Alignments for a candidate for argI in Malonomonas rubra DSM 5091

GapMind for Amino acid biosynthesis

Alignments for a candidate for argI in Malonomonas rubra DSM 5091

Align ornithine carbamoyltransferase (EC 2.1.3.3) (characterized)
to candidate WP_072909608.1 BUB13_RS15125 ornithine carbamoyltransferase

Query= BRENDA::O93656
         (317 letters)



>NCBI__GCF_900142125.1:WP_072909608.1
          Length = 303

 Score =  318 bits (815), Expect = 1e-91
 Identities = 160/303 (52%), Positives = 209/303 (68%), Gaps = 4/303 (1%)

Query: 8   RDLLCLQDYTPEEIWTILETAKMLKIWQKIGKPHRLLEGKTLAMIFQKPSTRTRVSFEVA 67
           +D LCL D++  ++  +   AK LK  QK G+ H+LLEG+TL M+F+K STRTRVSFEV 
Sbjct: 3   KDFLCLSDWSLADLEKMFALAKELKAKQKAGEAHKLLEGQTLGMLFEKSSTRTRVSFEVG 62

Query: 68  MAHLGGHALYLNAQDLQLRRGETIADTARVLSRYVDAIMARVYDHKDVEDLAKYASVPVI 127
           M  LGGHAL+L++ + Q+ RGE I DTARV+ RY D IM R Y    VE+ A+Y   PVI
Sbjct: 63  MFQLGGHALFLSSGNTQMGRGEPIKDTARVMGRYCDGIMIRTYSQAAVEEFAEYCDAPVI 122

Query: 128 NGLSDFSHPCQALADYMTIWEKKGTIKGVKVVYVGDGNNVAHSLMIAGTKLGADVVVATP 187
           NGL+D  HPCQ +AD MT+ E K   + +K  ++GDGNN+A+S + A    G ++ VATP
Sbjct: 123 NGLTDMYHPCQIMADLMTVQEHKEDYRNLKYCWIGDGNNMANSWINAAAIFGFELRVATP 182

Query: 188 EGYEPDEKVIKWAEQNAAESGGSFELLHDPVKAVKDADVIYTDVWASMGQEAEAEERRKI 247
           +GYE D  VI  AE    ++G      +DP +A   ADV+ TDVWASMGQE E  ER K 
Sbjct: 183 KGYEADAAVIARAE----KAGAKLIFTNDPKEAAAGADVLNTDVWASMGQEEEQAEREKA 238

Query: 248 FRPFQVNKDLVKHAKPDYMFMHCLPAHRGEEVTDDVIDSPNSVVWDEAENRLHAQKAVLA 307
           F  FQ+N +LVK A  D + MHCLPAHR EE+TD+VI+ PNS+++DEAENRLH QKA++A
Sbjct: 239 FVGFQINSELVKMAAADCVVMHCLPAHRDEEITDEVIEGPNSIIFDEAENRLHVQKAIMA 298

Query: 308 LLL 310
            L+
Sbjct: 299 SLM 301


Lambda     K      H
   0.319    0.134    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 287
Number of extensions: 10
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 317
Length of database: 303
Length adjustment: 27
Effective length of query: 290
Effective length of database: 276
Effective search space:    80040
Effective search space used:    80040
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 48 (23.1 bits)

Align candidate WP_072909608.1 BUB13_RS15125 (ornithine carbamoyltransferase)
to HMM TIGR00658 (argF: ornithine carbamoyltransferase (EC 2.1.3.3))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00658.hmm
# target sequence database:        /tmp/gapView.1229144.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00658  [M=304]
Accession:   TIGR00658
Description: orni_carb_tr: ornithine carbamoyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   3.1e-129  416.5   0.1   3.5e-129  416.3   0.1    1.0  1  NCBI__GCF_900142125.1:WP_072909608.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_900142125.1:WP_072909608.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  416.3   0.1  3.5e-129  3.5e-129       1     303 [.       3     301 ..       3     302 .. 0.99

  Alignments for each domain:
  == domain 1  score: 416.3 bits;  conditional E-value: 3.5e-129
                             TIGR00658   1 rhllslldlseeelkellelakklkkekkkgkeekklkgktlaliFekrstRtRvsfevaayelGaqvlylnk 73 
                                           +++l+l d+s ++l+++  lak+lk+++k+g+ +k l+g+tl+++Fek+stRtRvsfev++++lG+++l+l++
  NCBI__GCF_900142125.1:WP_072909608.1   3 KDFLCLSDWSLADLEKMFALAKELKAKQKAGEAHKLLEGQTLGMLFEKSSTRTRVSFEVGMFQLGGHALFLSS 75 
                                           79*********************************************************************** PP

                             TIGR00658  74 eelqlgrkesikDtarvlsryvdaivvRvykhedveelakyasvPvingLtdlehPcqilaDlltikeklgkl 146
                                            ++q+gr+e+ikDtarv++ry d+i++R+y++  vee+a+y   PvingLtd+ hPcqi+aDl+t++e++++ 
  NCBI__GCF_900142125.1:WP_072909608.1  76 GNTQMGRGEPIKDTARVMGRYCDGIMIRTYSQAAVEEFAEYCDAPVINGLTDMYHPCQIMADLMTVQEHKEDY 148
                                           ************************************************************************* PP

                             TIGR00658 147 kevklvyvGDannvanslllaaaklGldvvvatPeglepeaeivkkakkiakenggkleltedpkkavkdadv 219
                                            ++k+ ++GD+nn+ans + aaa +G++++vatP+g+e +a+++ +a+k     g+kl +t+dpk+a+++adv
  NCBI__GCF_900142125.1:WP_072909608.1 149 RNLKYCWIGDGNNMANSWINAAAIFGFELRVATPKGYEADAAVIARAEK----AGAKLIFTNDPKEAAAGADV 217
                                           *********************************************9965....9******************* PP

                             TIGR00658 220 iytDvwvsmGeeekkeerlkllkpyqvneellelakpevkflhCLPavrGeevtdevlegeasivfdeaenRl 292
                                           + tDvw+smG+ee+++er k++ ++q+n+el+++a  + +++hCLPa+r ee+tdev+eg++si+fdeaenRl
  NCBI__GCF_900142125.1:WP_072909608.1 218 LNTDVWASMGQEEEQAEREKAFVGFQINSELVKMAAADCVVMHCLPAHRDEEITDEVIEGPNSIIFDEAENRL 290
                                           ************************************************************************* PP

                             TIGR00658 293 haqkavlkall 303
                                           h+qka+++ l+
  NCBI__GCF_900142125.1:WP_072909608.1 291 HVQKAIMASLM 301
                                           *******9887 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (304 nodes)
Target sequences:                          1  (303 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 22.27
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis