Annotation: NCBI__GCF_001280255.1:WP_053767939.1
Length: 526 amino acids
Source: GCF_001280255.1 in NCBI
Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
---|---|---|---|---|---|---|---|---|---|
L-isoleucine biosynthesis | cimA | hi | (R)-citramalate synthase (EC 2.3.3.21) (characterized) | 51% | 98% | 492.3 | 2-isopropylmalate synthase (EC 2.3.3.13) | 30% | 213.4 |
L-isoleucine biosynthesis | cimA | hi | cimA: citramalate synthase (EC 2.3.1.182) (TIGR00977) | 98% | 672.3 | 2-isopropylmalate synthase (EC 2.3.3.13) | 30% | 213.4 | |
L-leucine biosynthesis | leuA | lo | Probable 2-isopropylmalate synthase; EC 2.3.3.13; Alpha-IPM synthase; Alpha-isopropylmalate synthase (uncharacterized) | 34% | 97% | 239.6 | (R)-citramalate synthase (EC 2.3.3.21) | 51% | 492.3 |
L-lysine biosynthesis | hcs | lo | Homocitrate synthase AksA; EC 2.3.3.14; (R)-homo(2)citrate synthase; EC 2.3.3.-; (R)-homo(3)citrate synthase; EC 2.3.3.- (uncharacterized) | 36% | 87% | 182.6 | (R)-citramalate synthase (EC 2.3.3.21) | 51% | 492.3 |
View WP_053767939.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
MVEILDTTLRDGTQGEGISLSVEDKVAIAKRLAAFGIHLIEGGWPGSNPKDAEFFARMKG VDLGESRLAAFGATRRKGLLPEEDPSVLALLEAETPVVVLFGKSWTLHVLEALETSLEEN LRMVEDTVAFFASRGRRVIYDAEHFFDGYKEDPGYALATLEAALKGGADTLVLCDTNGGT LPEEVYAVTKVVVERFPGVRVGIHPHNDADLAVANALAAVRAGATHVQGTINGYGERCGN LNLTSFLPNLVFKYHIPAIPPERLRGLKELSHFVDERANLTPNRRAPYVGESAFAHKAGV HVSAVLKNPRTYEHIPPEWVGNGRRFLVSDVSGRSNLLAKLQELGVDLSKEEAKRLLDEV KALEYEGYAFEGAEASFYVLAHRLKGGSLPFQVEGFSVFVHGAGLATAWAEATVRVRVGK TLEHTAAESPYGPVSALDRAFRKAVLGFYPELADVELADYKVRILSGREAGTNSGVRVMV EMKRGGERFSTVGASENILEASLKALTDGYAYALLYPVRQATPSAG
This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory