Alignments for a candidate for aacS in Paraburkholderia bryophila 376MFSha3.1

GapMind for catabolism of small carbon sources

Alignments for a candidate for aacS in Paraburkholderia bryophila 376MFSha3.1

Align Acetoacetate--CoA ligase (EC 6.2.1.16) (characterized)
to candidate H281DRAFT_04125 H281DRAFT_04125 Acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II

Query= reanno::acidovorax_3H11:Ac3H11_3009
         (578 letters)



>FitnessBrowser__Burk376:H281DRAFT_04125
          Length = 520

 Score =  217 bits (552), Expect = 1e-60
 Identities = 178/563 (31%), Positives = 267/563 (47%), Gaps = 63/563 (11%)

Query: 16  SHARGATDVPLIEQTIGAFFADMVA----RQPEREALVSVHQGRRYTYAQLQTEAHRLAS 71
           S  R   DVP +   + A  +D+ A    R P+  AL+    GRR T AQL       A+
Sbjct: 5   SPLRSRIDVPALLAALPARISDIPALAAGRDPQHVALIE--DGRRLTNAQLVQAVEATAA 62

Query: 72  ALLGMGLTPGDRVGIWSHNNAEWVLMQLATAQVGLVLVNINPAYRTAEVEYALNKVGCKL 131
            L   G+  GDRV I + N+   +++  ATA                       ++    
Sbjct: 63  LLRDWGVRGGDRVMIVAENSIAQIVLLFATA-----------------------RLDAWA 99

Query: 132 LVSMARFKTSDYLGMLRELAPEWQGQQPGHLQAAKLPQLKT-VVWIDDEAGQGADEPGLL 190
           LVS AR   S+ L  +R            H Q    P++   VV    +AGQ A   G  
Sbjct: 100 LVSNARLSASE-LDAIR-----------AHAQ----PRVTAYVVESSADAGQHAQRHGAS 143

Query: 191 RFTELIARGNAADPRLAQVAAGLQATDPIN------IQFTSGTTGFPKGATLTHRNILNN 244
              + +A G  A        A  +  +  N      + +T+GTTG PKG  L+HRN+L  
Sbjct: 144 AAPD-VAPGIGAWSFTVDTFAQAEPVEAANDRQCAALIYTTGTTGAPKGVMLSHRNLLFI 202

Query: 245 GFFIGECMKLTPADRLCIPVPLYHCFGMVLGNLACFTHGATI-VYPNDGFDPLTVLQTVQ 303
                   ++ P D +   +P+ H +G     L     GAT+ + P   F P  V + + 
Sbjct: 203 AAVSSRLRQVRPDDVVYAVLPISHVYGFASVCLGSLYAGATLRLAPR--FAPGAVRRALA 260

Query: 304 DERCTGLHGVPTMFIAELDHPRFA--EFNLSTLRTGIMAGSPCPTEVMKRVVEQMNLREI 361
           DER +   GVP M    ++H +     ++   LR     GSP     +K  VE +    +
Sbjct: 261 DERVSIFQGVPAMHAKLIEHLQTHGHAWHAPHLRFIYSGGSPLDA-ALKAHVESVYGLPL 319

Query: 362 TIAYGMTETSPVSCQSSTDTPLSKRVSTVGQVQPHLEVKIVDPDTGAVVPIGQRGEFCTK 421
              YGMTE+SP   Q+  D P  +R  +VG+V P +EV+ V  D GA V +G+ GE   +
Sbjct: 320 HNGYGMTESSPTVSQTMLDAP--RRDCSVGEVIPGVEVRFVGLD-GADVAVGETGELWVR 376

Query: 422 GYSVMHGYWGDEAKTREAIDEGGWMHTGDLATMDAEGYVNIVGRIKDMVIRGGENIYPRE 481
           G +VM GY+ +  +TR A+ + GW+ TGDLA  DA+G ++IVGR KD++IR G N+YP E
Sbjct: 377 GPNVMLGYYRNPQQTRAAVTQDGWLKTGDLARQDADGALHIVGRSKDLIIRSGFNVYPAE 436

Query: 482 IEEFLYRHPQVQDVQVVGVPDQKYGEELCAWIIAKPGTQPTEDDIRAFCKGQIAHYKVPR 541
           +E  L  HPQV    V+G P +   EE+ A++        T  ++ A+C  ++A YK P 
Sbjct: 437 VEHVLNAHPQVVQSAVIGRPVEG-NEEVIAFVELAANASVTPAELTAWCVERLAPYKRPA 495

Query: 542 YIRFVTSFPMTVTGKIQKFKIRD 564
            ++ + + P   TGKI K ++R+
Sbjct: 496 EVKVLAALPAASTGKILKHRLRE 518


Lambda     K      H
   0.320    0.136    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 652
Number of extensions: 33
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 578
Length of database: 520
Length adjustment: 36
Effective length of query: 542
Effective length of database: 484
Effective search space:   262328
Effective search space used:   262328
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources