Alignments for a candidate for split_metE_2 in Steroidobacter denitrificans DSM 18526

GapMind for Amino acid biosynthesis

Alignments for a candidate for split_metE_2 in Steroidobacter denitrificans DSM 18526

Align Predicted split_metE_2 by GapMind curators (no experimental data)
to candidate WP_066918374.1 ACG33_RS02345 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase

Query= predicted:G0EFB7_PYRF1
         (339 letters)



>NCBI__GCF_001579945.1:WP_066918374.1
          Length = 764

 Score =  165 bits (417), Expect = 4e-45
 Identities = 104/335 (31%), Positives = 174/335 (51%), Gaps = 15/335 (4%)

Query: 8   RLPKAFITTVVGSYPKMPEAEEAIRKRRQGLITEEEFHKLVQPAIKKVIEDHLEAGVDIL 67
           +LPK F TT +GS+P+ PE  +A  + + G I +  +HK ++  I++ + +    G+D+ 
Sbjct: 431 KLPK-FPTTTIGSFPQTPEVRQARSRFKAGRIDQAAYHKAMRAEIERSVREQESLGLDVF 489

Query: 68  SDGEQARDDMVVYFAERIGGYSGGE--WVRIFDNVYFRKPVIVARLEYKGPMAVMDWEYA 125
             GE  R+DMV YF E++ GY+     WV+ + +   + P++   +     M V   +YA
Sbjct: 490 VHGEAERNDMVEYFGEQLDGYAFTRFGWVQSYGSRCVKPPILFGDISRPKAMTVEWIKYA 549

Query: 126 TSIAQGRPVKAILTGPYTMADWSFDLQYGDRRELVLELARVLRHEIEEFVKRGAKFIQVD 185
            S+   +P+K +LTGP T+ +WSF      R     +LA  +R E+ +  K G + IQ+D
Sbjct: 550 ASLTD-KPMKGMLTGPVTLLNWSFVRDDQPRSVSCKQLALAIRAEVLDLEKAGIRIIQID 608

Query: 186 EPA----LPTRPLPEEAEL--VKEALEIMLKGI--EVKKIVHVCFGRIEKLLPHLMEFPV 237
           E A    LP R    ++ L    EA  I   G+  E +   H+C+     ++  + +   
Sbjct: 609 EAALREGLPLRKSQWKSYLSWAVEAFRIAANGVADETQIHTHMCYSEFNDIIASIADMDA 668

Query: 238 DQLDLEFKNSGFKLLPYVKEYWDPRKEIGYGVIDVHSLRVESVEEIIEDVERLLKLDVIP 297
           D + +E   S  +LL    ++  P  EIG GV D+HS  +   E I++ + +    + +P
Sbjct: 669 DVITIETSRSAMELLDAFDDFRYP-NEIGPGVYDIHSPNIPRREHIVQLMRK--AAERVP 725

Query: 298 PEKIYIDPDCGLKRLPRDVARAKLRNMVEAARQLR 332
            E+++++PDCGLK          L NMV AAR+LR
Sbjct: 726 AERLWVNPDCGLKTRQWSEVIPALANMVAAARELR 760



 Score = 53.5 bits (127), Expect = 2e-11
 Identities = 53/233 (22%), Positives = 104/233 (44%), Gaps = 8/233 (3%)

Query: 86  GGYSGGEWVRIFD-NVYFRKPVIVARLEYKGPMAVMDWEYATSIAQGRPVKAILTGPYTM 144
           GG + GE  + FD N ++  P   A+ E++   + +  + A + +QG   K ++ GP T 
Sbjct: 112 GGVAAGEMTKWFDTNYHYIVPEFDAQSEFRLDASRLLVQLAEARSQGINAKPVIIGPITY 171

Query: 145 ADWSFDLQYGDRRELVLELARVLRHEIEEFVKRGAKFIQVDEPALPTRPLPEEAELVKEA 204
                     D+  L+  L  V +  +     +   ++Q+DEP L T       +    A
Sbjct: 172 LALGKAKDDSDKLALLPRLLPVYQELLTALAAQSVAWVQIDEPILVTELEDRWRQAYISA 231

Query: 205 LEIMLKGIEVKKIVHVCFGRIEKLLPHLMEFPVDQLDLEFKNSGFKLLPYVKEYWDPRKE 264
              +  G +VK ++   FG + + L  +   PV  + L+   +  ++ P V       + 
Sbjct: 232 YAALNTG-KVKLLLATYFGPLLENLSLVGALPVQGIHLDAIRARQEVDPLVARL-PADRI 289

Query: 265 IGYGVIDVHSLRVESVEEIIEDVERL-LKLDVIPPEKIYIDPDCGLKRLPRDV 316
           +  GVID  ++    +  +++ +E L +KL     ++++I P C L  +P D+
Sbjct: 290 VSLGVIDGRNIWKTDINALLDWLEPLAMKLG----DRLWIAPSCSLLHVPVDL 338


Lambda     K      H
   0.321    0.141    0.414 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 560
Number of extensions: 28
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 339
Length of database: 764
Length adjustment: 34
Effective length of query: 305
Effective length of database: 730
Effective search space:   222650
Effective search space used:   222650
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the original publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis