GapMind for Amino acid biosynthesis

 

L-histidine biosynthesis in Alkalihalobacterium alkalinitrilicum DSM 22532

Best path

prs, hisG, hisI, hisE, hisA, hisF, hisH, hisB, hisC, hisN, hisD

Rules

Overview: Histidine biosynthesis in GapMind is based on the MetaCyc pathway (link).

11 steps (11 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
prs ribose-phosphate diphosphokinase BK574_RS17920
hisG ATP phosphoribosyltransferase BK574_RS20690
hisI phosphoribosyl-ATP pyrophosphatase BK574_RS20720 BK574_RS17885
hisE phosphoribosyl-AMP cyclohydrolase BK574_RS20720 BK574_RS20695
hisA 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase BK574_RS20710 BK574_RS20715
hisF imidazole glycerol phosphate synthase, cyclase subunit BK574_RS20715
hisH imidazole glycerol phosphate synthase, amidotransferase subunit BK574_RS20705 BK574_RS20715
hisB imidazoleglycerol-phosphate dehydratase BK574_RS20700
hisC histidinol-phosphate aminotransferase BK574_RS09880 BK574_RS10305
hisN histidinol-phosphate phosphatase BK574_RS22135 BK574_RS14305
hisD histidinal/histidinol dehydrogenase BK574_RS20695

Confidence: high confidence medium confidence low confidence
? – known gap: despite the lack of a good candidate for this step, this organism (or a related organism) performs the pathway

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory