Alignments for a candidate for cmutase in Steroidobacter denitrificans DSM 18526

GapMind for Amino acid biosynthesis

Alignments for a candidate for cmutase in Steroidobacter denitrificans DSM 18526

Align Bifunctional chorismate mutase/prephenate dehydratase; Chorismate mutase-prephenate dehydratase; P-protein; EC 5.4.99.5; EC 4.2.1.51 (characterized)
to candidate WP_083536533.1 ACG33_RS07095 prephenate dehydratase

Query= SwissProt::P27603
         (365 letters)



>NCBI__GCF_001579945.1:WP_083536533.1
          Length = 414

 Score =  399 bits (1024), Expect = e-115
 Identities = 205/364 (56%), Positives = 267/364 (73%), Gaps = 5/364 (1%)

Query: 4   ADQLKALRVRIDSLDERILDLISERARCAQEVARVKTASWPKAEEAVFYRPEREAWVLKH 63
           AD L  +R RID +D +I +L++ERARCAQ+V R K ++   A +  FYRPEREA VL+ 
Sbjct: 54  ADGLDCIRERIDDIDCQIHELLNERARCAQQVGRSKRSAGLSAVD--FYRPEREAQVLRR 111

Query: 64  IMELNKGPLDNEEMARLFREIMSSCLALEQPLRVAYLGPEGTFSQAAALKHFGHSVISKP 123
           ++E NKGPL NEE+ RLFREIMS+CLA ++PL++A+LGPEGTFSQ A LKHFGHSV + P
Sbjct: 112 VIERNKGPLRNEEVVRLFREIMSACLAQQEPLKIAFLGPEGTFSQQAVLKHFGHSVRALP 171

Query: 124 MAAIDEVFREVVAGAVNFGVVPVENSTEGAVNHTLDSFLEHDIVICGEVELRIHHHLLVG 183
           + AI+EVF+EV +G  +FGVVP+ENSTEG VNHTLD F    + ICGEVELRI  HL+ G
Sbjct: 172 LPAINEVFQEVQSGHADFGVVPIENSTEGTVNHTLDMFPSSPLKICGEVELRIQQHLM-G 230

Query: 184 ETTKTDRITRIYSHAQSLAQCRKWLDAHYPNVERVAVSSNADAAKRVKSEWNSAAIAGDM 243
             +  + I R+ SH QSLAQCR+WLD + PN ER+ V+SNA+AA+R + E  +AAIAG  
Sbjct: 231 RMSGLENIARVCSHQQSLAQCRQWLDEYLPNAERMPVASNAEAARRARDEDGTAAIAGQA 290

Query: 244 AAQLYGLSKLAEKIEDRPVNSTRFLIIGSQEVPPTGDDKTSIIVSM--RNKPGALHELLM 301
           AA++YGL+ L   IED P N+TRFL+IG +    +G+DKT+++VS      PGALH+LL 
Sbjct: 291 AAEVYGLNILVPDIEDSPDNTTRFLVIGRKLFSASGNDKTTLLVSAGDTQAPGALHQLLG 350

Query: 302 PFHSNGIDLTRIETRPSRSGKWTYVFFIDCMGHHQDPLIKNVLEKIGHEAVALKVLGSYP 361
           P   + I +TRIE+RPSR  KW YVFFID  GH  +P +K  LE++  +A   ++LGSYP
Sbjct: 351 PLARHDITMTRIESRPSRRRKWDYVFFIDVAGHADEPPLKQALEELRKQASLFRILGSYP 410

Query: 362 KAVL 365
            AVL
Sbjct: 411 CAVL 414


Lambda     K      H
   0.319    0.133    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 418
Number of extensions: 14
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 365
Length of database: 414
Length adjustment: 30
Effective length of query: 335
Effective length of database: 384
Effective search space:   128640
Effective search space used:   128640
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

Align candidate WP_083536533.1 ACG33_RS07095 (prephenate dehydratase)
to HMM TIGR01807 (pheA: chorismate mutase (EC 5.4.99.5))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01807.hmm
# target sequence database:        /tmp/gapView.3383882.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01807  [M=76]
Accession:   TIGR01807
Description: CM_P2: chorismate mutase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
    9.4e-32   95.4   3.1      3e-31   93.8   1.1    2.5  3  NCBI__GCF_001579945.1:WP_083536533.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_001579945.1:WP_083536533.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !   93.8   1.1     3e-31     3e-31       3      76 .]      59     133 ..      57     133 .. 0.94
   2 ?   -3.1   0.0      0.53      0.53      63      75 ..     170     182 ..     157     182 .. 0.73
   3 ?    0.2   0.0     0.051     0.051      13      37 ..     222     246 ..     218     253 .. 0.76

  Alignments for each domain:
  == domain 1  score: 93.8 bits;  conditional E-value: 3e-31
                             TIGR01807   3 elRnkiDaiDdrildLlseRaklakavgelKkks.aseaviYRPeREaavlrrlkelnkGpLdqeavarifrE 74 
                                            +R++iD iD +i++Ll+eRa++a++vg+ K+++  s   +YRPeREa+vlrr+ e+nkGpL +e+v r+frE
  NCBI__GCF_001579945.1:WP_083536533.1  59 CIRERIDDIDCQIHELLNERARCAQQVGRSKRSAgLSAVDFYRPEREAQVLRRVIERNKGPLRNEEVVRLFRE 131
                                           68*****************************998456677********************************* PP

                             TIGR01807  75 im 76 
                                           im
  NCBI__GCF_001579945.1:WP_083536533.1 132 IM 133
                                           *9 PP

  == domain 2  score: -3.1 bits;  conditional E-value: 0.53
                             TIGR01807  63 LdqeavarifrEi 75 
                                           L+  a++ +f+E+
  NCBI__GCF_001579945.1:WP_083536533.1 170 LPLPAINEVFQEV 182
                                           6667899999997 PP

  == domain 3  score: 0.2 bits;  conditional E-value: 0.051
                             TIGR01807  13 drildLlseRaklakavgelKkksa 37 
                                            ri + l+ R+   ++++++  ++ 
  NCBI__GCF_001579945.1:WP_083536533.1 222 LRIQQHLMGRMSGLENIARVCSHQQ 246
                                           5788889999999999999877754 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (76 nodes)
Target sequences:                          1  (414 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 18.12
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis