Alignments for a candidate for proA in Thioalkalivibrio halophilus HL17

GapMind for Amino acid biosynthesis

Alignments for a candidate for proA in Thioalkalivibrio halophilus HL17

Align Gamma-glutamyl phosphate reductase; GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase; EC 1.2.1.41 (characterized)
to candidate WP_018946610.1 B1A74_RS11315 glutamate-5-semialdehyde dehydrogenase

Query= SwissProt::P07004
         (417 letters)



>NCBI__GCF_001995255.1:WP_018946610.1
          Length = 436

 Score =  380 bits (975), Expect = e-110
 Identities = 198/415 (47%), Positives = 274/415 (66%)

Query: 2   LEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILNANAQDVADARANGLSEA 61
           +E +G AA+ AS  +A+     KN  L+ IA+ +  +   +  AN +D+   RA GL  A
Sbjct: 21  IEALGRAARAASRHMAEAEPGAKNAALQAIAEGIAERRAELAEANERDLEAGRARGLDAA 80

Query: 62  MLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSGLRLERRRVPLGVIGVIYEA 121
           MLDRLALT AR+  + +  RQV  L DPVG + D     SG+++ + RVPLGVIG+IYE+
Sbjct: 81  MLDRLALTDARIDTMIEGCRQVAGLPDPVGTISDMAYQPSGIQVGQMRVPLGVIGIIYES 140

Query: 122 RPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQDALKSCGLPAGAVQAIDNPDR 181
           RPNVT+D A+LCLK+GNA ILRGG E   +N A   +IQ  L + GLP  AVQ +D  DR
Sbjct: 141 RPNVTIDAAALCLKSGNAAILRGGSEAAHSNRALAGIIQSGLAAAGLPREAVQIVDTTDR 200

Query: 182 ALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVITGGIGVCHIYVDESVEIAEALKV 241
           A V  +LRM +Y+D+++PRGG GL +   ++S IPVI    GVCH+YVD + +  +A  V
Sbjct: 201 AAVGALLRMPEYVDVIVPRGGKGLIERISQESQIPVIKHLDGVCHVYVDAAADPDKAQAV 260

Query: 242 IVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQMAESGVTLHADAAALAQLQAGPAKV 301
            VNAKT R  TCNT+ETLLV+ ++A++FLPA++ ++   GV+L   A   A L     +V
Sbjct: 261 AVNAKTHRYGTCNTMETLLVHADVAETFLPAVAAELDGLGVSLRGCARTRAVLADSGPEV 320

Query: 302 VAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIREHGTQHSDAILTRDMRNAQRFVNEVDS 361
                E++  E+L   L +++V DLD AI HI   G+ H+D+I+T +  +A+RF+  VDS
Sbjct: 321 AEASEEDWAAEYLGPILAIRVVDDLDAAIQHINTWGSHHTDSIVTENWGHARRFLRAVDS 380

Query: 362 SAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLEALTTYKWIGIGDYTIR 416
           S+V VNASTRF DG ++GLGAE+ +ST KLHARGP+GL  LTT K+I  GD  +R
Sbjct: 381 SSVMVNASTRFADGFEYGLGAEIGISTDKLHARGPVGLHGLTTLKYIVFGDGHVR 435


Lambda     K      H
   0.317    0.133    0.369 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 471
Number of extensions: 17
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 417
Length of database: 436
Length adjustment: 32
Effective length of query: 385
Effective length of database: 404
Effective search space:   155540
Effective search space used:   155540
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

Align candidate WP_018946610.1 B1A74_RS11315 (glutamate-5-semialdehyde dehydrogenase)
to HMM TIGR00407 (proA: glutamate-5-semialdehyde dehydrogenase (EC 1.2.1.41))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00407.hmm
# target sequence database:        /tmp/gapView.1023388.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00407  [M=398]
Accession:   TIGR00407
Description: proA: glutamate-5-semialdehyde dehydrogenase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   1.7e-157  510.2   0.1     2e-157  510.0   0.1    1.0  1  NCBI__GCF_001995255.1:WP_018946610.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_001995255.1:WP_018946610.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  510.0   0.1    2e-157    2e-157       1     398 []      28     425 ..      28     425 .. 0.99

  Alignments for each domain:
  == domain 1  score: 510.0 bits;  conditional E-value: 2e-157
                             TIGR00407   1 akeaalklaqlstaeknealskiadeLkaeaelilaanakdiaaakenGladalldrLlLteeklksiaddvk 73 
                                           a+ a+ ++a+     kn al++ia+++ ++  ++ +an++d++a+++ Gl  a+ldrL+Lt++++ +++++ +
  NCBI__GCF_001995255.1:WP_018946610.1  28 ARAASRHMAEAEPGAKNAALQAIAEGIAERRAELAEANERDLEAGRARGLDAAMLDRLALTDARIDTMIEGCR 100
                                           899********************************************************************** PP

                             TIGR00407  74 dvieLadPvGkvieareldeGLklervrvPlGvlgviyearPevivdvasLclktGnaviLkGgkeavrsnka 146
                                           +v+ L+dPvG + +     +G+++ ++rvPlGv+g+iye+rP+v++d+a+Lclk+Gna+iL+Gg+ea +sn+a
  NCBI__GCF_001995255.1:WP_018946610.1 101 QVAGLPDPVGTISDMAYQPSGIQVGQMRVPLGVIGIIYESRPNVTIDAAALCLKSGNAAILRGGSEAAHSNRA 173
                                           ************************************************************************* PP

                             TIGR00407 147 lveviqdaleqtglpveavqliedpdreevkellkldeyvdlliPrGgnelvklikeestiPvlehadGvChi 219
                                           l+ +iq+ l+ +glp eavq+++++dr+ v  ll++ eyvd+++PrGg++l++ i +es+iPv++h dGvCh+
  NCBI__GCF_001995255.1:WP_018946610.1 174 LAGIIQSGLAAAGLPREAVQIVDTTDRAAVGALLRMPEYVDVIVPRGGKGLIERISQESQIPVIKHLDGVCHV 246
                                           ************************************************************************* PP

                             TIGR00407 220 yldesadlakakkvivdaktqrPstCnaietLLvnkaiaeefleeLekqleekgvelradalvlkllelekat 292
                                           y+d+ ad  ka+ v v+akt+r  tCn++etLLv+ ++ae fl++++ +l   gv+lr+ a + ++l  ++ +
  NCBI__GCF_001995255.1:WP_018946610.1 247 YVDAAADPDKAQAVAVNAKTHRYGTCNTMETLLVHADVAETFLPAVAAELDGLGVSLRGCARTRAVLADSGPE 319
                                           ******************************************************************99999** PP

                             TIGR00407 293 eaevskedfdkeflsldLsvkivedleeaiehirqygtkhsdailtedkknaekfvkevdsaavyvnastrfa 365
                                           +ae+s+ed+  e+l ++L++++v+dl++ai+hi+++g++h+d+i+te+   a++f+++vds++v vnastrfa
  NCBI__GCF_001995255.1:WP_018946610.1 320 VAEASEEDWAAEYLGPILAIRVVDDLDAAIQHINTWGSHHTDSIVTENWGHARRFLRAVDSSSVMVNASTRFA 392
                                           ************************************************************************* PP

                             TIGR00407 366 dGfrfGfGaevgistqklharGPvGLeaLvsyk 398
                                           dGf++G+Gae+gist+klharGPvGL++L++ k
  NCBI__GCF_001995255.1:WP_018946610.1 393 DGFEYGLGAEIGISTDKLHARGPVGLHGLTTLK 425
                                           ******************************976 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (398 nodes)
Target sequences:                          1  (436 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 15.41
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the original publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis