Alignments for a candidate for hcs in Alkalihalobacterium alkalinitrilicum DSM 22532

GapMind for Amino acid biosynthesis

Alignments for a candidate for hcs in Alkalihalobacterium alkalinitrilicum DSM 22532

Align homocitrate synthase (EC 2.3.3.14) (characterized)
to candidate WP_078430192.1 BK574_RS22745 2-isopropylmalate synthase

Query= BRENDA::D0VY45
         (540 letters)



>NCBI__GCF_002019605.1:WP_078430192.1
          Length = 511

 Score =  413 bits (1061), Expect = e-120
 Identities = 232/514 (45%), Positives = 324/514 (63%), Gaps = 18/514 (3%)

Query: 25  VRILDTTLRDGEQSPGAAMTCVQKLETARQLAKLGVDIIEAGFPCASKQDFMAVKMIAEE 84
           + + DTTLRDGEQS G  +   +K+E A+QL +LGVD++EAGFP AS  DF +VK IA+ 
Sbjct: 4   INVFDTTLRDGEQSAGVNLNFEEKMEIAKQLERLGVDVMEAGFPAASPGDFNSVKAIADT 63

Query: 85  VGNCVDGNGYVPVITGVSRCNEKDIATAWEALKHAKRPRLRTFIATSPIHMEYKLRKSKD 144
           +  C         + G+SR  E DI  AW+ALK    PRL  FIATSPIHME+KLR + D
Sbjct: 64  IKGCS--------VIGLSRSVESDIDAAWDALKGGAEPRLHVFIATSPIHMEHKLRLTPD 115

Query: 145 QVLETARNMVKFARSLGCTDIQFGAEDAARSDKEFLYQIFGEVIKAGATTLTIPDTVGIA 204
           QV+E A   VK+A +     IQ+ AEDA RSD +FL +I  +VI AGA+ + IPDTVG  
Sbjct: 116 QVVENAVAAVKYAATR-FPKIQWSAEDACRSDLDFLVRIIEQVIDAGASVINIPDTVGYI 174

Query: 205 MPFEYGKLIADIKANTPGIENAIMATHCHNDLGLATANTIEGARYGARQLEVTINGIGER 264
            P E   + + +K N   ++ AI++THCH+DLGLATAN++     GA Q+E TINGIGER
Sbjct: 175 TPKEITHIFSYLKNNVRNMDKAILSTHCHDDLGLATANSLAAIEAGADQVECTINGIGER 234

Query: 265 AGNASFEEVVMALTCRGIDILGGLHTGINTRHILKTSKMVEKYSGLHLQPHKALVGANAF 324
           AGNAS EE+ +AL  R  +     +TGI  + I +TS +V K +G+ +  +KA+VG NAF
Sbjct: 235 AGNASLEEIAVALHIR--NDFYKANTGITLKEIKRTSSLVSKLTGMIVPNNKAVVGNNAF 292

Query: 325 LHESGIHQDGMLKHRGTYEIISPEDIGLVRSVGDTIVLGKLSGRQALRNRLEELGYKLKD 384
            HESGIHQDG+LK + TYEII+PE +G+     + +VLGK SGR A +++++ELGY   D
Sbjct: 293 AHESGIHQDGVLKEKSTYEIITPELVGV---SSNRMVLGKHSGRHAFKDKIKELGYTASD 349

Query: 385 TEVEGVFWQFKAVAEKKKRITDTDLRALVSNEAFNEQ-PIWKLGDLQVTCGTVGFSTATV 443
            ++  VF  FK +A+KKK IT+ D+ AL++ E        +++  LQV  GT    TAT+
Sbjct: 350 EQLNKVFKAFKELADKKKEITEDDIFALMTEEKIGAAVQYYEVDSLQVNYGTANIPTATI 409

Query: 444 KLFSIDGSMHVACSIGTGPVDSAYKAINHIVKEPAKLVKYTLGAITEGIDATATTSVEIS 503
            +   DG +    + G+G V++ Y  +  I+  P KL+ Y + +IT G DA A   V++ 
Sbjct: 410 TMKMPDGEVVQEAATGSGSVEAIYNTLERIIASPVKLLDYRIQSITGGRDALAEVYVKVR 469

Query: 504 RGDTNHPVFSGTGGGTDVVVSSVDAYLSALNNML 537
              T     SG G   DV+ +S  AY++A+N +L
Sbjct: 470 FDGTE---TSGRGTAHDVLEASARAYINAVNRVL 500


Lambda     K      H
   0.318    0.134    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 634
Number of extensions: 32
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 540
Length of database: 511
Length adjustment: 35
Effective length of query: 505
Effective length of database: 476
Effective search space:   240380
Effective search space used:   240380
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis