Alignments for a candidate for serC in Steroidobacter denitrificans DSM 18526

GapMind for Amino acid biosynthesis

Alignments for a candidate for serC in Steroidobacter denitrificans DSM 18526

Align phosphoserine transaminase (EC 2.6.1.52) (characterized)
to candidate WP_237392702.1 ACG33_RS07085 3-phosphoserine/phosphohydroxythreonine transaminase

Query= BRENDA::P23721
         (362 letters)



>NCBI__GCF_001579945.1:WP_237392702.1
          Length = 359

 Score =  425 bits (1093), Expect = e-124
 Identities = 210/359 (58%), Positives = 260/359 (72%), Gaps = 2/359 (0%)

Query: 3   QIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGKEFIQVAEEAEKDFRDLLN 62
           ++FNFS+GPA+LP EVL+QA+ E+ DW+G G SVME+SHRGK F+ +A +AE D R+LL 
Sbjct: 2   RVFNFSAGPAVLPLEVLEQARDEMLDWHGTGMSVMEMSHRGKAFVSIAAQAEADLRELLQ 61

Query: 63  VPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYWAASAIKEAKKYCTPNVFDAK 122
           VP+NYKVLF  GG   QFAA+P+N+ G  +  DYV  G W+  AI EAK+Y   NV  A 
Sbjct: 62  VPANYKVLFLQGGATAQFAAIPMNLAGPDSVVDYVHTGAWSKKAIGEAKRYANVNV--AA 119

Query: 123 VTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAIDETPDFGADVVVAADFSSTILSR 182
              +   ++     WQ S NAAY+HY PNETI G+     PD  A+V + AD SSTILSR
Sbjct: 120 NAGEPYASIPAQESWQRSPNAAYLHYTPNETIGGVEFHFIPDTNAEVPLVADLSSTILSR 179

Query: 183 PIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKANIACPSILDYSILNDNGSMFNTPP 242
           P+DVS++G+IYAGAQKNIGPAGL +VIVREDLLG+A    P+ILD++ +  +GSM NTPP
Sbjct: 180 PLDVSKFGLIYAGAQKNIGPAGLALVIVREDLLGRARAGTPTILDFAAMAKDGSMLNTPP 239

Query: 243 TFAWYLSGLVFKWLKANGGVAEMDKINQQKAELLYGVIDNSDFYRNDVAKANRSRMNVPF 302
           T+AWY++GLVF+WL+  GG+  M K NQ KA  LY  ID+S FY N VAK  RS MNVPF
Sbjct: 240 TYAWYVAGLVFQWLRRQGGLEAMGKRNQAKAAKLYEAIDDSSFYANPVAKGARSWMNVPF 299

Query: 303 QLADSALDKLFLEESFAAGLHALKGHRVVGGMRASIYNAMPLEGVKALTDFMVEFERRH 361
            LA   LDK F+ E+  AGL  L+GHR VGGMRAS+YNAMP+EGV AL  FM EFERRH
Sbjct: 300 TLARPELDKTFIAEAREAGLVTLEGHRSVGGMRASLYNAMPMEGVDALVGFMREFERRH 358


Lambda     K      H
   0.319    0.136    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 422
Number of extensions: 14
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 362
Length of database: 359
Length adjustment: 29
Effective length of query: 333
Effective length of database: 330
Effective search space:   109890
Effective search space used:   109890
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

Align candidate WP_237392702.1 ACG33_RS07085 (3-phosphoserine/phosphohydroxythreonine transaminase)
to HMM TIGR01364 (serC: phosphoserine transaminase (EC 2.6.1.52))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01364.hmm
# target sequence database:        /tmp/gapView.2658997.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01364  [M=358]
Accession:   TIGR01364
Description: serC_1: phosphoserine transaminase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
     1e-160  520.4   0.0   1.2e-160  520.3   0.0    1.0  1  NCBI__GCF_001579945.1:WP_237392702.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_001579945.1:WP_237392702.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  520.3   0.0  1.2e-160  1.2e-160       1     358 []       3     358 ..       3     358 .. 0.98

  Alignments for each domain:
  == domain 1  score: 520.3 bits;  conditional E-value: 1.2e-160
                             TIGR01364   1 ivnFsaGPaalpeevlekaqkelldfnglglsvmeisHRskefekvveeaesdlreLlnipdnyevlflqGGa 73 
                                           ++nFsaGPa+lp evle+a++e+ld++g+g+svme+sHR k+f +++ +ae dlreLl++p ny+vlflqGGa
  NCBI__GCF_001579945.1:WP_237392702.1   3 VFNFSAGPAVLPLEVLEQARDEMLDWHGTGMSVMEMSHRGKAFVSIAAQAEADLRELLQVPANYKVLFLQGGA 75 
                                           58*********************************************************************** PP

                             TIGR01364  74 ttqfaavplnllkekkvadyivtGawskkalkeakkltkevkvvaseeekkyskipdeeelelkedaayvylc 146
                                           t qfaa+p+nl+  ++v+dy+ tGawskka+ eak++ + v+v+a++  + y +ip +e+++ +++aay++++
  NCBI__GCF_001579945.1:WP_237392702.1  76 TAQFAAIPMNLAGPDSVVDYVHTGAWSKKAIGEAKRYAN-VNVAANA-GEPYASIPAQESWQRSPNAAYLHYT 146
                                           *************************************98.9988887.689********************** PP

                             TIGR01364 147 anetieGvefkelpevkk.aplvaDlssdilsrkidvskygliyaGaqKniGpaGvtvvivrkdllerakkel 218
                                            neti Gvef+ +p+++  +plvaDlss ilsr++dvsk+gliyaGaqKniGpaG+++vivr+dll+ra++ +
  NCBI__GCF_001579945.1:WP_237392702.1 147 PNETIGGVEFHFIPDTNAeVPLVADLSSTILSRPLDVSKFGLIYAGAQKNIGPAGLALVIVREDLLGRARAGT 219
                                           **************876559***************************************************** PP

                             TIGR01364 219 psvldYkilaendslyntpptfaiyvlglvlkwlkekGGvkklekknqeKakllYeaidesegfyknkvekka 291
                                           p++ld++ +a+  s++ntppt+a+yv+glv++wl+++GG++++ k+nq+Ka+ lYeaid+s+ fy n+v+k a
  NCBI__GCF_001579945.1:WP_237392702.1 220 PTILDFAAMAKDGSMLNTPPTYAWYVAGLVFQWLRRQGGLEAMGKRNQAKAAKLYEAIDDSS-FYANPVAKGA 291
                                           ***********************************************************996.********** PP

                             TIGR01364 292 RslmnvvFtlkkeelekeFlkeaeekglvslkGhrsvGGiRasiYnalpleevqaLvdfmkeFekkh 358
                                           Rs+mnv+Ftl+  el+k+F++ea+e+glv+l GhrsvGG+Ras+Yna+p+e+v aLv fm+eFe++h
  NCBI__GCF_001579945.1:WP_237392702.1 292 RSWMNVPFTLARPELDKTFIAEAREAGLVTLEGHRSVGGMRASLYNAMPMEGVDALVGFMREFERRH 358
                                           ****************************************************************987 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (358 nodes)
Target sequences:                          1  (359 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 20.87
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis