Annotation: FitnessBrowser__Burk376:H281DRAFT_03586
Length: 343 amino acids
Source: Burk376 in FitnessBrowser
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|---|---|---|---|---|---|---|---|---|
| L-threonine catabolism | tdh | hi | L-threonine 3-dehydrogenase (EC 1.1.1.103) (characterized) | 76% | 100% | 552.7 | L-iditol 2-dehydrogenase; EC 1.1.1.14 | 33% | 188.7 |
| L-threonine catabolism | tdh | hi | tdh: L-threonine 3-dehydrogenase (EC 1.1.1.103) (TIGR00692) | 100% | 538.2 | L-iditol 2-dehydrogenase; EC 1.1.1.14 | 33% | 188.7 | |
| xylitol catabolism | xdhA | lo | L-iditol 2-dehydrogenase; EC 1.1.1.14 (characterized) | 33% | 97% | 188.7 | L-threonine 3-dehydrogenase (EC 1.1.1.103) | 76% | 552.7 |
| D-xylose catabolism | xdhA | lo | L-iditol 2-dehydrogenase; EC 1.1.1.14 (characterized) | 33% | 97% | 188.7 | L-threonine 3-dehydrogenase (EC 1.1.1.103) | 76% | 552.7 |
| xylitol catabolism | x5p-reductase | lo | Lmo2663 protein (characterized, see rationale) | 32% | 99% | 158.7 | L-threonine 3-dehydrogenase (EC 1.1.1.103) | 76% | 552.7 |
View H281DRAFT_03586 at FitnessBrowser
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Find functional residues: SitesBLAST
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Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
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MKALAKLERAPGLTLTDVKVPEVGHNDVMIRIKRTAICGTDIHIWKWDDWAQKTIPVPMH VGHEYVGEIVEMGAEVRGFSIGDRVSGEGHITCGFCRNCRAGRRHLCRNTVGVGVNREGA FAEYLVIPAFNAFKIPPEISDDLAAIFDPFGNATHTALSFNLVGEDVLITGAGPIGIMAV AIAKHVGARNVVITDINDYRLGLARKMGATRAVNVSRESLRDVMADLHMAEGFDVGLEMS GVPGAFTGMLEAMNHGGKIALLGIPPAQTAIDWNQVIFKGLEIKGIYGREMFETWYKMVA MLQSGLDLSPILTHHFKVDDYQQAFATMLSGESGKVILDWTSA
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory