Alignments for a candidate for gatA in Thiohalospira halophila DSM 15071 HL 3

GapMind for Amino acid biosynthesis

Alignments for a candidate for gatA in Thiohalospira halophila DSM 15071 HL 3

Align Glutamyl-tRNA(Gln) amidotransferase subunit A; Glu-ADT subunit A; EC 6.3.5.7 (characterized)
to candidate WP_093428567.1 BM272_RS09595 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA

Query= SwissProt::O06491
         (485 letters)



>NCBI__GCF_900112605.1:WP_093428567.1
          Length = 489

 Score =  500 bits (1287), Expect = e-146
 Identities = 254/458 (55%), Positives = 334/458 (72%), Gaps = 6/458 (1%)

Query: 24  DLVDESYKRIQAVDDKVQAFLALDEERARAYAKELD-EAVDGRSEHGLLFGMPIGVKDNI 82
           +LV E+ +RI A  D   AF+ +D E ARA A+  D E   GR+  G L G+P+  KD  
Sbjct: 24  ELVTEALERIDA--DTHNAFITVDAEGARAAARAADAERAAGRA--GPLAGLPMAHKDIF 79

Query: 83  VTKGLRTTCSSKILENFDPIYDATVVQRLQDAEAVTIGKLNMDEFAMGSSTENSAYKLTK 142
            T GL+T+C S++L++F   YDATVV+R+  A  V +GK NMDEFAMGSS E S Y   +
Sbjct: 80  CTDGLKTSCGSRMLDSFVAPYDATVVERMAGAGLVNLGKTNMDEFAMGSSNETSYYGPVR 139

Query: 143 NPWNLDTVPGGSSGGSAAAVAAGEVPFSLGSDTGGSIRQPASFCGVVGLKPTYGRVSRYG 202
           NPW+   VPGGSSGGSAAAVAAG VP + G+DTGGSIRQPA+ CGV GLKPTYGRVSR+G
Sbjct: 140 NPWDPQAVPGGSSGGSAAAVAAGLVPGTTGTDTGGSIRQPAALCGVTGLKPTYGRVSRWG 199

Query: 203 LVAFASSLDQIGPITRTVEDNAFLLQAISGVDKMDSTSANVDVPDFLSSLTGDIKGLKIA 262
           ++AFASSLDQ GP+ RT ED A LL A++G D+ DSTS    VPD+   L   ++GL+I 
Sbjct: 200 MIAFASSLDQAGPMARTAEDCALLLGAMAGFDERDSTSLQEPVPDYTEGLGRSLEGLRIG 259

Query: 263 VPKEYLGEGVGKEARESVLAALKVLEGLGATWEEVSLPHSKYALATYYLLSSSEASANLA 322
           +PKEY G G+      ++ AA++   GLGA   EVSLPH++ A+ TYY+++ +E S+NL+
Sbjct: 260 LPKEYFGSGLDAGTARALEAAIEEFRGLGAEIREVSLPHAELAVPTYYVIAPAECSSNLS 319

Query: 323 RFDGIRYGYRTDNADNLIDLYKQTRAEGFGNEVKRRIMLGTFALSSGYYDAYYKKAQKVR 382
           RFDG+RYG+R  + ++L DLYK++R EGFG+EVKRR+M+GT+ALS+GY+DAYY KAQ++R
Sbjct: 320 RFDGVRYGHRCQDPEDLEDLYKRSRGEGFGDEVKRRVMVGTYALSAGYFDAYYLKAQRMR 379

Query: 383 TLIKKDFEDVFEKYDVIVGPTTPTPAFKIGENTKDPLTMYANDILTIPVNLAGVPGISVP 442
            LIK DF   FE+ D+++GPT P PAF IGE T DP++MY +DI TI  NLAG+P +SVP
Sbjct: 380 RLIKNDFMAAFEEVDLLLGPTAPGPAFNIGEKT-DPVSMYLSDIYTIAANLAGLPAMSVP 438

Query: 443 CGLADGLPLGLQIIGKHFDESTVYRVAHAFEQATDHHK 480
            GL DG P+GLQ+IG +FDE  +   AH ++Q TD H+
Sbjct: 439 AGLVDGRPVGLQLIGNYFDEPGLLNAAHRYQQVTDWHQ 476


Lambda     K      H
   0.315    0.134    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 620
Number of extensions: 24
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 485
Length of database: 489
Length adjustment: 34
Effective length of query: 451
Effective length of database: 455
Effective search space:   205205
Effective search space used:   205205
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

Align candidate WP_093428567.1 BM272_RS09595 (Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA)
to HMM TIGR00132 (gatA: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit (EC 6.3.5.-))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00132.hmm
# target sequence database:        /tmp/gapView.3606085.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00132  [M=466]
Accession:   TIGR00132
Description: gatA: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
     3e-181  589.2   0.0   3.4e-181  589.0   0.0    1.0  1  NCBI__GCF_900112605.1:WP_093428567.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_900112605.1:WP_093428567.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  589.0   0.0  3.4e-181  3.4e-181       3     465 ..      13     473 ..      11     474 .. 0.97

  Alignments for each domain:
  == domain 1  score: 589.0 bits;  conditional E-value: 3.4e-181
                             TIGR00132   3 ellkkkevsikevleeilerieavkdkinaflevtkekalkkakkldkkva.ke.kklagipiavKdniavkd 73 
                                           +++ + ++s +e+++e leri+a ++  naf++v++e a ++a++ d++ a  +  +lag+p+a Kd +++ +
  NCBI__GCF_900112605.1:WP_093428567.1  13 DAVAAGDCSGEELVTEALERIDADTH--NAFITVDAEGARAAARAADAERAaGRaGPLAGLPMAHKDIFCTDG 83 
                                           56777788899999999999998766..****************999988865537***************** PP

                             TIGR00132  74 iettcaSkiLenyvspydatVverlkeagaliiGktNlDEFamGsstetSafgvtknPeneervpGGSsgGsa 146
                                           ++t+c+S++L+ +v+pydatVver+  ag + +GktN+DEFamGss etS++g+++nP++ + vpGGSsgGsa
  NCBI__GCF_900112605.1:WP_093428567.1  84 LKTSCGSRMLDSFVAPYDATVVERMAGAGLVNLGKTNMDEFAMGSSNETSYYGPVRNPWDPQAVPGGSSGGSA 156
                                           ************************************************************************* PP

                             TIGR00132 147 aavaadlvplalgsDTGgSiRqPAsfcgvvGlKPtYGlvSRyGlvayasSldqiGilakkvedialvldvisg 219
                                           aavaa+lvp + g+DTGgSiRqPA++cgv GlKPtYG+vSR+G++a+asSldq G++a++ ed al+l +++g
  NCBI__GCF_900112605.1:WP_093428567.1 157 AAVAAGLVPGTTGTDTGGSIRQPAALCGVTGLKPTYGRVSRWGMIAFASSLDQAGPMARTAEDCALLLGAMAG 229
                                           ************************************************************************* PP

                             TIGR00132 220 kDkkDstslevkveelleelkkdlkglkvgvvkelseesldkevkekfekllekleelgaeivevslpsvkla 292
                                            D++Dstsl+ +v++++e l + l gl++g+ ke+++++ld + ++++e+++e+++ lgaei evslp+ +la
  NCBI__GCF_900112605.1:WP_093428567.1 230 FDERDSTSLQEPVPDYTEGLGRSLEGLRIGLPKEYFGSGLDAGTARALEAAIEEFRGLGAEIREVSLPHAELA 302
                                           ************************************************************************* PP

                             TIGR00132 293 laiYyiispsEassnlarydgiryGkrveelkslkelyaktRsegfgeevkrRimlGayalskeyydkyykkA 365
                                           +++Yy+i+p+E ssnl+r+dg+ryG+r +++++l++ly+++R egfg+evkrR+m+G+yals++y+d+yy+kA
  NCBI__GCF_900112605.1:WP_093428567.1 303 VPTYYVIAPAECSSNLSRFDGVRYGHRCQDPEDLEDLYKRSRGEGFGDEVKRRVMVGTYALSAGYFDAYYLKA 375
                                           ************************************************************************* PP

                             TIGR00132 366 qkvrtliidefeklfeevDvivsptaptlafklgekaedplemylsDvltvpanlaGlpaisvPlgkkekglp 438
                                           q++r+li+++f  +feevD++++ptap  af++gek++ p++mylsD++t+ anlaGlpa+svP+g   +g p
  NCBI__GCF_900112605.1:WP_093428567.1 376 QRMRRLIKNDFMAAFEEVDLLLGPTAPGPAFNIGEKTD-PVSMYLSDIYTIAANLAGLPAMSVPAGLV-DGRP 446
                                           *************************************9.*****************************.7*** PP

                             TIGR00132 439 iGlqiigkafddkkllsvakaleqald 465
                                           +Glq+ig++fd+  ll++a+ ++q +d
  NCBI__GCF_900112605.1:WP_093428567.1 447 VGLQLIGNYFDEPGLLNAAHRYQQVTD 473
                                           **********************99876 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (466 nodes)
Target sequences:                          1  (489 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 18.08
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis