF_001280255.1:WP_053767937.1

Length: 561 amino acids

Source: GCF_001280255.1 in NCBI

Candidate for 3 steps in Amino acid biosynthesis

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-isoleucine biosynthesis ilvI hi acetolactate synthase (subunit 2/2) (EC 2.2.1.6) (characterized) 53% 91% 569.3
L-isoleucine biosynthesis ilvI hi ilvB: acetolactate synthase, large subunit, biosynthetic type (EC 2.2.1.6) (TIGR00118) 100% 797.8
L-leucine biosynthesis ilvI hi acetolactate synthase (subunit 2/2) (EC 2.2.1.6) (characterized) 53% 91% 569.3
L-leucine biosynthesis ilvI hi ilvB: acetolactate synthase, large subunit, biosynthetic type (EC 2.2.1.6) (TIGR00118) 100% 797.8
L-valine biosynthesis ilvI hi acetolactate synthase (subunit 2/2) (EC 2.2.1.6) (characterized) 53% 91% 569.3
L-valine biosynthesis ilvI hi ilvB: acetolactate synthase, large subunit, biosynthetic type (EC 2.2.1.6) (TIGR00118) 100% 797.8

Sequence Analysis Tools

View WP_053767937.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MKGAEALLKALEREGVEVIFGHPGGAIMPTYDALYDSPIRHILVRHEQGGIHAATAYARA
SGRVGVVMATSGPGALNLVTGLADALMDSTPVVAITGNVPRALIGTDAFQEADVTGVTMP
ITKHNYLVQDVNEIPRVVKEAFHIASTGRPGPVLIDLPKDVQLAEFTGEFDVELDLPGYK
PTLRGHPKQIERALDALEKAERPILMVGGGAQHAHAELLAFAEKTGLPVITTLMGLGAFP
GNHPLWLGMPGMHGTVAANRAIHHADVILAIGLRFDDRVTGKVSRFAPHAHTIIHVDIDP
AEIGKVVRTHIPIVGDSRLVLKEMLKGAKPLRLAAWWRELEEWRTRYPLRYRPRPHLQAP
EVIRAFHEATGGNAIVTTGVGQHQMFAAQYFTVTRPRSFLTSGGLGTMGVGLPFAIGAQI
ARPEELVIDFDGDGSFQMTLQELATVVKYRLPVKVVILNNGYLGMVRQWQDLFHAKRYSE
VYLADSNPDFAKLAEAYGIKGVRVERKEDLKKGVEAVLNADGPVVAEFKVYHEEGVFPMI
PAGGAAEDMILEHPEEEEVGA

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory