Alignments for a candidate for gltX in Thioalkalivibrio denitrificans ALJD

GapMind for Amino acid biosynthesis

Alignments for a candidate for gltX in Thioalkalivibrio denitrificans ALJD

Align glutamyl-tRNA(Glx) synthetase (EC 6.1.1.24) (characterized)
to candidate WP_077278500.1 B1C78_RS07285 glutamate--tRNA ligase

Query= reanno::Caulo:CCNA_01982
         (470 letters)



>NCBI__GCF_002000365.1:WP_077278500.1
          Length = 470

 Score =  362 bits (930), Expect = e-104
 Identities = 200/465 (43%), Positives = 279/465 (60%), Gaps = 14/465 (3%)

Query: 9   VVTRFAPSPTGFLHIGGARTALFNWLYARHTGGKFLIRVEDTDRERSTEAAVAAIFEGLD 68
           V TRFAPSPTG+LHIGGARTALF+WLYAR  GG F++RVEDTD  RS   +V AI EG++
Sbjct: 3   VRTRFAPSPTGYLHIGGARTALFSWLYARKHGGDFVLRVEDTDLARSNAESVNAILEGMN 62

Query: 69  WLGLKSDDEVIFQHTRAPRHVEVVHELLAKGRAYRCWMSIEELEVAR-------EKARAE 121
           WLGL+ D+   +Q  R  R+ EVV  LL +G+AY C+ + EEL+  R       +K R +
Sbjct: 63  WLGLEYDEGPFYQTQRFERYREVVQRLLDEGKAYHCYCTKEELDAMRAEQMASKQKPRYD 122

Query: 122 GRAIRSPWRDAPEGDLSAPHVIRFKGPLDGETLVNDLVKGPVTFKNIELDDLVLLRADGA 181
           GR      R   E       V+RF+   DG T+V D+V+G V F+N ELDDL++ R+DG+
Sbjct: 123 GRC-----RHRTEPREGVEPVVRFRNADDGVTVVEDMVRGRVVFQNSELDDLIIARSDGS 177

Query: 182 PTYNLAVVVDDHDMGVTHVIRGDDHLNNAARQTLIYQAMDWAVPAFAHIPLIHGPDGAKL 241
           PTYNL VVVDD DM +THVIRGDDHLNN  RQ  I +A+D   P +AH+P+I GPDG KL
Sbjct: 178 PTYNLTVVVDDWDMQITHVIRGDDHLNNTPRQINILKALDVEPPKYAHLPMILGPDGTKL 237

Query: 242 SKRHGAQAVGEFADLGYIPEGMRNYLARLGWGHGDDEVFTDEQAISWFDVADVVKAPARL 301
           SKRHGA +V ++ D G++PE + NYL RLGW HGD EVFT E+ I  FD+ DV  + + +
Sbjct: 238 SKRHGAVSVMQYRDDGFLPEALINYLVRLGWSHGDQEVFTLEELIELFDIVDVNHSASAI 297

Query: 302 DWAKLNHINAQHLRKADDARLTALALAAAETRGEPLPADAAERIARTVPEVKEGAKTILE 361
           +  KL  +N  +++ +D   +           G  +   A   +   V   +E AKT+ E
Sbjct: 298 NPDKLLWLNQHYIKHSDPDHVARHLSWHMGNHG--IDPSAPPPLREVVTAQRERAKTLAE 355

Query: 362 LVDHCAFALKTRPLALEEKTQKQLTEETVERLKRLRDQLAAAPDFDAATLETVLKSFAES 421
           +  +  +  +      E+  +K LT +    L+ L   L A  D+ A  +   ++  AE 
Sbjct: 356 MAANSVYFYRDFEDYEEKAARKNLTGDARPVLQALHAALEALGDWTAEAIHAAVEGVAER 415

Query: 422 EGVGFGKFGPALRGVLTGGAQAPDLNKTMAALSRDEAIGRLDDAL 466
             +  GK    LR  +TGG+ +P +++T+A L +D  + R+  AL
Sbjct: 416 LELKLGKVAQPLRVAVTGGSVSPPIDQTLALLGKDRTLARIGRAL 460


Lambda     K      H
   0.319    0.135    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 655
Number of extensions: 32
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 470
Length of database: 470
Length adjustment: 33
Effective length of query: 437
Effective length of database: 437
Effective search space:   190969
Effective search space used:   190969
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis