GapMind for Amino acid biosynthesis


Potential Gaps in Amino acid biosynthesis in Thermodesulforhabdus norvegica DSM 9990

Found 2 low-confidence and 7 medium-confidence steps on the best paths for 18 pathways.

Pathway Step Best candidate 2nd candidate
his hisI: phosphoribosyl-ATP pyrophosphatase BM091_RS02930 BM091_RS07235
his hisN: histidinol-phosphate phosphatase BM091_RS12655 BM091_RS00250
met asd-S-perS: putative persulfide forming protein BM091_RS02260
met mesB: Methylcobalamin:homocysteine methyltransferase MesB BM091_RS04305
phe aro-dehydratase: arogenate dehydratase BM091_RS03430 BM091_RS06270
ser serB: phosphoserine phosphatase BM091_RS09805
thr homK: putative homoserine phosphotransferase BM091_RS02580
trp PRAI: phosphoribosylanthranilate isomerase BM091_RS05045 BM091_RS08350
tyr pre-dehydr: prephenate dehydrogenase BM091_RS08660 BM091_RS03430

Confidence: high confidence medium confidence low confidence
? – known gap: despite the lack of a good candidate for this step, this organism (or a related organism) performs the pathway

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.



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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory