Finding step split_metE_2 for L-methionine biosynthesis in Hydrogenophaga taeniospiralis CCUG 15921 NBRC 102512
No candidates for split_metE_2: vitamin B12-independent methionine synthase, catalytic component
GapMind classifies a step as low confidence even if it does not find any candidates. You can still try to find candidates by using Curated BLAST (which searches the 6-frame translation) or by text search of the annotations (which may indicate weak homology, under 30% identity or 50% coverage, that GapMind does not consider). See the links below.
Definition of step split_metE_2
- Predicted: UniProt sequence G0EFB7_PYRF1: RecName: Full=Methionine synthase {ECO:0000256|HAMAP-Rule:MF_00288}; EC=2.1.1.- {ECO:0000256|HAMAP-Rule:MF_00288}; AltName: Full=Homocysteine methyltransferase {ECO:0000256|HAMAP-Rule:MF_00288};
- Predicted: UniProt sequence D4GW90: RecName: Full=Methionine synthase {ECO:0000256|HAMAP-Rule:MF_00288}; EC=2.1.1.- {ECO:0000256|HAMAP-Rule:MF_00288}; AltName: Full=Homocysteine methyltransferase {ECO:0000256|HAMAP-Rule:MF_00288};
- Comment: In many thermophilic archaea, MetE seems to be split into two pieces (see PMC7857596). The catalytic component has the necessary zinc-binding residues (H219, C221, C307) and homocysteine-binding residues (S20, E71, D185). We added the gene from Haloferax volcanii (D4GW90) as it has the correct functional residues and is conserved next to a potential folate-binding component (D4GW90).
Or cluster all characterized split_metE_2 proteins
This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory