Protein 353923 in Bacteroides thetaiotaomicron VPI-5482
Annotation: FitnessBrowser__Btheta:353923
Length: 460 amino acids
Source: Btheta in FitnessBrowser
Candidate for 22 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| D-xylose catabolism | xylT | med | D-xylose-proton symporter (characterized) | 39% | 96% | 332.4 | D-fructose transporter, sugar porter family | 36% | 288.5 |
| L-arabinose catabolism | araE | lo | Arabinose-proton symporter; Arabinose transporter (characterized) | 37% | 97% | 327.4 | D-xylose-proton symporter | 39% | 332.4 |
| D-galactose catabolism | galP | lo | Arabinose-proton symporter; Arabinose transporter (characterized) | 37% | 97% | 327.4 | D-xylose-proton symporter | 39% | 332.4 |
| D-fructose catabolism | glcP | lo | D-fructose transporter, sugar porter family (characterized) | 36% | 93% | 288.5 | D-xylose-proton symporter | 39% | 332.4 |
| sucrose catabolism | glcP | lo | D-fructose transporter, sugar porter family (characterized) | 36% | 93% | 288.5 | D-xylose-proton symporter | 39% | 332.4 |
| D-cellobiose catabolism | MFS-glucose | lo | The glucose uptake porter, GluP (characterized) | 37% | 95% | 285.8 | D-xylose-proton symporter | 39% | 332.4 |
| D-glucose catabolism | MFS-glucose | lo | The glucose uptake porter, GluP (characterized) | 37% | 95% | 285.8 | D-xylose-proton symporter | 39% | 332.4 |
| lactose catabolism | MFS-glucose | lo | The glucose uptake porter, GluP (characterized) | 37% | 95% | 285.8 | D-xylose-proton symporter | 39% | 332.4 |
| D-maltose catabolism | MFS-glucose | lo | The glucose uptake porter, GluP (characterized) | 37% | 95% | 285.8 | D-xylose-proton symporter | 39% | 332.4 |
| sucrose catabolism | MFS-glucose | lo | The glucose uptake porter, GluP (characterized) | 37% | 95% | 285.8 | D-xylose-proton symporter | 39% | 332.4 |
| trehalose catabolism | MFS-glucose | lo | The glucose uptake porter, GluP (characterized) | 37% | 95% | 285.8 | D-xylose-proton symporter | 39% | 332.4 |
| myo-inositol catabolism | iolT | lo | Major myo-inositol transporter IolT (characterized) | 37% | 95% | 258.8 | D-xylose-proton symporter | 39% | 332.4 |
| D-sorbitol (glucitol) catabolism | SOT | lo | Sorbitol (D-Glucitol):H+ co-transporter, SOT1 (Km for sorbitol of 0.64 mM) of 509 aas and 12 TMSs (Gao et al. 2003). SOT1 of P. cerasus is expressed throughout fruit development, but especially when growth and sorbitol accumulation rates are highest. In leaves, PcSOT1 expression is highest in young, expanding tissues, but substantially less in mature leaves (characterized) | 30% | 92% | 211.8 | D-xylose-proton symporter | 39% | 332.4 |
| xylitol catabolism | PLT5 | lo | Polyol (xylitol):H+ symporter, PLT4 (characterized) | 31% | 90% | 209.1 | D-xylose-proton symporter | 39% | 332.4 |
| D-galactose catabolism | gal2 | lo | galactose transporter (characterized) | 30% | 80% | 199.1 | D-xylose-proton symporter | 39% | 332.4 |
| D-xylose catabolism | gal2 | lo | galactose transporter (characterized) | 30% | 80% | 199.1 | D-xylose-proton symporter | 39% | 332.4 |
| trehalose catabolism | TRET1 | lo | Facilitated trehalose transporter Tret1; BmTRET1 (characterized) | 31% | 77% | 198.4 | D-xylose-proton symporter | 39% | 332.4 |
| D-fructose catabolism | Slc2a5 | lo | sugar transport protein 13 (characterized) | 30% | 88% | 189.9 | D-xylose-proton symporter | 39% | 332.4 |
| sucrose catabolism | Slc2a5 | lo | sugar transport protein 13 (characterized) | 30% | 88% | 189.9 | D-xylose-proton symporter | 39% | 332.4 |
| D-galacturonate catabolism | gatA | lo | The galacturonic acid (galacturonate) uptake porter, GatA, of 518 aas and 12 TMSs (characterized) | 30% | 91% | 180.6 | D-xylose-proton symporter | 39% | 332.4 |
| D-gluconate catabolism | ght3 | lo | high-affinity gluconate transporter ght3 (characterized) | 31% | 84% | 179.5 | D-xylose-proton symporter | 39% | 332.4 |
| myo-inositol catabolism | HMIT | lo | Proton myo-inositol cotransporter; H(+)-myo-inositol cotransporter; Hmit; H(+)-myo-inositol symporter; Solute carrier family 2 member 13 (characterized) | 34% | 52% | 176.4 | D-xylose-proton symporter | 39% | 332.4 |
Sequence Analysis Tools
View 353923 at FitnessBrowser
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
Sequence
MKSYNKKFVYSICLVSAMGGLLFGYDWVVIGGAKPFYELYFGIADSPTMQGLAMSVALLG
CLIGAMVAGMMADRYGRKPLLLISAFIFLSSAYATGAFSVFSWFLAARFLGGIGIGIASG
LSPMYIAEVAPTSIRGKLVSLNQLTIVLGILGAQIANWLIAEPIPADFTPADICASWNGQ
MGWRWMFWGAAFPAAVFLLLACFIPESPRWLAMKGKREKAWSVLSRIGGNRYAEQELQMV
EQTSASKSEGGLKLLFSRPFRKVLVLGVIVAVFQQWCGTNVIFNYAQEIFQSAGYSLGDV
LFNIVVTGVANVIFTFVAIYTVERLGRRALMLLGAGGLAGIYLVLGTCYFFQVSGFFMVV
LVVLAIACYAMSLGPITWVLLAEIFPNRVRGVAMATCTFALWVGSFTLTYTFPLLNTALG
SYGTFWIYSAICVFGFLFFLRALPETKGKSLETLEKDLIK
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory