Alignments for a candidate for gatA in Brevundimonas sp. GW460-12-10-14-LB2

GapMind for Amino acid biosynthesis

Alignments for a candidate for gatA in Brevundimonas sp. GW460-12-10-14-LB2

Align Glutamyl-tRNA(Gln) amidotransferase subunit A; Glu-ADT subunit A; EC 6.3.5.7 (uncharacterized)
to candidate WP_197458471.1 A4249_RS06535 amidase

Query= curated2:A7NKM0
         (490 letters)



>NCBI__GCF_001636925.1:WP_197458471.1
          Length = 497

 Score =  201 bits (512), Expect = 4e-56
 Identities = 158/445 (35%), Positives = 219/445 (49%), Gaps = 34/445 (7%)

Query: 61  ARRAAGDASPLLGIPMGIKDVISTQGLRTTCASKMLENYTPVYDATAVARLKAAGAVILG 120
           AR      S + G+P+ +KD I T  + TT  S  L N  P  DA  V RL+AAGAVI+G
Sbjct: 66  ARSPRQGVSAISGMPILLKDNIETADMPTTAGSLALANNAPGRDAPLVTRLRAAGAVIVG 125

Query: 121 KLNCDEFAMGSSTEN-----SAFQQTRNPWNLERVPGGSSGGSAAAVAAGEAPAALGTDT 175
           K N  E+A   S+++     +   QT NP++  R P GSS GS AAVA G  P A+GT+T
Sbjct: 126 KTNLSEWANIRSSDSISGWSAVGGQTLNPYDPARTPCGSSSGSGAAVALGLTPVAIGTET 185

Query: 176 GGSIRQPAALCGITGLKPTYGRVSRYGLVAFASSLDQIGPMARTVRDCAIVLRVIAGADP 235
            GSI  PA++ G+ G KPT G VSR  +V  + S D  GPMA TVRD A VL VIAG+DP
Sbjct: 186 DGSITCPASVNGVVGFKPTVGLVSRTHIVPISHSQDTAGPMATTVRDAARVLTVIAGSDP 245

Query: 236 FDATCTDYPA--PDYEAALTGD-IRGLRIGVPREYFVAGMQPDVEAAVRTAIEVLREQGA 292
            D    +  A   DY A L  + +RG RIGV R  F+ G   + +A     ++ LR+ GA
Sbjct: 246 ADPATVEADAHKVDYAAGLDANALRGARIGVMR--FLKGYSSETQAVFEQNMKALRDAGA 303

Query: 293 EVCEISLPHTP---------YALPVYYLIAPAEASANLARFDGVRYGLRVPGESY-FDEL 342
            +  + LP  P         + + +Y L    + +A LA  D  +   R   +   F+  
Sbjct: 304 VL--VDLPEGPDGRVIGAAEFKVLLYEL--KHDLNAYLASTDPQQVRARALADVIAFNAA 359

Query: 343 ERTRGAGFGPEVRRRIMLGTYALSAGYYDAYYKRAQQVRTLIRRDYQQAFEQVDVIA--A 400
           E      FG ++  R         A Y +A   RA  +R        +      VIA  A
Sbjct: 360 EPRETVLFGQDIFERAQAMGDLTDADYLEA---RATSLRLAGPEGIDRLMRDNGVIALIA 416

Query: 401 PTTPTVAFKIGAHTDDPLAMYLEDVCTLPLNLAGLPGLVVPCGFAEGLPIGLQLIGRAFD 460
           PTT           DD     ++   +    +AG P L VP GF  G+P+G+  +G  +D
Sbjct: 417 PTTSRAWTNDPKDDDD-----MQGSASQLAAVAGYPHLTVPMGFDRGMPVGISFLGGKWD 471

Query: 461 EESLLRVGDAYQRVTDWHTRMPEVR 485
           +  +L +G A+++VT      P VR
Sbjct: 472 DARILSLGYAFEQVTQARRPPPGVR 496


Lambda     K      H
   0.320    0.136    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 532
Number of extensions: 26
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 490
Length of database: 497
Length adjustment: 34
Effective length of query: 456
Effective length of database: 463
Effective search space:   211128
Effective search space used:   211128
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis