Alignments for a candidate for aspS2 in Roseburia faecis M72

GapMind for Amino acid biosynthesis

Alignments for a candidate for aspS2 in Roseburia faecis M72

Align aspartate-tRNAAsn ligase (EC 6.1.1.23) (characterized)
to candidate WP_022045592.1 M72_RS02360 aspartate--tRNA ligase

Query= BRENDA::Q9KDG1
         (595 letters)



>NCBI__GCF_001406815.1:WP_022045592.1
          Length = 599

 Score =  647 bits (1670), Expect = 0.0
 Identities = 318/585 (54%), Positives = 434/585 (74%), Gaps = 4/585 (0%)

Query: 4   RTHHCGQLSEEQVNERVQLKGWVQRRRDLGQVIFVDLRDRSGVVQLVFNSD-ISQEALET 62
           R+H C ++S   + E+V + GWVQ+RR+LG +IF+DLRDRSG++Q+VF+ + +  E    
Sbjct: 10  RSHRCTEVSSANIGEKVTVMGWVQKRRNLGSLIFIDLRDRSGLLQIVFDEESVGAEGFAK 69

Query: 63  AEKVRNEYVLDVEGVVLKRDPSTVNDKIATGTIEVHVERLTILNKAKSLPFQIEANTDAS 122
           A  +R+EYV+ VEG V KR  + VN+ + TG IEV    L IL+++++ PF IE N+   
Sbjct: 70  AGTLRSEYVVAVEGTVQKRS-AAVNENLKTGDIEVIATSLRILSESQTPPFAIEENSQTK 128

Query: 123 EDIRLKYRYLDLRRPDMQETMKLRHQTTKLIRDFLDGQEFFEIETPMLTKSTPEGARDYL 182
           EDIRLKYRYLDLRRPD+Q+ + L+ +   L+R F+  + F EIETP+L KSTPEGARDYL
Sbjct: 129 EDIRLKYRYLDLRRPDIQKNLMLKSRVLGLMRQFMANEGFLEIETPILCKSTPEGARDYL 188

Query: 183 VPSRVHHGEFYALPQSPQIFKQLLMVSGFERYYQIVRCFRDEDLRADRQPEFTQIDIETS 242
           VPSRVH G FYALPQSPQ+FKQLLM SG++RY+QI RCFRDEDLRADRQPEFTQ D+E S
Sbjct: 189 VPSRVHPGNFYALPQSPQLFKQLLMASGYDRYFQIARCFRDEDLRADRQPEFTQADMELS 248

Query: 243 FMDKEDLLTMTENMMAKIMKEVKGLDVALPFPRMTYDDAMNRYGSDKPDTRFEMELIELS 302
           F+D +D++ + E ++  + KE  G+DV +P  RM + +AM+R+GSDKPDTRF MEL+++S
Sbjct: 249 FVDIDDVIDVNERLLKFVFKEAIGVDVQIPLQRMPWQEAMDRFGSDKPDTRFGMELVDVS 308

Query: 303 DIVKDSDFKVFSSAIKSGGIVKGLNLKGGAGSLSRKEIDGLAEFVKPYGAKGLAWLKV-E 361
           + VK   F VF+ A+++GG V+G+N++ G G + RK+ID L E  K  GAKGLA+L + E
Sbjct: 309 ETVKGCGFGVFTGALENGGSVRGINVE-GQGHMPRKKIDKLVEHAKGCGAKGLAYLCINE 367

Query: 362 EGELKGPIAKFFAGETGAELQQAMGAEDGDLLFFAADKKEVVFDSLGALRLKLGKDFNLI 421
           +G  K   AKF   +    L   M  + GDLL FAADK ++V++ LGALRL+LG++  LI
Sbjct: 368 DGTYKSSFAKFMTEDELNALVAKMNGKPGDLLLFAADKNKIVWNVLGALRLQLGEELGLI 427

Query: 422 DESKFNFLWVVDFPLVEYDEEAKRFVALHHPFTSPKQEDLTKLETDPASVRADAYDLVLN 481
           DE+K+NFLWV +FPL+E+ +E  RF+A+HHPFT P +ED  K+++DP +VRA AYD+VLN
Sbjct: 428 DENKYNFLWVTEFPLLEWSDEENRFMAMHHPFTMPMEEDWDKIDSDPGAVRAKAYDIVLN 487

Query: 482 GYELGGGSQRIYQRPVQEKMFAALGFTEEAAQKEFGFLLEAFEYGTPPHGGIALGLDRLV 541
           G ELGGGS RI+Q  +QEKMF  LGFT+E A ++FGFLL+AF YG PPH G+A G+DR++
Sbjct: 488 GTELGGGSVRIHQDDIQEKMFEVLGFTKERAHEQFGFLLDAFSYGVPPHAGLAYGVDRMI 547

Query: 542 MLLAGRLNLRDTIAFPKTASASCLLTEAPGEVSLEQLLDLNLSII 586
           M +    ++RD IAFPK   AS L++EAPG V  +QL +L ++I+
Sbjct: 548 MHMVHADSIRDVIAFPKVKDASDLMSEAPGSVDEKQLEELGIAIV 592


Lambda     K      H
   0.319    0.138    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 951
Number of extensions: 36
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 595
Length of database: 599
Length adjustment: 37
Effective length of query: 558
Effective length of database: 562
Effective search space:   313596
Effective search space used:   313596
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis