Alignments for a candidate for metX in Thiohalospira halophila DSM 15071 HL 3

GapMind for Amino acid biosynthesis

Alignments for a candidate for metX in Thiohalospira halophila DSM 15071 HL 3

Align Homoserine O-acetyltransferase; HAT; EC 2.3.1.31; Homoserine transacetylase; HTA (uncharacterized)
to candidate WP_093428353.1 BM272_RS08475 homoserine O-acetyltransferase

Query= curated2:Q1QQD3
         (399 letters)



>NCBI__GCF_900112605.1:WP_093428353.1
          Length = 384

 Score =  393 bits (1009), Expect = e-114
 Identities = 188/354 (53%), Positives = 250/354 (70%), Gaps = 2/354 (0%)

Query: 33  DQPLPLDCGIDLAPFQIAYQTYGELNAGKSNAILVCHALTGDQHVANVHPVTG-KPGWWV 91
           DQPL LDCG  L  +++ Y+TYG  NA +SNAILVCHAL+GD H A  + +   KPGWW 
Sbjct: 21  DQPLELDCGRVLPEYELVYETYGTPNADRSNAILVCHALSGDHHAAGYNSMEDRKPGWWE 80

Query: 92  TLVGPGKPLDTDKYFVICSNVIGGCMGSTGPASTNPATGTVWGLDFPVITIPDMVRAQAM 151
             +GPGKPLDT+++F++  N +G C GS+GP + NP TG  WG DFP++T+ D V + A 
Sbjct: 81  ACIGPGKPLDTNRFFIVSVNNLGSCKGSSGPNTINPETGRYWGPDFPIVTVRDWVHSHAR 140

Query: 152 LIDRLGIDTLFSVVGGSMGGMQVLQWCVAYPQRVFSALPIACSTRHSAQNIAFHELGRQA 211
           L D LGI+   +VVGGS+GGMQV+QW + YP R+  A+ IA + R SAQNIAF+E+ RQA
Sbjct: 141 LADELGIEQWAAVVGGSLGGMQVMQWAIDYPDRLRHAVIIAGAPRLSAQNIAFNEVARQA 200

Query: 212 VMADPDWRDGRYVEQGTYPHRGLGVARMAAHITYLSDAALHRKFGRRMQDRDLPTFSFDA 271
           +M+DP++ DG Y+++GT P RGL +ARM  HITYLSD A+  KFGR ++  D   F +D 
Sbjct: 201 IMSDPEFHDGYYLQKGTLPRRGLMLARMLGHITYLSDEAMRAKFGRELR-TDQINFGYDV 259

Query: 272 DFQVESYLRHQGSSFVERFDANSYLYLTRAMDYFDIAADHNGVLAAAFRDTRTRFCVVSF 331
           DFQVESYLR+QG SFV+RFDAN+YL +TRA+DYFD AA+H+  L AA      R  VVSF
Sbjct: 260 DFQVESYLRYQGQSFVDRFDANTYLLMTRALDYFDPAAEHDDDLTAALAPVTARSLVVSF 319

Query: 332 TSDWLFPTSESRATVHALNAGGARVSFAEIETDRGHDAFLLDLPEFFDIAHAFL 385
           TSDW F    SR  V AL  G   V++AE++  +GHDAFL+ + ++  +  A++
Sbjct: 320 TSDWRFSPERSREIVKALLDGNKSVAYAEVQAHQGHDAFLMPIAQYMAVFRAYM 373


Lambda     K      H
   0.323    0.136    0.427 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 463
Number of extensions: 16
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 399
Length of database: 384
Length adjustment: 31
Effective length of query: 368
Effective length of database: 353
Effective search space:   129904
Effective search space used:   129904
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 50 (23.9 bits)

Align candidate WP_093428353.1 BM272_RS08475 (homoserine O-acetyltransferase)
to HMM TIGR01392 (metX: homoserine O-acetyltransferase (EC 2.3.1.31))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01392.hmm
# target sequence database:        /tmp/gapView.3657228.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01392  [M=351]
Accession:   TIGR01392
Description: homoserO_Ac_trn: homoserine O-acetyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
     4e-147  475.8   0.0   4.6e-147  475.6   0.0    1.0  1  NCBI__GCF_900112605.1:WP_093428353.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_900112605.1:WP_093428353.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  475.6   0.0  4.6e-147  4.6e-147       2     350 ..      21     373 ..      20     374 .. 0.97

  Alignments for each domain:
  == domain 1  score: 475.6 bits;  conditional E-value: 4.6e-147
                             TIGR01392   2 eeeltlesGevlsevevayktyGtlnaerdNavlvcHaltgsahvagkadeedk..GWWdellGpgraldtsr 72 
                                           +++l+l++G vl+e+e++y+tyGt na+r+Na+lvcHal+g++h+ag+++ ed+  GWW+  +Gpg++ldt+r
  NCBI__GCF_900112605.1:WP_093428353.1  21 DQPLELDCGRVLPEYELVYETYGTPNADRSNAILVCHALSGDHHAAGYNSMEDRkpGWWEACIGPGKPLDTNR 93 
                                           589********************************************998877778***************** PP

                             TIGR01392  73 yfvvclNvlGsckGstgPlsinpetgkpygaefPlvtirDlvkaqkalldsLgveklaavvGgSlGGmqalew 145
                                           +f+v++N+lGsckGs+gP +inpetg+ +g++fP vt+rD+v+ +++l+d+Lg+e++aavvGgSlGGmq+++w
  NCBI__GCF_900112605.1:WP_093428353.1  94 FFIVSVNNLGSCKGSSGPNTINPETGRYWGPDFPIVTVRDWVHSHARLADELGIEQWAAVVGGSLGGMQVMQW 166
                                           ************************************************************************* PP

                             TIGR01392 146 alsypervkkivvlatsarasaqaiafnevqrqailsDpeyndGeyaeee.qPekGLalARmlalltYrsees 217
                                           a++yp+r++++v +a ++r+saq+iafnev+rqai+sDpe++dG+y +++  P++GL lARml+++tY+s+e+
  NCBI__GCF_900112605.1:WP_093428353.1 167 AIDYPDRLRHAVIIAGAPRLSAQNIAFNEVARQAIMSDPEFHDGYYLQKGtLPRRGLMLARMLGHITYLSDEA 239
                                           ************************************************9999********************* PP

                             TIGR01392 218 leerfgreakseeslassleeefsvesylryqgkkfverFdAnsYllltkaldthdlargrrdslkealkkik 290
                                           ++++fgre +++ ++++ ++ +f+vesylryqg++fv+rFdAn+Yll+t+ald++d a++++d+l++al+ ++
  NCBI__GCF_900112605.1:WP_093428353.1 240 MRAKFGRELRTD-QINFGYDVDFQVESYLRYQGQSFVDRFDANTYLLMTRALDYFDPAAEHDDDLTAALAPVT 311
                                           *******99998.78999******************************************************* PP

                             TIGR01392 291 apvlvvgiesDllftleeqeelakalkaakle..yaeieseeGHDaFllekekveelirefl 350
                                           a++lvv+++sD++f++e+++e++kal + +++  yae++ ++GHDaFl+  ++++++ r+++
  NCBI__GCF_900112605.1:WP_093428353.1 312 ARSLVVSFTSDWRFSPERSREIVKALLDGNKSvaYAEVQAHQGHDAFLMPIAQYMAVFRAYM 373
                                           **************************99888888******************9999888776 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (351 nodes)
Target sequences:                          1  (384 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 16.79
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis