Alignments for a candidate for tpiA in Tistlia consotensis USBA 355

GapMind for Amino acid biosynthesis

Alignments for a candidate for tpiA in Tistlia consotensis USBA 355

Align Triosephosphate isomerase; TIM; TPI; Triose-phosphate isomerase; EC 5.3.1.1 (characterized)
to candidate WP_085125508.1 B9O00_RS25305 triose-phosphate isomerase

Query= SwissProt::Q8L1Z5
         (254 letters)



>NCBI__GCF_900177295.1:WP_085125508.1
          Length = 257

 Score =  234 bits (597), Expect = 1e-66
 Identities = 129/253 (50%), Positives = 160/253 (63%), Gaps = 14/253 (5%)

Query: 6   RPFIAGNWKMNGTGESLGELRAIAAGISSDLGRLFEA----------LICVPATLLSRAF 55
           RP IAGNWKMNG    + + R +A  I+  + R  E           L+C P TLL    
Sbjct: 3   RPLIAGNWKMNGR---VADGRLLAEEIAQRMHRARETDEPQLPECDLLVCPPFTLLETVH 59

Query: 56  DILGGENILLGGQNCHFDDYGPYTGDISAFMLKEAGASHVIIGHSERRTVYQESDAIVRA 115
             L G  + LG Q+CH    G +TGDISA ML + G SHVI+GHSERR  + E DA+VRA
Sbjct: 60  RALAGSGVALGAQDCHPAASGAHTGDISAAMLADLGCSHVIVGHSERRHDHGEGDALVRA 119

Query: 116 KVQAAWRAGLVALICVGETLEERKSNKVLDVLTRQLEGSLPDGATAENIIIAYEPVWAVG 175
           K +AA   GLV ++CVGET  ER + + L V+T QL GSLP+    E +++AYEPVWA+G
Sbjct: 120 KAEAALAEGLVPVVCVGETAAERDAGRALAVVTGQLAGSLPESFEPERLVLAYEPVWAIG 179

Query: 176 TGNTATSADVAEVHAFIHHKMHSRFG-DEGAKIRLLYGGSVKPSNAFELLSTAHVNGALI 234
           TG TAT AD AEVH  I   + +RFG    A +R+LYGGSVKP NA ELL    V+GAL+
Sbjct: 180 TGKTATPADAAEVHQAIRGALAARFGAGAAAAVRILYGGSVKPENAAELLRAPEVDGALV 239

Query: 235 GGASLKAIDFLTI 247
           GGASLK+ DF  I
Sbjct: 240 GGASLKSDDFWRI 252


Lambda     K      H
   0.320    0.137    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 242
Number of extensions: 11
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 254
Length of database: 257
Length adjustment: 24
Effective length of query: 230
Effective length of database: 233
Effective search space:    53590
Effective search space used:    53590
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 47 (22.7 bits)

Align candidate WP_085125508.1 B9O00_RS25305 (triose-phosphate isomerase)
to HMM TIGR00419 (tpiA: triose-phosphate isomerase (EC 5.3.1.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00419.hmm
# target sequence database:        /tmp/gapView.1464432.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00419  [M=228]
Accession:   TIGR00419
Description: tim: triose-phosphate isomerase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
    1.7e-61  194.0   0.1      2e-61  193.7   0.1    1.0  1  NCBI__GCF_900177295.1:WP_085125508.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_900177295.1:WP_085125508.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  193.7   0.1     2e-61     2e-61       1     228 []       5     247 ..       5     247 .. 0.91

  Alignments for each domain:
  == domain 1  score: 193.7 bits;  conditional E-value: 2e-61
                             TIGR00419   1 lviinfKlnesvgkvelevaklaeev..asea......gvevavappfvdldvvkdeve.seiqvaAqnvdav 64 
                                           l+ +n+K+n+ v +  l+  ++a+ +  a e+      ++ + v ppf  l++v++++  s + ++Aq+++  
  NCBI__GCF_900177295.1:WP_085125508.1   5 LIAGNWKMNGRVADGRLLAEEIAQRMhrARETdepqlpECDLLVCPPFTLLETVHRALAgSGVALGAQDCHPA 77 
                                           689**********9999999998855224443222233589*****************999************ PP

                             TIGR00419  65 ksGaftGeisAemlkdlGakgvligHsErRsllkeadeliekkvarlkelglksvvCvgetleereaartinn 137
                                            sGa+tG+isA+ml+dlG+ +v++gHsErR  + e d l+ +k   + + gl +vvCvget +er+a+r + +
  NCBI__GCF_900177295.1:WP_085125508.1  78 ASGAHTGDISAAMLADLGCSHVIVGHSERRHDHGEGDALVRAKAEAALAEGLVPVVCVGETAAERDAGRALAV 150
                                           ***********************************************************************99 PP

                             TIGR00419 138 vattaaaaA...lepd..vvAvEPveliGtGkpvskAeaevveksvrdhlkk.vskevaesvrvlyGasvtaa 204
                                           v+ + a      +ep+  v+A+EPv++iGtGk++++A+a +v++ +r  l+    +  a  vr+lyG+sv+ +
  NCBI__GCF_900177295.1:WP_085125508.1 151 VTGQLAGSLpesFEPErlVLAYEPVWAIGTGKTATPADAAEVHQAIRGALAArFGAGAAAAVRILYGGSVKPE 223
                                           98887765567777654499*****************************9987999***************** PP

                             TIGR00419 205 edaelaaqldvdGvLlasavlkae 228
                                           ++ael   ++vdG+L+++a+lk++
  NCBI__GCF_900177295.1:WP_085125508.1 224 NAAELLRAPEVDGALVGGASLKSD 247
                                           **********************86 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (228 nodes)
Target sequences:                          1  (257 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 21.52
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis