GapMind for Amino acid biosynthesis

 

Alignments for a candidate for leuD in Stenotrophomonas chelatiphaga DSM 21508

Align 3-isopropylmalate dehydratase small subunit; EC 4.2.1.33 (characterized, see rationale)
to candidate WP_057509304.1 ABB28_RS14505 3-isopropylmalate dehydratase small subunit

Query= uniprot:Q845W4
         (216 letters)



>NCBI__GCF_001431535.1:WP_057509304.1
          Length = 192

 Score =  171 bits (432), Expect = 1e-47
 Identities = 91/202 (45%), Positives = 114/202 (56%), Gaps = 13/202 (6%)

Query: 1   MEKFTVHTGVVAPLDRENVDTDAIIPKQFLKSIKRTGFGPNAFDEWRYLDHGEPGQDNSK 60
           M  F         L + N+DTD IIP +FL + +R G G NAF++WR+   G P      
Sbjct: 1   MSGFRTLASASVVLRQTNIDTDQIIPARFLSTTERAGLGRNAFNDWRWTSDGTP------ 54

Query: 61  RPLNPDFVLNQPRYQGASILVTRKNFGCGSSREHAPWALQQYGFRAIIAPSFADIFFNNC 120
              N DF  NQP+  G SIL+  +NFGCGSSREHAPWAL   G RAI++   ADIF  N 
Sbjct: 55  ---NADFAFNQPQNAGRSILLAGRNFGCGSSREHAPWALTDLGLRAIVSSEIADIFRGNS 111

Query: 121 FKNGLLPIVLTEQQVDHLINETVAFNGYQLTIDLEAQVVRTPDGRDYPFEITAFRKYCLL 180
            KNGLLP+VL E  V  L+          LTID+ A+ +RTPDGR Y F +  F + CLL
Sbjct: 112 LKNGLLPVVLDEADVQALMQRP----DDVLTIDIAARELRTPDGRVYTFPLDGFSQTCLL 167

Query: 181 NGFDDIGLTLRHADKIRQFEAE 202
            G D +G  L     I ++E E
Sbjct: 168 EGVDQLGYLLGRVPDIERYETE 189


Lambda     K      H
   0.322    0.141    0.437 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 149
Number of extensions: 4
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 216
Length of database: 192
Length adjustment: 21
Effective length of query: 195
Effective length of database: 171
Effective search space:    33345
Effective search space used:    33345
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 45 (21.9 bits)

Align candidate WP_057509304.1 ABB28_RS14505 (3-isopropylmalate dehydratase small subunit)
to HMM TIGR00171 (leuD: 3-isopropylmalate dehydratase, small subunit (EC 4.2.1.33))

Software error:

../bin/blast/fastacmd -i /tmp/list.31095.in -d ../tmp/orgsDef_201/orgs.faa -p T > /tmp/gapView.31095.genome.faa failed: Inappropriate ioctl for device at ../lib/pbutils.pm line 379.

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